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Basic leucine zipper family in barley: genome-wide characterization of members and expression analysis.
Mol Biotechnol. 2015 Jan; 57(1):12-26.MB

Abstract

The basic leucine zipper (bZIP) family is one of the largest and most diverse transcription factors in eukaryotes participating in many essential plant processes. We identified 141 bZIP proteins encoded by 89 genes from the Hordeum vulgare genome. HvbZIPs were classified into 11 groups based on their DNA-binding motif. Amino acid sequence alignment of the HvbZIPs basic-hinge regions revealed some highly conserved residues within each group. The leucine zipper heptads were analyzed predicting their dimerization properties. 34 conserved motifs were identified outside the bZIP domain. Phylogenetic analysis indicated that major diversification within the bZIP family predated the monocot/dicot divergence, although intra-species duplication and parallel evolution seems to be occurred afterward. Localization of HvbZIPs on the barley chromosomes revealed that different groups have been distributed on seven chromosomes of barley. Six types of intron pattern were detected within the basic-hinge regions. Most of the detected cis-elements in the promoter and UTR sequences were involved in seed development or abiotic stress response. Microarray data analysis revealed differential expression pattern of HvbZIPs in response to ABA treatment, drought, and cold stresses and during barley grain development and germination. This information would be helpful for functional characterization of bZIP transcription factors in barley.

Authors+Show Affiliations

Systems Biology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Seed and Plant Improvement Institutes Campus, Mahdasht Road, P. O. Box: 31535-1897, Karaj, Iran, e.pourabed@live.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25173685

Citation

Pourabed, Ehsan, et al. "Basic Leucine Zipper Family in Barley: Genome-wide Characterization of Members and Expression Analysis." Molecular Biotechnology, vol. 57, no. 1, 2015, pp. 12-26.
Pourabed E, Ghane Golmohamadi F, Soleymani Monfared P, et al. Basic leucine zipper family in barley: genome-wide characterization of members and expression analysis. Mol Biotechnol. 2015;57(1):12-26.
Pourabed, E., Ghane Golmohamadi, F., Soleymani Monfared, P., Razavi, S. M., & Shobbar, Z. S. (2015). Basic leucine zipper family in barley: genome-wide characterization of members and expression analysis. Molecular Biotechnology, 57(1), 12-26. https://doi.org/10.1007/s12033-014-9797-2
Pourabed E, et al. Basic Leucine Zipper Family in Barley: Genome-wide Characterization of Members and Expression Analysis. Mol Biotechnol. 2015;57(1):12-26. PubMed PMID: 25173685.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Basic leucine zipper family in barley: genome-wide characterization of members and expression analysis. AU - Pourabed,Ehsan, AU - Ghane Golmohamadi,Farzan, AU - Soleymani Monfared,Peyman, AU - Razavi,Seyed Morteza, AU - Shobbar,Zahra-Sadat, PY - 2014/9/1/entrez PY - 2014/9/1/pubmed PY - 2015/9/10/medline SP - 12 EP - 26 JF - Molecular biotechnology JO - Mol. Biotechnol. VL - 57 IS - 1 N2 - The basic leucine zipper (bZIP) family is one of the largest and most diverse transcription factors in eukaryotes participating in many essential plant processes. We identified 141 bZIP proteins encoded by 89 genes from the Hordeum vulgare genome. HvbZIPs were classified into 11 groups based on their DNA-binding motif. Amino acid sequence alignment of the HvbZIPs basic-hinge regions revealed some highly conserved residues within each group. The leucine zipper heptads were analyzed predicting their dimerization properties. 34 conserved motifs were identified outside the bZIP domain. Phylogenetic analysis indicated that major diversification within the bZIP family predated the monocot/dicot divergence, although intra-species duplication and parallel evolution seems to be occurred afterward. Localization of HvbZIPs on the barley chromosomes revealed that different groups have been distributed on seven chromosomes of barley. Six types of intron pattern were detected within the basic-hinge regions. Most of the detected cis-elements in the promoter and UTR sequences were involved in seed development or abiotic stress response. Microarray data analysis revealed differential expression pattern of HvbZIPs in response to ABA treatment, drought, and cold stresses and during barley grain development and germination. This information would be helpful for functional characterization of bZIP transcription factors in barley. SN - 1559-0305 UR - https://www.unboundmedicine.com/medline/citation/25173685/Basic_leucine_zipper_family_in_barley:_genome_wide_characterization_of_members_and_expression_analysis_ L2 - https://dx.doi.org/10.1007/s12033-014-9797-2 DB - PRIME DP - Unbound Medicine ER -