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Modulatory role of kolaviron in phenytoin-induced hepatic and testicular dysfunctions in Wistar rats.
J Diet Suppl. 2015 Mar; 12(1):105-17.JD

Abstract

Phenytoin, an anticonvulsant agent used for the treatment of epilepsy has been reported to exhibit toxic side effects on the liver and testes. The present study investigated the protective effects of kolaviron (KV, a bioflavonoid from Garcinia kola seeds) against hepatic and testicular damage in rats exposed to phenytoin. The study consisted of four groups of six rats per group. Group I rats received 2 mL/kg of corn alone while group II received 75 mg/kg of phenytoin (PHT) alone. Groups III and IV were co-treated with kolaviron (200 mg/kg KV) and vitamin E (500 mg/kg VTE), respectively, for 14 days. The antioxidant status, hepatic and reproductive functional parameters were subsequently determined. PHT treatment significantly (p < 0.05) increased superoxide dismutase (SOD) and catalase (CAT) activities, elevated lipid peroxidation (LPO) and hydrogen peroxide (H2O2) levels along with significant reduction in the hepatic and testicular levels of glutathione (GSH). Moreover, PHT exposure elicited significant increases in alkaline phosphatase (ALP) and aspartate aminotransferase (AST) levels. The significant reduction in seminal epithelium thickness and the diameter of seminiferous tubules was accompanied with marked decrease in sperm motility, sperm count, and viability in PHT-treated rats. However, antioxidant status and the functional indices of liver and testes were restored to near control levels in rats co-treated with KV and VTE. In conclusion, KV and VTE protect the liver and testes against functional impairment due to PHT treatment.

Authors+Show Affiliations

1Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan , Ibadan , Nigeria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25166891

Citation

Owoeye, Olatunde, et al. "Modulatory Role of Kolaviron in Phenytoin-induced Hepatic and Testicular Dysfunctions in Wistar Rats." Journal of Dietary Supplements, vol. 12, no. 1, 2015, pp. 105-17.
Owoeye O, Adedara IA, Adeyemo OA, et al. Modulatory role of kolaviron in phenytoin-induced hepatic and testicular dysfunctions in Wistar rats. J Diet Suppl. 2015;12(1):105-17.
Owoeye, O., Adedara, I. A., Adeyemo, O. A., Bakare, O. S., Egun, C., & Farombi, E. O. (2015). Modulatory role of kolaviron in phenytoin-induced hepatic and testicular dysfunctions in Wistar rats. Journal of Dietary Supplements, 12(1), 105-17. https://doi.org/10.3109/19390211.2014.952862
Owoeye O, et al. Modulatory Role of Kolaviron in Phenytoin-induced Hepatic and Testicular Dysfunctions in Wistar Rats. J Diet Suppl. 2015;12(1):105-17. PubMed PMID: 25166891.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulatory role of kolaviron in phenytoin-induced hepatic and testicular dysfunctions in Wistar rats. AU - Owoeye,Olatunde, AU - Adedara,Isaac A, AU - Adeyemo,Oluwatobi A, AU - Bakare,Oluwafemi S, AU - Egun,Christa, AU - Farombi,Ebenezer O, Y1 - 2014/08/28/ PY - 2014/8/29/entrez PY - 2014/8/29/pubmed PY - 2015/9/24/medline KW - Kolaviron KW - Liver KW - Oxidative stress KW - Phenytoin KW - Testis KW - Vitamin E SP - 105 EP - 17 JF - Journal of dietary supplements JO - J Diet Suppl VL - 12 IS - 1 N2 - Phenytoin, an anticonvulsant agent used for the treatment of epilepsy has been reported to exhibit toxic side effects on the liver and testes. The present study investigated the protective effects of kolaviron (KV, a bioflavonoid from Garcinia kola seeds) against hepatic and testicular damage in rats exposed to phenytoin. The study consisted of four groups of six rats per group. Group I rats received 2 mL/kg of corn alone while group II received 75 mg/kg of phenytoin (PHT) alone. Groups III and IV were co-treated with kolaviron (200 mg/kg KV) and vitamin E (500 mg/kg VTE), respectively, for 14 days. The antioxidant status, hepatic and reproductive functional parameters were subsequently determined. PHT treatment significantly (p < 0.05) increased superoxide dismutase (SOD) and catalase (CAT) activities, elevated lipid peroxidation (LPO) and hydrogen peroxide (H2O2) levels along with significant reduction in the hepatic and testicular levels of glutathione (GSH). Moreover, PHT exposure elicited significant increases in alkaline phosphatase (ALP) and aspartate aminotransferase (AST) levels. The significant reduction in seminal epithelium thickness and the diameter of seminiferous tubules was accompanied with marked decrease in sperm motility, sperm count, and viability in PHT-treated rats. However, antioxidant status and the functional indices of liver and testes were restored to near control levels in rats co-treated with KV and VTE. In conclusion, KV and VTE protect the liver and testes against functional impairment due to PHT treatment. SN - 1939-022X UR - https://www.unboundmedicine.com/medline/citation/25166891/Modulatory_role_of_kolaviron_in_phenytoin_induced_hepatic_and_testicular_dysfunctions_in_Wistar_rats_ L2 - https://www.tandfonline.com/doi/full/10.3109/19390211.2014.952862 DB - PRIME DP - Unbound Medicine ER -