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Role of moxibustion in inflammatory responses during treatment of rat ulcerative colitis.
World J Gastroenterol. 2014 Aug 28; 20(32):11297-304.WJ

Abstract

AIM

To investigate the efficacy of moxibustion in ulcerative colitis (UC) rats from morphological, immunological and molecular biological perspectives.

METHODS

Thirty-two Sprague-Dawley rats were randomly assigned to a blank control group (normal rats, n = 6) and a model replication (MR) group (UC rats, n = 26). A UC model was established by 2,4,6-trinitrobenzenesulfonic acid/dextran sulfate sodium enema. Rats in the MR group were further randomly assigned to a 9-min moxibustion (9M) group (9 moxa-cone, n = 6), 6-min moxibustion (6M) group (6 moxa-cone, n = 6), 3-min moxibustion (3M) group (3 moxa-cone, n = 6), and a waiting list control (WLC) group (no moxibustion treatment, n = 6). Rats in the moxibustion treatment group were treated in 14 sessions over 28 d. Disease activity, local tissue morphology, serum level of interleukin (IL)-8 and IL-10, and expression of Toll-like receptor (TLR)9 as well as nuclear factor (NF)-κB p65 in colonic tissue were determined by disease activity index (DAI), hematoxylin and eosin staining, electron microscopy, enzyme-linked immunosorbent assay and Western blotting, respectively.

RESULTS

DAI was lowest in the 9M group and highest in the WLC group. The differences in DAI between the moxibustion treatment (3M, 6M, 9M) and no treatment groups were significant for all one-to-one comparisons (0.60 ± 0.54 vs 1.20 ± 0.44, 0.60 ± 0.54 vs 1.80 ± 0.45, 0.60 ± 0.54 vs 3.0 ± 0.45, respectively, P < 0.05). Light and electron microscopy showed that the neatness of the glandular arrangement in colonic mucosal epithelia gradually increased in the WLC, 3M, 6M to 9M groups. IL-8 level successively decreased while IL-10 level increased from the WLC to 3M, 6M and 9M groups. The differences among these groups were significant for all comparisons (105.46 ± 8.75 vs 76.61 ± 3.58, 105.46 ± 8.75 vs 69.78 ± 1.87, 105.46 ± 8.75 vs 67.41 ± 1.84, respectively, P < 0.01 for IL-8; and 30.83 ± 1.29 vs 75.64 ± 1.90, 30.83 ± 1.29 vs 80.90 ± 3.16, 30.83 ± 1.29 vs 83.46 ± 2.37, respectively, P < 0.01 for IL-10), except comparison of 6M vs 9M. Expression of TLR9 and NF-κB p65 decreased in order: highest in the WLC group and lowest in the 9M group. In addition, the differences among the WLC, 3M, 6M and 9M groups were significant for all comparisons (0.492 ± 0.026 vs 0.380 ± 0.022, 0.492 ± 0.026 vs 0.355 ± 0.005, 0.492 ± 0.026 vs 0.327 ± 0.015, respectively, P < 0.05 for TLR9; and 0.436 ± 0.041 vs 0.326 ± 0.022, 0.436 ± 0.041 vs 0.293 ± 0.006, 0.436 ± 0.041 vs 0.265 ± 0.017, respectively, P < 0.05 for NF-κB p65).

CONCLUSION

Moxibustion repairs damaged colonic mucosa, suppresses serum IL-8, activates serum IL-10 level, and decreases expression of TLR-9 and NF-κB p65 in UC rats.

Authors+Show Affiliations

Yang Han, Tie-Ming Ma, Lu Ren, Xian-De Ma, Zeng-Hua Bai, Department of Acupuncture and Massage, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China.Yang Han, Tie-Ming Ma, Lu Ren, Xian-De Ma, Zeng-Hua Bai, Department of Acupuncture and Massage, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China.Yang Han, Tie-Ming Ma, Lu Ren, Xian-De Ma, Zeng-Hua Bai, Department of Acupuncture and Massage, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China.Yang Han, Tie-Ming Ma, Lu Ren, Xian-De Ma, Zeng-Hua Bai, Department of Acupuncture and Massage, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China.Yang Han, Tie-Ming Ma, Lu Ren, Xian-De Ma, Zeng-Hua Bai, Department of Acupuncture and Massage, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China.Yang Han, Tie-Ming Ma, Lu Ren, Xian-De Ma, Zeng-Hua Bai, Department of Acupuncture and Massage, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25170214

Citation

Han, Yang, et al. "Role of Moxibustion in Inflammatory Responses During Treatment of Rat Ulcerative Colitis." World Journal of Gastroenterology, vol. 20, no. 32, 2014, pp. 11297-304.
Han Y, Ma TM, Lu ML, et al. Role of moxibustion in inflammatory responses during treatment of rat ulcerative colitis. World J Gastroenterol. 2014;20(32):11297-304.
Han, Y., Ma, T. M., Lu, M. L., Ren, L., Ma, X. D., & Bai, Z. H. (2014). Role of moxibustion in inflammatory responses during treatment of rat ulcerative colitis. World Journal of Gastroenterology, 20(32), 11297-304. https://doi.org/10.3748/wjg.v20.i32.11297
Han Y, et al. Role of Moxibustion in Inflammatory Responses During Treatment of Rat Ulcerative Colitis. World J Gastroenterol. 2014 Aug 28;20(32):11297-304. PubMed PMID: 25170214.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of moxibustion in inflammatory responses during treatment of rat ulcerative colitis. AU - Han,Yang, AU - Ma,Tie-Ming, AU - Lu,Mao-Lin, AU - Ren,Lu, AU - Ma,Xian-De, AU - Bai,Zeng-Hua, PY - 2014/03/20/received PY - 2014/05/30/revised PY - 2014/07/16/accepted PY - 2014/8/30/entrez PY - 2014/8/30/pubmed PY - 2015/5/16/medline KW - Disease activity index KW - Interleukin-10 KW - Interleukin-8 KW - Moxibustion KW - Nuclear factor-κB p65 KW - Toll-like receptor 9 KW - Ulcerative colitis SP - 11297 EP - 304 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 20 IS - 32 N2 - AIM: To investigate the efficacy of moxibustion in ulcerative colitis (UC) rats from morphological, immunological and molecular biological perspectives. METHODS: Thirty-two Sprague-Dawley rats were randomly assigned to a blank control group (normal rats, n = 6) and a model replication (MR) group (UC rats, n = 26). A UC model was established by 2,4,6-trinitrobenzenesulfonic acid/dextran sulfate sodium enema. Rats in the MR group were further randomly assigned to a 9-min moxibustion (9M) group (9 moxa-cone, n = 6), 6-min moxibustion (6M) group (6 moxa-cone, n = 6), 3-min moxibustion (3M) group (3 moxa-cone, n = 6), and a waiting list control (WLC) group (no moxibustion treatment, n = 6). Rats in the moxibustion treatment group were treated in 14 sessions over 28 d. Disease activity, local tissue morphology, serum level of interleukin (IL)-8 and IL-10, and expression of Toll-like receptor (TLR)9 as well as nuclear factor (NF)-κB p65 in colonic tissue were determined by disease activity index (DAI), hematoxylin and eosin staining, electron microscopy, enzyme-linked immunosorbent assay and Western blotting, respectively. RESULTS: DAI was lowest in the 9M group and highest in the WLC group. The differences in DAI between the moxibustion treatment (3M, 6M, 9M) and no treatment groups were significant for all one-to-one comparisons (0.60 ± 0.54 vs 1.20 ± 0.44, 0.60 ± 0.54 vs 1.80 ± 0.45, 0.60 ± 0.54 vs 3.0 ± 0.45, respectively, P < 0.05). Light and electron microscopy showed that the neatness of the glandular arrangement in colonic mucosal epithelia gradually increased in the WLC, 3M, 6M to 9M groups. IL-8 level successively decreased while IL-10 level increased from the WLC to 3M, 6M and 9M groups. The differences among these groups were significant for all comparisons (105.46 ± 8.75 vs 76.61 ± 3.58, 105.46 ± 8.75 vs 69.78 ± 1.87, 105.46 ± 8.75 vs 67.41 ± 1.84, respectively, P < 0.01 for IL-8; and 30.83 ± 1.29 vs 75.64 ± 1.90, 30.83 ± 1.29 vs 80.90 ± 3.16, 30.83 ± 1.29 vs 83.46 ± 2.37, respectively, P < 0.01 for IL-10), except comparison of 6M vs 9M. Expression of TLR9 and NF-κB p65 decreased in order: highest in the WLC group and lowest in the 9M group. In addition, the differences among the WLC, 3M, 6M and 9M groups were significant for all comparisons (0.492 ± 0.026 vs 0.380 ± 0.022, 0.492 ± 0.026 vs 0.355 ± 0.005, 0.492 ± 0.026 vs 0.327 ± 0.015, respectively, P < 0.05 for TLR9; and 0.436 ± 0.041 vs 0.326 ± 0.022, 0.436 ± 0.041 vs 0.293 ± 0.006, 0.436 ± 0.041 vs 0.265 ± 0.017, respectively, P < 0.05 for NF-κB p65). CONCLUSION: Moxibustion repairs damaged colonic mucosa, suppresses serum IL-8, activates serum IL-10 level, and decreases expression of TLR-9 and NF-κB p65 in UC rats. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/25170214/Role_of_moxibustion_in_inflammatory_responses_during_treatment_of_rat_ulcerative_colitis_ L2 - http://www.wjgnet.com/1007-9327/full/v20/i32/11297.htm DB - PRIME DP - Unbound Medicine ER -