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First isolation of Leptospira noguchii serogroups Panama and Autumnalis from cattle.
Epidemiol Infect 2015; 143(7):1538-41EI

Abstract

Prevention and control of leptospirosis are based on the knowledge of locally circulating strains. Thus, efforts to obtain local isolates are paramount to the epidemiological understanding of leptospirosis. We report and discuss here the first isolation of members of serogroups Autumnalis and Panama from cattle, both belonging to Leptospira noguchii species. Urine samples (n = 167) were collected directly by puncture of the bladder from randomly selected cows from a slaughterhouse in Rio de Janeiro, Brazil, for bacteriological culture. Isolates were characterized by serogrouping and sequencing (rrs and secY genes). Overall, 10/167 positive urine samples (6%) were obtained. Sequencing of amplicons targeting for both rrs and secY genes identified two of them (2013_U73 and 2013_U232) as L. noguchii. Serogrouping of those strains indicated that 2013_U73 belonged to the Panama serogroup (titre 1600), and 2013_U232 to the Autumnalis serogroup (titre 12800). Both Panama and Autumnalis are known agents of incidental leptospirosis in cattle. This group of leptospires could be particularly important in tropical countries. This is the first report of members of serogroups Autumnalis and Panama belonging to L. noguchii species from cattle. Although related to previously reported strains, these isolates have been shown to be genetically diverse from them.

Authors+Show Affiliations

Laboratório de Bacteriologia Veterinária,Departamento de Microbiologia e Parasitologia,Universidade Federal Fluminense,Niterói,Rio de Janeiro,Brazil.Laboratório de Bacteriologia Veterinária,Departamento de Microbiologia e Parasitologia,Universidade Federal Fluminense,Niterói,Rio de Janeiro,Brazil.No affiliation info availableLaboratório de Bacterioses,Departamento de Patologia Clínica,Universidade Federal da Bahia,Salvador,Bahia,Brazil.Institut Pasteur, Unité de Biologie des Spirochètes, National Reference Center and WHO Collaborating Center for Leptospirosis,Paris,France.Institut Pasteur, Unité de Biologie des Spirochètes, National Reference Center and WHO Collaborating Center for Leptospirosis,Paris,France.Laboratório de Bacteriologia Veterinária,Departamento de Microbiologia e Parasitologia,Universidade Federal Fluminense,Niterói,Rio de Janeiro,Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25185756

Citation

Martins, G, et al. "First Isolation of Leptospira Noguchii Serogroups Panama and Autumnalis From Cattle." Epidemiology and Infection, vol. 143, no. 7, 2015, pp. 1538-41.
Martins G, Loureiro AP, Hamond C, et al. First isolation of Leptospira noguchii serogroups Panama and Autumnalis from cattle. Epidemiol Infect. 2015;143(7):1538-41.
Martins, G., Loureiro, A. P., Hamond, C., Pinna, M. H., Bremont, S., Bourhy, P., & Lilenbaum, W. (2015). First isolation of Leptospira noguchii serogroups Panama and Autumnalis from cattle. Epidemiology and Infection, 143(7), pp. 1538-41. doi:10.1017/S0950268814002416.
Martins G, et al. First Isolation of Leptospira Noguchii Serogroups Panama and Autumnalis From Cattle. Epidemiol Infect. 2015;143(7):1538-41. PubMed PMID: 25185756.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - First isolation of Leptospira noguchii serogroups Panama and Autumnalis from cattle. AU - Martins,G, AU - Loureiro,A P, AU - Hamond,C, AU - Pinna,M H, AU - Bremont,S, AU - Bourhy,P, AU - Lilenbaum,W, Y1 - 2014/09/04/ PY - 2014/9/5/entrez PY - 2014/9/5/pubmed PY - 2015/6/3/medline KW - Panama SP - 1538 EP - 41 JF - Epidemiology and infection JO - Epidemiol. Infect. VL - 143 IS - 7 N2 - Prevention and control of leptospirosis are based on the knowledge of locally circulating strains. Thus, efforts to obtain local isolates are paramount to the epidemiological understanding of leptospirosis. We report and discuss here the first isolation of members of serogroups Autumnalis and Panama from cattle, both belonging to Leptospira noguchii species. Urine samples (n = 167) were collected directly by puncture of the bladder from randomly selected cows from a slaughterhouse in Rio de Janeiro, Brazil, for bacteriological culture. Isolates were characterized by serogrouping and sequencing (rrs and secY genes). Overall, 10/167 positive urine samples (6%) were obtained. Sequencing of amplicons targeting for both rrs and secY genes identified two of them (2013_U73 and 2013_U232) as L. noguchii. Serogrouping of those strains indicated that 2013_U73 belonged to the Panama serogroup (titre 1600), and 2013_U232 to the Autumnalis serogroup (titre 12800). Both Panama and Autumnalis are known agents of incidental leptospirosis in cattle. This group of leptospires could be particularly important in tropical countries. This is the first report of members of serogroups Autumnalis and Panama belonging to L. noguchii species from cattle. Although related to previously reported strains, these isolates have been shown to be genetically diverse from them. SN - 1469-4409 UR - https://www.unboundmedicine.com/medline/citation/25185756/First_isolation_of_Leptospira_noguchii_serogroups_Panama_and_Autumnalis_from_cattle_ L2 - https://www.cambridge.org/core/product/identifier/S0950268814002416/type/journal_article DB - PRIME DP - Unbound Medicine ER -