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Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus.
Biomed J. 2014 Nov-Dec; 37(6):411-4.BJ

Abstract

Although conventional antibiotic susceptibility tests are most commonly performed for methicillin-resistant Staphylococcus aureus (MRSA), the results of these phenotypic tests are dependent on the standardization of the culture conditions. The aim of the study was to evaluate the conventional phenotypic screening tests in comparison to the mecA gene polymerase chain reaction (PCR). One hundred and two clinical isolates of MRSA identified by the oxacillin disk diffusion were subjected to PCR for the mecA gene and by the cefoxitin disk diffusion test and culture on oxacillin screen agar, mannitol salt agar, and methicillin-resistant Staphylococcus aureus Agar (MeReSA) selective medium, for MRSA. Although all 102 isolates were resistant in oxacillin and cefoxitin disk diffusion, 92 (90.1%) isolates were positive for the mecA gene. The sensitivities of the mannitol salt agar, MeReSA agar, and oxacillin screen agar were 89.13, 97.82, and 98.91%, respectively. The oxacillin screen agar may be recommended for confirming methicillin resistance in the disk diffusion test in resource-poor settings, where molecular methods are not available.

Authors+Show Affiliations

Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Pondicherry, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25179712

Citation

Kali, Arunava, et al. "Laboratory Evaluation of Phenotypic Detection Methods of Methicillin-resistant Staphylococcus Aureus." Biomedical Journal, vol. 37, no. 6, 2014, pp. 411-4.
Kali A, Stephen S, Umadevi S. Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus. Biomed J. 2014;37(6):411-4.
Kali, A., Stephen, S., & Umadevi, S. (2014). Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus. Biomedical Journal, 37(6), 411-4. https://doi.org/10.4103/2319-4170.132907
Kali A, Stephen S, Umadevi S. Laboratory Evaluation of Phenotypic Detection Methods of Methicillin-resistant Staphylococcus Aureus. Biomed J. 2014;37(6):411-4. PubMed PMID: 25179712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus. AU - Kali,Arunava, AU - Stephen,Selvaraj, AU - Umadevi,Sivaraman, PY - 2014/9/3/entrez PY - 2014/9/3/pubmed PY - 2015/10/23/medline SP - 411 EP - 4 JF - Biomedical journal JO - Biomed J VL - 37 IS - 6 N2 - Although conventional antibiotic susceptibility tests are most commonly performed for methicillin-resistant Staphylococcus aureus (MRSA), the results of these phenotypic tests are dependent on the standardization of the culture conditions. The aim of the study was to evaluate the conventional phenotypic screening tests in comparison to the mecA gene polymerase chain reaction (PCR). One hundred and two clinical isolates of MRSA identified by the oxacillin disk diffusion were subjected to PCR for the mecA gene and by the cefoxitin disk diffusion test and culture on oxacillin screen agar, mannitol salt agar, and methicillin-resistant Staphylococcus aureus Agar (MeReSA) selective medium, for MRSA. Although all 102 isolates were resistant in oxacillin and cefoxitin disk diffusion, 92 (90.1%) isolates were positive for the mecA gene. The sensitivities of the mannitol salt agar, MeReSA agar, and oxacillin screen agar were 89.13, 97.82, and 98.91%, respectively. The oxacillin screen agar may be recommended for confirming methicillin resistance in the disk diffusion test in resource-poor settings, where molecular methods are not available. SN - 2320-2890 UR - https://www.unboundmedicine.com/medline/citation/25179712/Laboratory_evaluation_of_phenotypic_detection_methods_of_methicillin_resistant_Staphylococcus_aureus_ L2 - https://doi.org/10.4103/2319-4170.132907 DB - PRIME DP - Unbound Medicine ER -