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"Signal-on" photoelectrochemical sensing strategy based on target-dependent aptamer conformational conversion for selective detection of lead(II) ion.
ACS Appl Mater Interfaces. 2014 Sep 24; 6(18):15991-7.AA

Abstract

A "signal-on" photoelectrochemical sensing strategy for selective determination of Pb(2+) is designed on the basis of the combination of Pb(2+)-induced conformational conversion, the amplified effect of reduced graphene oxide (RGO) and resonance energy transfer between CdS quantum dots (QDs) and gold nanoparticles (AuNPs). The RGO/CdS/aptamer platform is constructed via a stepwise modification method, and characterized by electrochemical impedance spectroscopy. In the absence of Pb(2+), the AuNP-labeled DNA, as a signal quenching element, can be introduced by hybridization with aptamer on the surface of sensing platform, which quenches the photocurrent of QDs via an energy transfer process. Upon addition of Pb(2+), the aptamer is induced into a G-quadruplex structure, which can greatly hinder the hybridization between aptamer and AuNP-labeled DNA due to the competitive occupation of binding sites and steric effect, leading to the recovery of photocurrent. Under optimized conditions, this "signal-on" photoelectrochemical biosensor shows a linear relationship between photocurrent variation and the logarithm of Pb(2+) concentration in the range of 0.1-50 nM with a detection limit of 0.05 nM. Meanwhile, it also exhibits good selectivity for Pb(2+) over other interfering ions, and is successfully applied to the detection of Pb(2+) in environmental water samples. By substituting the aptamers with other sequences, this proposed strategy could be conveniently extended to detect different targets as versatile photoelectrochemical devices.

Authors+Show Affiliations

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25170538

Citation

Zang, Yang, et al. ""Signal-on" Photoelectrochemical Sensing Strategy Based On Target-dependent Aptamer Conformational Conversion for Selective Detection of lead(II) Ion." ACS Applied Materials & Interfaces, vol. 6, no. 18, 2014, pp. 15991-7.
Zang Y, Lei J, Hao Q, et al. "Signal-on" photoelectrochemical sensing strategy based on target-dependent aptamer conformational conversion for selective detection of lead(II) ion. ACS Appl Mater Interfaces. 2014;6(18):15991-7.
Zang, Y., Lei, J., Hao, Q., & Ju, H. (2014). "Signal-on" photoelectrochemical sensing strategy based on target-dependent aptamer conformational conversion for selective detection of lead(II) ion. ACS Applied Materials & Interfaces, 6(18), 15991-7. https://doi.org/10.1021/am503804g
Zang Y, et al. "Signal-on" Photoelectrochemical Sensing Strategy Based On Target-dependent Aptamer Conformational Conversion for Selective Detection of lead(II) Ion. ACS Appl Mater Interfaces. 2014 Sep 24;6(18):15991-7. PubMed PMID: 25170538.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - "Signal-on" photoelectrochemical sensing strategy based on target-dependent aptamer conformational conversion for selective detection of lead(II) ion. AU - Zang,Yang, AU - Lei,Jianping, AU - Hao,Qing, AU - Ju,Huangxian, Y1 - 2014/09/09/ PY - 2014/8/30/entrez PY - 2014/8/30/pubmed PY - 2015/10/22/medline KW - aptamer KW - biosensors KW - detection KW - photoelectrochemistry KW - quantum dots KW - resonance energy transfer SP - 15991 EP - 7 JF - ACS applied materials & interfaces JO - ACS Appl Mater Interfaces VL - 6 IS - 18 N2 - A "signal-on" photoelectrochemical sensing strategy for selective determination of Pb(2+) is designed on the basis of the combination of Pb(2+)-induced conformational conversion, the amplified effect of reduced graphene oxide (RGO) and resonance energy transfer between CdS quantum dots (QDs) and gold nanoparticles (AuNPs). The RGO/CdS/aptamer platform is constructed via a stepwise modification method, and characterized by electrochemical impedance spectroscopy. In the absence of Pb(2+), the AuNP-labeled DNA, as a signal quenching element, can be introduced by hybridization with aptamer on the surface of sensing platform, which quenches the photocurrent of QDs via an energy transfer process. Upon addition of Pb(2+), the aptamer is induced into a G-quadruplex structure, which can greatly hinder the hybridization between aptamer and AuNP-labeled DNA due to the competitive occupation of binding sites and steric effect, leading to the recovery of photocurrent. Under optimized conditions, this "signal-on" photoelectrochemical biosensor shows a linear relationship between photocurrent variation and the logarithm of Pb(2+) concentration in the range of 0.1-50 nM with a detection limit of 0.05 nM. Meanwhile, it also exhibits good selectivity for Pb(2+) over other interfering ions, and is successfully applied to the detection of Pb(2+) in environmental water samples. By substituting the aptamers with other sequences, this proposed strategy could be conveniently extended to detect different targets as versatile photoelectrochemical devices. SN - 1944-8252 UR - https://www.unboundmedicine.com/medline/citation/25170538/"Signal_on"_photoelectrochemical_sensing_strategy_based_on_target_dependent_aptamer_conformational_conversion_for_selective_detection_of_lead_II__ion_ L2 - https://doi.org/10.1021/am503804g DB - PRIME DP - Unbound Medicine ER -