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Dietary supplementation of probiotics affects growth, immune response and disease resistance of Cyprinus carpio fry.
Fish Shellfish Immunol. 2014 Dec; 41(2):113-9.FS

Abstract

The effects of dietary Bacillus coagulans (MTCC 9872), Bacillus licheniformis (MTCC 6824) and Paenibacillus polymyxa (MTCC 122) supplementation on growth performance, non-specific immunity and protection against Aeromonas hydrophila infection were evaluated in common carp, Cyprinus carpio fry. Laboratory maintained B. coagulans, B. licheniformis and P. polymyxa were used to study antagonistic activity against fish pathogenic bacteria by agar well diffusion assay. Healthy fish fry were challenged by this bacterium for determination of its safety. Fish were fed for 80 days with control basal diet (B0) and experimental diets containing B. coagulans (B1), B. licheniformis (B2) and P. polymyxa (B3) at 10(9) CFU/g diet. Fish fry (mean weight 0.329 ± 0.01 g) were fed these diets and growth performance, various non-specific immune parameters and disease resistance study were conducted at 80 days post-feeding. The antagonism study showed inhibition zone against A. hydrophila and Vibrio harveyi. All the probiotic bacterial strains were harmless to fish fry as neither mortality nor morbidities were observed of the challenge. The growth-promoting influences of probiotic supplemented dietary treatments were observed with fish fry and the optimum survival, growth and feed utilization were obtained with P. polymyxa (B3) supplemented diet. Study of different non-specific innate immunological parameters viz. lysozyme activity, respiratory burst assay and myeloperoxidase content showed significant (p < 0.05) higher values in fish fry fed B3 diet at 10(9) CFU/g. The challenge test showed dietary supplementation of B. coagulans, B. licheniformis and P. polymyxa significantly (p < 0.05) enhanced the resistance of fish fry against bacterial challenge. These results collectively suggests that P. polymyxa is a potential probiotic species and can be used in aquaculture to improve growth, feed utilization, non-specific immune responses and disease resistance of fry common carp, C. carpio.

Authors+Show Affiliations

Department of Aquatic Environment, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141 004, Punjab, India. Electronic address: drakhilgupta@yahoo.com.Department of Aquaculture, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141 004, Punjab, India. Electronic address: drparomitagupta@yahoo.com.Department of Aquaculture, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141 004, Punjab, India. Electronic address: dhawanasha@gmail.com.

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25160796

Citation

Gupta, Akhil, et al. "Dietary Supplementation of Probiotics Affects Growth, Immune Response and Disease Resistance of Cyprinus Carpio Fry." Fish & Shellfish Immunology, vol. 41, no. 2, 2014, pp. 113-9.
Gupta A, Gupta P, Dhawan A. Dietary supplementation of probiotics affects growth, immune response and disease resistance of Cyprinus carpio fry. Fish Shellfish Immunol. 2014;41(2):113-9.
Gupta, A., Gupta, P., & Dhawan, A. (2014). Dietary supplementation of probiotics affects growth, immune response and disease resistance of Cyprinus carpio fry. Fish & Shellfish Immunology, 41(2), 113-9. https://doi.org/10.1016/j.fsi.2014.08.023
Gupta A, Gupta P, Dhawan A. Dietary Supplementation of Probiotics Affects Growth, Immune Response and Disease Resistance of Cyprinus Carpio Fry. Fish Shellfish Immunol. 2014;41(2):113-9. PubMed PMID: 25160796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary supplementation of probiotics affects growth, immune response and disease resistance of Cyprinus carpio fry. AU - Gupta,Akhil, AU - Gupta,Paromita, AU - Dhawan,Asha, Y1 - 2014/08/23/ PY - 2014/04/30/received PY - 2014/07/30/revised PY - 2014/08/07/accepted PY - 2014/8/28/entrez PY - 2014/8/28/pubmed PY - 2015/7/28/medline KW - Common carp (Cyprinus carpio) KW - Disease resistance KW - Growth performance KW - Immune response KW - Probiotics SP - 113 EP - 9 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 41 IS - 2 N2 - The effects of dietary Bacillus coagulans (MTCC 9872), Bacillus licheniformis (MTCC 6824) and Paenibacillus polymyxa (MTCC 122) supplementation on growth performance, non-specific immunity and protection against Aeromonas hydrophila infection were evaluated in common carp, Cyprinus carpio fry. Laboratory maintained B. coagulans, B. licheniformis and P. polymyxa were used to study antagonistic activity against fish pathogenic bacteria by agar well diffusion assay. Healthy fish fry were challenged by this bacterium for determination of its safety. Fish were fed for 80 days with control basal diet (B0) and experimental diets containing B. coagulans (B1), B. licheniformis (B2) and P. polymyxa (B3) at 10(9) CFU/g diet. Fish fry (mean weight 0.329 ± 0.01 g) were fed these diets and growth performance, various non-specific immune parameters and disease resistance study were conducted at 80 days post-feeding. The antagonism study showed inhibition zone against A. hydrophila and Vibrio harveyi. All the probiotic bacterial strains were harmless to fish fry as neither mortality nor morbidities were observed of the challenge. The growth-promoting influences of probiotic supplemented dietary treatments were observed with fish fry and the optimum survival, growth and feed utilization were obtained with P. polymyxa (B3) supplemented diet. Study of different non-specific innate immunological parameters viz. lysozyme activity, respiratory burst assay and myeloperoxidase content showed significant (p < 0.05) higher values in fish fry fed B3 diet at 10(9) CFU/g. The challenge test showed dietary supplementation of B. coagulans, B. licheniformis and P. polymyxa significantly (p < 0.05) enhanced the resistance of fish fry against bacterial challenge. These results collectively suggests that P. polymyxa is a potential probiotic species and can be used in aquaculture to improve growth, feed utilization, non-specific immune responses and disease resistance of fry common carp, C. carpio. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/25160796/Dietary_supplementation_of_probiotics_affects_growth_immune_response_and_disease_resistance_of_Cyprinus_carpio_fry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(14)00308-8 DB - PRIME DP - Unbound Medicine ER -