Tags

Type your tag names separated by a space and hit enter

PEG-liposomal oxaliplatin combined with nuclear factor-κB inhibitor (PDTC) induces apoptosis in human colorectal cancer cells.
Oncol Rep. 2014 Oct; 32(4):1617-21.OR

Abstract

To achieve sufficient antitumor activity for colorectal carcinoma, optimization of the therapeutic regimen is of great importance. The aim of the present study was to investigate the effects of polyethylene glycol (PEG)-liposomal oxaliplatin (L-OHP) on the induction of apoptosis in human colorectal cancer SW480 cells and how the nuclear factor-κB (NF-κB) pathway may contribute to mediating PEG-liposomal L-OHP-induced apoptosis. PEG-liposomal L-OHP was prepared and used to treat colorectal cancer SW480 cells. SW480 cell uptake of liposomes was observed by laser focus or SEM. Apoptosis was measured by flow cytometry (FCM) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay. Expression of NF-κB, Bcl-2, Bax and activated caspase-3 (P17) was examined by western blot analyses. The results indicated that PEG-liposomal L-OHP induced apoptosis. When pretreated with pyrrolidine dithiocarbamate (PDTC), PEG-liposomal L-OHP induced a significant apoptotic response. Moreover, apoptosis was associated with concentration of PDTC. Expression of protein p-P65, Bcl-2 was downregulated, but Bax and P17 were upregulated. These findings indicate that PEG-liposomal L-OHP enhances the anticancer potency of the chemotherapeutic agent. Moreover, NF-κB signaling pathways may contribute to mediating PEG-liposomal L-OHP-induced apoptosis.

Authors+Show Affiliations

Department of General Surgery, The Third People's Hospital of Mianyang, Mianyang, Sichuan 621000, P.R. China.Department of Gastrointestinal Surgery, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, P.R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25174808

Citation

Yang, Chuang, and Zhong-Xue Fu. "PEG-liposomal Oxaliplatin Combined With Nuclear factor-κB Inhibitor (PDTC) Induces Apoptosis in Human Colorectal Cancer Cells." Oncology Reports, vol. 32, no. 4, 2014, pp. 1617-21.
Yang C, Fu ZX. PEG-liposomal oxaliplatin combined with nuclear factor-κB inhibitor (PDTC) induces apoptosis in human colorectal cancer cells. Oncol Rep. 2014;32(4):1617-21.
Yang, C., & Fu, Z. X. (2014). PEG-liposomal oxaliplatin combined with nuclear factor-κB inhibitor (PDTC) induces apoptosis in human colorectal cancer cells. Oncology Reports, 32(4), 1617-21. https://doi.org/10.3892/or.2014.3336
Yang C, Fu ZX. PEG-liposomal Oxaliplatin Combined With Nuclear factor-κB Inhibitor (PDTC) Induces Apoptosis in Human Colorectal Cancer Cells. Oncol Rep. 2014;32(4):1617-21. PubMed PMID: 25174808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PEG-liposomal oxaliplatin combined with nuclear factor-κB inhibitor (PDTC) induces apoptosis in human colorectal cancer cells. AU - Yang,Chuang, AU - Fu,Zhong-Xue, Y1 - 2014/07/17/ PY - 2014/05/02/received PY - 2014/07/03/accepted PY - 2014/9/2/entrez PY - 2014/9/2/pubmed PY - 2015/6/2/medline SP - 1617 EP - 21 JF - Oncology reports JO - Oncol Rep VL - 32 IS - 4 N2 - To achieve sufficient antitumor activity for colorectal carcinoma, optimization of the therapeutic regimen is of great importance. The aim of the present study was to investigate the effects of polyethylene glycol (PEG)-liposomal oxaliplatin (L-OHP) on the induction of apoptosis in human colorectal cancer SW480 cells and how the nuclear factor-κB (NF-κB) pathway may contribute to mediating PEG-liposomal L-OHP-induced apoptosis. PEG-liposomal L-OHP was prepared and used to treat colorectal cancer SW480 cells. SW480 cell uptake of liposomes was observed by laser focus or SEM. Apoptosis was measured by flow cytometry (FCM) and terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay. Expression of NF-κB, Bcl-2, Bax and activated caspase-3 (P17) was examined by western blot analyses. The results indicated that PEG-liposomal L-OHP induced apoptosis. When pretreated with pyrrolidine dithiocarbamate (PDTC), PEG-liposomal L-OHP induced a significant apoptotic response. Moreover, apoptosis was associated with concentration of PDTC. Expression of protein p-P65, Bcl-2 was downregulated, but Bax and P17 were upregulated. These findings indicate that PEG-liposomal L-OHP enhances the anticancer potency of the chemotherapeutic agent. Moreover, NF-κB signaling pathways may contribute to mediating PEG-liposomal L-OHP-induced apoptosis. SN - 1791-2431 UR - https://www.unboundmedicine.com/medline/citation/25174808/PEG_liposomal_oxaliplatin_combined_with_nuclear_factor_κB_inhibitor__PDTC__induces_apoptosis_in_human_colorectal_cancer_cells_ L2 - http://www.spandidos-publications.com/or/32/4/1617 DB - PRIME DP - Unbound Medicine ER -