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[Flow cytometric analysis of surface phenotypes in B-cell non-Hodgkin's lymphoma].
Rinsho Ketsueki. 1989 Dec; 30(12):2134-40.RK

Abstract

The objective of this study is to demonstrate the diagnostic usefulness of flow cytometric analysis of surface immunoglobulin (S-Ig) light chains and monoclonal antibodies (MoAbs) in B-cell non-Hodgkin's lymphoma. For this purpose, the biopsied specimen (lymph nodes, tonsils, and spleens etc) cell suspensions from 44 patients were studied to detect the expression of S-Ig and several antigens recognized by MoAbs. A tumor was considered B-lineage if expression of pan B antigens and/or monoclonal light chains (S-Ig and/or cytoplasmic Ig) was detected in the absence of pan T antigens. Monoclonal expression of S-Ig light chain was detectable in 37 of 44 (84%) patients with B-cell lymphoma. In two of three cases having polyclonal S-Ig light chain, monoclonal C-Ig light chain was detected in large cells on paraffin-embedded tissues with immunohistochemical technique. Pan B antigens were strongly positive in four cases with negative S-Ig light chain. In diffuse large cell lymphoma, expression of CD11b was restricted to non-cleaved cell type (DLN). CD10 was favorably expressed by non-cleaved cell type and to a lesser extent, by immunoblastic histology (LI), but was not detected on cases with cleaved cell type (DLC). Furthermore, relatively uniform expression of S-IgM was seen in DLC subgroup, and on the contrary, heterogenous S-Ig was shown in other histology groups (DLN, LI). Flow cytometry provides a rapid, objective technology to confirm the immunological diagnosis of B-cell non-Hodgkin's lymphoma.

Authors

No affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

jpn

PubMed ID

2516139

Citation

Chubachi, A. "[Flow Cytometric Analysis of Surface Phenotypes in B-cell non-Hodgkin's Lymphoma]." [Rinsho Ketsueki] the Japanese Journal of Clinical Hematology, vol. 30, no. 12, 1989, pp. 2134-40.
Chubachi A. [Flow cytometric analysis of surface phenotypes in B-cell non-Hodgkin's lymphoma]. Rinsho Ketsueki. 1989;30(12):2134-40.
Chubachi, A. (1989). [Flow cytometric analysis of surface phenotypes in B-cell non-Hodgkin's lymphoma]. [Rinsho Ketsueki] the Japanese Journal of Clinical Hematology, 30(12), 2134-40.
Chubachi A. [Flow Cytometric Analysis of Surface Phenotypes in B-cell non-Hodgkin's Lymphoma]. Rinsho Ketsueki. 1989;30(12):2134-40. PubMed PMID: 2516139.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Flow cytometric analysis of surface phenotypes in B-cell non-Hodgkin's lymphoma]. A1 - Chubachi,A, PY - 1989/12/1/pubmed PY - 1989/12/1/medline PY - 1989/12/1/entrez SP - 2134 EP - 40 JF - [Rinsho ketsueki] The Japanese journal of clinical hematology JO - Rinsho Ketsueki VL - 30 IS - 12 N2 - The objective of this study is to demonstrate the diagnostic usefulness of flow cytometric analysis of surface immunoglobulin (S-Ig) light chains and monoclonal antibodies (MoAbs) in B-cell non-Hodgkin's lymphoma. For this purpose, the biopsied specimen (lymph nodes, tonsils, and spleens etc) cell suspensions from 44 patients were studied to detect the expression of S-Ig and several antigens recognized by MoAbs. A tumor was considered B-lineage if expression of pan B antigens and/or monoclonal light chains (S-Ig and/or cytoplasmic Ig) was detected in the absence of pan T antigens. Monoclonal expression of S-Ig light chain was detectable in 37 of 44 (84%) patients with B-cell lymphoma. In two of three cases having polyclonal S-Ig light chain, monoclonal C-Ig light chain was detected in large cells on paraffin-embedded tissues with immunohistochemical technique. Pan B antigens were strongly positive in four cases with negative S-Ig light chain. In diffuse large cell lymphoma, expression of CD11b was restricted to non-cleaved cell type (DLN). CD10 was favorably expressed by non-cleaved cell type and to a lesser extent, by immunoblastic histology (LI), but was not detected on cases with cleaved cell type (DLC). Furthermore, relatively uniform expression of S-IgM was seen in DLC subgroup, and on the contrary, heterogenous S-Ig was shown in other histology groups (DLN, LI). Flow cytometry provides a rapid, objective technology to confirm the immunological diagnosis of B-cell non-Hodgkin's lymphoma. SN - 0485-1439 UR - https://www.unboundmedicine.com/medline/citation/2516139/[Flow_cytometric_analysis_of_surface_phenotypes_in_B_cell_non_Hodgkin's_lymphoma]_ DB - PRIME DP - Unbound Medicine ER -