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Fast determination of ethylene glycol, 1,2-propylene glycol and glycolic acid in blood serum and urine for emergency and clinical toxicology by GC-FID.
Talanta. 2014 Dec; 130:470-4.T

Abstract

A simple, cost effective, and fast gas chromatography method with flame ionization detection (GC-FID) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes. This new method employs a relatively less used class of derivatization agents - alkyl chloroformates, allowing the efficient and rapid derivatization of carboxylic acids within seconds while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. To avoid possible interference from naturally occurring endogenous acids and quantitation errors 3-(4-chlorophenyl) propionic acid was chosen as an internal standard. The significant parameters of the derivatization have been found using chemometric procedures and these parameters were optimized using the face-centered central composite design. The calibration dependence of the method was proved to be quadratic in the range of 50-5000 mg mL(-1), with adequate accuracy (92.4-108.7%) and precision (9.4%). The method was successfully applied to quantify the selected compounds in serum of patients from emergency units.

Authors+Show Affiliations

Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic. Electronic address: tomas.hlozek@vfn.cz.Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University in Prague and General University Hospital, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25159437

Citation

Hložek, Tomáš, et al. "Fast Determination of Ethylene Glycol, 1,2-propylene Glycol and Glycolic Acid in Blood Serum and Urine for Emergency and Clinical Toxicology By GC-FID." Talanta, vol. 130, 2014, pp. 470-4.
Hložek T, Bursová M, Čabalaa R. Fast determination of ethylene glycol, 1,2-propylene glycol and glycolic acid in blood serum and urine for emergency and clinical toxicology by GC-FID. Talanta. 2014;130:470-4.
Hložek, T., Bursová, M., & Čabalaa, R. (2014). Fast determination of ethylene glycol, 1,2-propylene glycol and glycolic acid in blood serum and urine for emergency and clinical toxicology by GC-FID. Talanta, 130, 470-4. https://doi.org/10.1016/j.talanta.2014.07.020
Hložek T, Bursová M, Čabalaa R. Fast Determination of Ethylene Glycol, 1,2-propylene Glycol and Glycolic Acid in Blood Serum and Urine for Emergency and Clinical Toxicology By GC-FID. Talanta. 2014;130:470-4. PubMed PMID: 25159437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast determination of ethylene glycol, 1,2-propylene glycol and glycolic acid in blood serum and urine for emergency and clinical toxicology by GC-FID. AU - Hložek,Tomáš, AU - Bursová,Miroslava, AU - Čabalaa,Radomír, Y1 - 2014/07/15/ PY - 2014/05/12/received PY - 2014/07/02/revised PY - 2014/07/07/accepted PY - 2014/8/28/entrez PY - 2014/8/28/pubmed PY - 2015/5/13/medline KW - Chloroformate KW - Experimental design KW - GC KW - Glycolic acid KW - Glycols SP - 470 EP - 4 JF - Talanta JO - Talanta VL - 130 N2 - A simple, cost effective, and fast gas chromatography method with flame ionization detection (GC-FID) for simultaneous measurement of ethylene glycol, 1,2-propylene glycol and glycolic acid was developed and validated for clinical toxicology purposes. This new method employs a relatively less used class of derivatization agents - alkyl chloroformates, allowing the efficient and rapid derivatization of carboxylic acids within seconds while glycols are simultaneously derivatized by phenylboronic acid. The entire sample preparation procedure is completed within 10 min. To avoid possible interference from naturally occurring endogenous acids and quantitation errors 3-(4-chlorophenyl) propionic acid was chosen as an internal standard. The significant parameters of the derivatization have been found using chemometric procedures and these parameters were optimized using the face-centered central composite design. The calibration dependence of the method was proved to be quadratic in the range of 50-5000 mg mL(-1), with adequate accuracy (92.4-108.7%) and precision (9.4%). The method was successfully applied to quantify the selected compounds in serum of patients from emergency units. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/25159437/Fast_determination_of_ethylene_glycol_12_propylene_glycol_and_glycolic_acid_in_blood_serum_and_urine_for_emergency_and_clinical_toxicology_by_GC_FID_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(14)00575-X DB - PRIME DP - Unbound Medicine ER -