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Jungia sellowii suppresses the carrageenan-induced inflammatory response in the mouse model of pleurisy.
Inflammopharmacology. 2014 Dec; 22(6):351-65.I

Abstract

This study was conducted to explore the anti-inflammatory effect of Jungia sellowii (Asteraceae) using a murine model of pleurisy induced by carrageenan (Cg). This plant is used in southern Brazil to treat inflammatory diseases. J. sellowii leaves were extracted with ethanol/water to obtain the crude extract (CE), which was fractionated with different solvents, yielding n-hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol (BuOH) fractions, and aqueous fraction (Aq). The major compounds succinic acid (SA) and lactic acid (LA) were isolated from Aq fraction, and their structures were determined by (1)H and (13)C NMR. Pleurisy was induced by Cg (Saleh et al. 1996). The leukocytes, exudation, myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities, metabolites of nitric oxide (NO x) levels, protein levels and mRNA expression for interleukin 1 beta (IL-1β), tumour necrosis factor alpha (TNF-α), interleukin 17A (IL17A) and inducible of nitric oxide synthase (iNOs), and p65 protein phosphorylation (NF-κB) were analysed 4 h after pleurisy induction. Animals pre-treated with CE, BuOH, Aq, SA, or LA inhibited leukocytes, exudation, MPO and ADA activities, NO x , IL-1β, TNF-α, and IL-17A levels, and the mRNA expression for IL-1β, TNF-α, IL-17A, iNOS, and p65 protein phosphorylation (NF-κB) (p < 0.05). Our study demonstrated that J. sellowii can protect against inflammation induced by Cg by decreasing the leukocytes and exudation. Its effects are related to the decrease of either proinflammatory cytokines and/or NO x . The isolated compounds SA and LA may play an important role in this anti-inflammatory action by inhibiting all the studied parameters. The anti-inflammatory properties of these compounds are due to the downregulation of NF-κB.

Authors+Show Affiliations

Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina (UFSC), Campus Universitário-Trindade, Florianópolis, Santa Catarina, 88040-970, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25168307

Citation

Nader, Marina, et al. "Jungia Sellowii Suppresses the Carrageenan-induced Inflammatory Response in the Mouse Model of Pleurisy." Inflammopharmacology, vol. 22, no. 6, 2014, pp. 351-65.
Nader M, Vicente G, da Rosa JS, et al. Jungia sellowii suppresses the carrageenan-induced inflammatory response in the mouse model of pleurisy. Inflammopharmacology. 2014;22(6):351-65.
Nader, M., Vicente, G., da Rosa, J. S., Lima, T. C., Barbosa, A. M., Santos, A. D., Barison, A., Dalmarco, E. M., Biavatti, M. W., & Fröde, T. S. (2014). Jungia sellowii suppresses the carrageenan-induced inflammatory response in the mouse model of pleurisy. Inflammopharmacology, 22(6), 351-65. https://doi.org/10.1007/s10787-014-0210-3
Nader M, et al. Jungia Sellowii Suppresses the Carrageenan-induced Inflammatory Response in the Mouse Model of Pleurisy. Inflammopharmacology. 2014;22(6):351-65. PubMed PMID: 25168307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Jungia sellowii suppresses the carrageenan-induced inflammatory response in the mouse model of pleurisy. AU - Nader,Marina, AU - Vicente,Geison, AU - da Rosa,Julia Salvan, AU - Lima,Tamires Cardoso, AU - Barbosa,Alyne Machado, AU - Santos,Alan Diego Conceição, AU - Barison,Andersson, AU - Dalmarco,Eduardo Monguilhott, AU - Biavatti,Maique Weber, AU - Fröde,Tânia Silvia, Y1 - 2014/08/29/ PY - 2014/05/25/received PY - 2014/06/17/accepted PY - 2014/8/30/entrez PY - 2014/8/30/pubmed PY - 2015/7/7/medline SP - 351 EP - 65 JF - Inflammopharmacology JO - Inflammopharmacology VL - 22 IS - 6 N2 - This study was conducted to explore the anti-inflammatory effect of Jungia sellowii (Asteraceae) using a murine model of pleurisy induced by carrageenan (Cg). This plant is used in southern Brazil to treat inflammatory diseases. J. sellowii leaves were extracted with ethanol/water to obtain the crude extract (CE), which was fractionated with different solvents, yielding n-hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol (BuOH) fractions, and aqueous fraction (Aq). The major compounds succinic acid (SA) and lactic acid (LA) were isolated from Aq fraction, and their structures were determined by (1)H and (13)C NMR. Pleurisy was induced by Cg (Saleh et al. 1996). The leukocytes, exudation, myeloperoxidase (MPO) and adenosine-deaminase (ADA) activities, metabolites of nitric oxide (NO x) levels, protein levels and mRNA expression for interleukin 1 beta (IL-1β), tumour necrosis factor alpha (TNF-α), interleukin 17A (IL17A) and inducible of nitric oxide synthase (iNOs), and p65 protein phosphorylation (NF-κB) were analysed 4 h after pleurisy induction. Animals pre-treated with CE, BuOH, Aq, SA, or LA inhibited leukocytes, exudation, MPO and ADA activities, NO x , IL-1β, TNF-α, and IL-17A levels, and the mRNA expression for IL-1β, TNF-α, IL-17A, iNOS, and p65 protein phosphorylation (NF-κB) (p < 0.05). Our study demonstrated that J. sellowii can protect against inflammation induced by Cg by decreasing the leukocytes and exudation. Its effects are related to the decrease of either proinflammatory cytokines and/or NO x . The isolated compounds SA and LA may play an important role in this anti-inflammatory action by inhibiting all the studied parameters. The anti-inflammatory properties of these compounds are due to the downregulation of NF-κB. SN - 1568-5608 UR - https://www.unboundmedicine.com/medline/citation/25168307/Jungia_sellowii_suppresses_the_carrageenan_induced_inflammatory_response_in_the_mouse_model_of_pleurisy_ DB - PRIME DP - Unbound Medicine ER -