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Development of T. aestivum L.-H. californicum alien chromosome lines and assignment of homoeologous groups of Hordeum californicum chromosomes.
J Genet Genomics. 2014 Aug 20; 41(8):439-47.JG

Abstract

Hordeum californicum (2n = 2x = 14, HH) is resistant to several wheat diseases and tolerant to lower nitrogen. In this study, a molecular karyotype of H. californicum chromosomes in the Triticum aestivum L. cv. Chinese Spring (CS)-H. californicum amphidiploid (2n = 6x = 56, AABBDDHH) was established. By genomic in situ hybridization (GISH) and multicolor fluorescent in situ hybridization (FISH) using repetitive DNA clones (pTa71, pTa794 and pSc119.2) as probes, the H. californicum chromosomes could be differentiated from each other and from the wheat chromosomes unequivocally. Based on molecular karyotype and marker analyses, 12 wheat-alien chromosome lines, including four disomic addition lines (DAH1, DAH3, DAH5 and DAH6), five telosomic addition lines (MtH7L, MtH1S, MtH1L, DtH6S and DtH6L), one multiple addition line involving H. californicum chromosome H2, one disomic substitution line (DSH4) and one translocation line (TH7S/1BL), were identified from the progenies derived from the crosses of CS-H. californicum amphidiploid with common wheat varieties. A total of 482 EST (expressed sequence tag) or SSR (simple sequence repeat) markers specific for individual H. californicum chromosomes were identified, and 47, 50, 45, 49, 21, 51 and 40 markers were assigned to chromosomes H1, H2, H3, H4, H5, H6 and H7, respectively. According to the chromosome allocation of these markers, chromosomes H2, H3, H4, H5, and H7 of H. californicum have relationship with wheat homoeologous groups 5, 2, 6, 3, and 1, and hence could be designated as 5H(c), 2H(c), 6H(c), 3H(c) and 1H(c), respectively. The chromosomes H1 and H6 were designated as 7H(c) and 4H(c), respectively, by referring to SSR markers located on rye chromosomes.

Authors+Show Affiliations

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China; Wheat Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China.Wheat Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.Wheat Research Center, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: xiuew@njau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25160976

Citation

Fang, Yuhui, et al. "Development of T. Aestivum L.-H. Californicum Alien Chromosome Lines and Assignment of Homoeologous Groups of Hordeum Californicum Chromosomes." Journal of Genetics and Genomics = Yi Chuan Xue Bao, vol. 41, no. 8, 2014, pp. 439-47.
Fang Y, Yuan J, Wang Z, et al. Development of T. aestivum L.-H. californicum alien chromosome lines and assignment of homoeologous groups of Hordeum californicum chromosomes. J Genet Genomics. 2014;41(8):439-47.
Fang, Y., Yuan, J., Wang, Z., Wang, H., Xiao, J., Yang, Z., Zhang, R., Qi, Z., Xu, W., Hu, L., & Wang, X. E. (2014). Development of T. aestivum L.-H. californicum alien chromosome lines and assignment of homoeologous groups of Hordeum californicum chromosomes. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 41(8), 439-47. https://doi.org/10.1016/j.jgg.2014.06.004
Fang Y, et al. Development of T. Aestivum L.-H. Californicum Alien Chromosome Lines and Assignment of Homoeologous Groups of Hordeum Californicum Chromosomes. J Genet Genomics. 2014 Aug 20;41(8):439-47. PubMed PMID: 25160976.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of T. aestivum L.-H. californicum alien chromosome lines and assignment of homoeologous groups of Hordeum californicum chromosomes. AU - Fang,Yuhui, AU - Yuan,Jingya, AU - Wang,Zhangjun, AU - Wang,Haiyan, AU - Xiao,Jin, AU - Yang,Zhixi, AU - Zhang,Ruiqi, AU - Qi,Zengjun, AU - Xu,Weigang, AU - Hu,Lin, AU - Wang,Xiu-E, Y1 - 2014/06/23/ PY - 2014/01/20/received PY - 2014/06/04/revised PY - 2014/06/16/accepted PY - 2014/8/28/entrez PY - 2014/8/28/pubmed PY - 2015/4/7/medline KW - Alien chromosome lines KW - Chinese Spring–H. californicum amphiploid KW - GISH/FISH KW - Homoeologous group KW - Hordeum californicum SP - 439 EP - 47 JF - Journal of genetics and genomics = Yi chuan xue bao JO - J Genet Genomics VL - 41 IS - 8 N2 - Hordeum californicum (2n = 2x = 14, HH) is resistant to several wheat diseases and tolerant to lower nitrogen. In this study, a molecular karyotype of H. californicum chromosomes in the Triticum aestivum L. cv. Chinese Spring (CS)-H. californicum amphidiploid (2n = 6x = 56, AABBDDHH) was established. By genomic in situ hybridization (GISH) and multicolor fluorescent in situ hybridization (FISH) using repetitive DNA clones (pTa71, pTa794 and pSc119.2) as probes, the H. californicum chromosomes could be differentiated from each other and from the wheat chromosomes unequivocally. Based on molecular karyotype and marker analyses, 12 wheat-alien chromosome lines, including four disomic addition lines (DAH1, DAH3, DAH5 and DAH6), five telosomic addition lines (MtH7L, MtH1S, MtH1L, DtH6S and DtH6L), one multiple addition line involving H. californicum chromosome H2, one disomic substitution line (DSH4) and one translocation line (TH7S/1BL), were identified from the progenies derived from the crosses of CS-H. californicum amphidiploid with common wheat varieties. A total of 482 EST (expressed sequence tag) or SSR (simple sequence repeat) markers specific for individual H. californicum chromosomes were identified, and 47, 50, 45, 49, 21, 51 and 40 markers were assigned to chromosomes H1, H2, H3, H4, H5, H6 and H7, respectively. According to the chromosome allocation of these markers, chromosomes H2, H3, H4, H5, and H7 of H. californicum have relationship with wheat homoeologous groups 5, 2, 6, 3, and 1, and hence could be designated as 5H(c), 2H(c), 6H(c), 3H(c) and 1H(c), respectively. The chromosomes H1 and H6 were designated as 7H(c) and 4H(c), respectively, by referring to SSR markers located on rye chromosomes. SN - 1673-8527 UR - https://www.unboundmedicine.com/medline/citation/25160976/Development_of_T__aestivum_L__H__californicum_alien_chromosome_lines_and_assignment_of_homoeologous_groups_of_Hordeum_californicum_chromosomes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1673-8527(14)00117-9 DB - PRIME DP - Unbound Medicine ER -