Tags

Type your tag names separated by a space and hit enter

Edaravone protects human peripheral blood lymphocytes from γ-irradiation-induced apoptosis and DNA damage.
Cell Stress Chaperones. 2015 Mar; 20(2):289-95.CS

Abstract

Radiation-induced cellular injury is attributed primarily to the harmful effects of free radicals, which play a key role in irradiation-induced apoptosis. In this study, we investigated the radioprotective efficacy of edaravone, a licensed clinical drug and a powerful free radical scavenger that has been tested against γ-irradiation-induced cellular damage in cultured human peripheral blood lymphocytes in studies of various diseases. Edaravone was pre-incubated with lymphocytes for 2 h prior to γ-irradiation. It was found that pretreatment with edaravone increased cell viability and inhibited generation of γ-radiation-induced reactive oxygen species (ROS) in lymphocytes exposed to 3 Gy γ-radiation. In addition, γ-radiation decreased antioxidant enzymatic activity, such as superoxide dismutase and glutathione peroxidase, as well as the level of reduced glutathione. Conversely, treatment with 100 μM edaravone prior to irradiation improved antioxidant enzyme activity and increased reduced glutathione levels in irradiated lymphocytes. Importantly, we also report that edaravone reduced γ-irradiation-induced apoptosis through downregulation of Bax, upregulation of Bcl-2, and consequent reduction of the Bax:Bcl-2 ratio. The current study shows edaravone to be an effective radioprotector against γ-irradiation-induced cellular damage in lymphocytes in vitro. Finally, edaravone pretreatment significantly reduced DNA damage in γ-irradiated lymphocytes, as measured by comet assay (% tail DNA, tail length, tail moment, and olive tail moment) (p < 0.05). Thus, the current study indicates that edaravone offers protection from radiation-induced cytogenetic alterations.

Authors+Show Affiliations

Department of Hematology, Yantai Yuhuangding Hospital, No.20, Yuhuangding East Road, Yantai, Shandong Province, 264000, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25181965

Citation

Chen, Liming, et al. "Edaravone Protects Human Peripheral Blood Lymphocytes From Γ-irradiation-induced Apoptosis and DNA Damage." Cell Stress & Chaperones, vol. 20, no. 2, 2015, pp. 289-95.
Chen L, Liu Y, Dong L, et al. Edaravone protects human peripheral blood lymphocytes from γ-irradiation-induced apoptosis and DNA damage. Cell Stress Chaperones. 2015;20(2):289-95.
Chen, L., Liu, Y., Dong, L., & Chu, X. (2015). Edaravone protects human peripheral blood lymphocytes from γ-irradiation-induced apoptosis and DNA damage. Cell Stress & Chaperones, 20(2), 289-95. https://doi.org/10.1007/s12192-014-0542-3
Chen L, et al. Edaravone Protects Human Peripheral Blood Lymphocytes From Γ-irradiation-induced Apoptosis and DNA Damage. Cell Stress Chaperones. 2015;20(2):289-95. PubMed PMID: 25181965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Edaravone protects human peripheral blood lymphocytes from γ-irradiation-induced apoptosis and DNA damage. AU - Chen,Liming, AU - Liu,Yinghui, AU - Dong,Liangliang, AU - Chu,Xiaoxia, Y1 - 2014/09/03/ PY - 2014/06/10/received PY - 2014/08/21/accepted PY - 2014/08/20/revised PY - 2014/9/4/entrez PY - 2014/9/4/pubmed PY - 2015/11/11/medline SP - 289 EP - 95 JF - Cell stress & chaperones JO - Cell Stress Chaperones VL - 20 IS - 2 N2 - Radiation-induced cellular injury is attributed primarily to the harmful effects of free radicals, which play a key role in irradiation-induced apoptosis. In this study, we investigated the radioprotective efficacy of edaravone, a licensed clinical drug and a powerful free radical scavenger that has been tested against γ-irradiation-induced cellular damage in cultured human peripheral blood lymphocytes in studies of various diseases. Edaravone was pre-incubated with lymphocytes for 2 h prior to γ-irradiation. It was found that pretreatment with edaravone increased cell viability and inhibited generation of γ-radiation-induced reactive oxygen species (ROS) in lymphocytes exposed to 3 Gy γ-radiation. In addition, γ-radiation decreased antioxidant enzymatic activity, such as superoxide dismutase and glutathione peroxidase, as well as the level of reduced glutathione. Conversely, treatment with 100 μM edaravone prior to irradiation improved antioxidant enzyme activity and increased reduced glutathione levels in irradiated lymphocytes. Importantly, we also report that edaravone reduced γ-irradiation-induced apoptosis through downregulation of Bax, upregulation of Bcl-2, and consequent reduction of the Bax:Bcl-2 ratio. The current study shows edaravone to be an effective radioprotector against γ-irradiation-induced cellular damage in lymphocytes in vitro. Finally, edaravone pretreatment significantly reduced DNA damage in γ-irradiated lymphocytes, as measured by comet assay (% tail DNA, tail length, tail moment, and olive tail moment) (p < 0.05). Thus, the current study indicates that edaravone offers protection from radiation-induced cytogenetic alterations. SN - 1466-1268 UR - https://www.unboundmedicine.com/medline/citation/25181965/Edaravone_protects_human_peripheral_blood_lymphocytes_from_γ_irradiation_induced_apoptosis_and_DNA_damage_ L2 - https://dx.doi.org/10.1007/s12192-014-0542-3 DB - PRIME DP - Unbound Medicine ER -