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Identification and characterisation of phenolics from Ixora coccinea L. (Rubiaceae) by liquid chromatography multi-stage mass spectrometry.
Phytochem Anal. 2014 Nov-Dec; 25(6):567-76.PA

Abstract

INTRODUCTION

Ixora coccinea L. leaves and stem are used in traditional Sudanese and Ayurvedic medicinal systems for the treatment of diarrhoea, fever, headache, skin diseases, eye trouble, wounds, sores and ulcers. Recent studies show that I. coccinea has anti-oxidant, anti-bacterial, anti-cancer, anti-inflammatory, analgaesic, anti-diarrhoeal, hepatoprotective, cardioprotective, anti-mutagenic, wound healing and anti-tumour activities. Ixora coccinea is a rich source of polyphenols such as proanthocyanidins, flavonoids, flavonoids glycosides and tannins.

OBJECTIVES

To develop a LC-MS(n) method for the identification and characterisation of phenolic compounds of I. coccinea L. leaves and stem.

METHODS

Aqueous methanolic (70% methanol) extracts of I. coccinea leaves and stem were used for LC-MS(n) to ensure efficient extraction of phenolics. A C18 amide reverse-phase HPLC column allowed separation of the phenolic compounds, including different isomers. For the LC-MS measurements, negative ion mode was used in order to obtain better tandem mass spectra and high-resolution mass spectra.

RESULTS

The phenolics were identified by their typical UV absorptions at 254, 280 and 320 nm. All the flavonol glycosides showed a neutral loss of the glycan part; hydroxycinnamates showed loss of the cinnamoyl/cinnamic acid part; while proanthocyanidins showed a Diels-Alder fragment in negative ion mode mass spectra.

CONCLUSION

It was possible to identify C-3 and C-7 flavonol glycosides by their order of elution and it was also possible to predict the glycosylation position in flavonol diglycosides from their tandem mass spectra.

Authors+Show Affiliations

School of Engineering and Science, Chemistry, Jacobs University Bremen, Campus Ring 8, 28759, Bremen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25185927

Citation

Jaiswal, Rakesh, et al. "Identification and Characterisation of Phenolics From Ixora Coccinea L. (Rubiaceae) By Liquid Chromatography Multi-stage Mass Spectrometry." Phytochemical Analysis : PCA, vol. 25, no. 6, 2014, pp. 567-76.
Jaiswal R, Karar MG, Gadir HA, et al. Identification and characterisation of phenolics from Ixora coccinea L. (Rubiaceae) by liquid chromatography multi-stage mass spectrometry. Phytochem Anal. 2014;25(6):567-76.
Jaiswal, R., Karar, M. G., Gadir, H. A., & Kuhnert, N. (2014). Identification and characterisation of phenolics from Ixora coccinea L. (Rubiaceae) by liquid chromatography multi-stage mass spectrometry. Phytochemical Analysis : PCA, 25(6), 567-76. https://doi.org/10.1002/pca.2530
Jaiswal R, et al. Identification and Characterisation of Phenolics From Ixora Coccinea L. (Rubiaceae) By Liquid Chromatography Multi-stage Mass Spectrometry. Phytochem Anal. 2014 Nov-Dec;25(6):567-76. PubMed PMID: 25185927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterisation of phenolics from Ixora coccinea L. (Rubiaceae) by liquid chromatography multi-stage mass spectrometry. AU - Jaiswal,Rakesh, AU - Karar,Mohamed Gamaleldin Elsadig, AU - Gadir,Haidar Abdel, AU - Kuhnert,Nikolai, Y1 - 2014/09/04/ PY - 2013/12/29/received PY - 2014/05/22/revised PY - 2014/05/30/accepted PY - 2014/9/5/entrez PY - 2014/9/5/pubmed PY - 2015/8/19/medline KW - Ixora coccinea L KW - Rubiaceae KW - Tandem MS KW - flavonoid glycosides KW - phenolics KW - polyphenols KW - proanthocyanidins SP - 567 EP - 76 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 25 IS - 6 N2 - INTRODUCTION: Ixora coccinea L. leaves and stem are used in traditional Sudanese and Ayurvedic medicinal systems for the treatment of diarrhoea, fever, headache, skin diseases, eye trouble, wounds, sores and ulcers. Recent studies show that I. coccinea has anti-oxidant, anti-bacterial, anti-cancer, anti-inflammatory, analgaesic, anti-diarrhoeal, hepatoprotective, cardioprotective, anti-mutagenic, wound healing and anti-tumour activities. Ixora coccinea is a rich source of polyphenols such as proanthocyanidins, flavonoids, flavonoids glycosides and tannins. OBJECTIVES: To develop a LC-MS(n) method for the identification and characterisation of phenolic compounds of I. coccinea L. leaves and stem. METHODS: Aqueous methanolic (70% methanol) extracts of I. coccinea leaves and stem were used for LC-MS(n) to ensure efficient extraction of phenolics. A C18 amide reverse-phase HPLC column allowed separation of the phenolic compounds, including different isomers. For the LC-MS measurements, negative ion mode was used in order to obtain better tandem mass spectra and high-resolution mass spectra. RESULTS: The phenolics were identified by their typical UV absorptions at 254, 280 and 320 nm. All the flavonol glycosides showed a neutral loss of the glycan part; hydroxycinnamates showed loss of the cinnamoyl/cinnamic acid part; while proanthocyanidins showed a Diels-Alder fragment in negative ion mode mass spectra. CONCLUSION: It was possible to identify C-3 and C-7 flavonol glycosides by their order of elution and it was also possible to predict the glycosylation position in flavonol diglycosides from their tandem mass spectra. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/25185927/Identification_and_characterisation_of_phenolics_from_Ixora_coccinea_L___Rubiaceae__by_liquid_chromatography_multi_stage_mass_spectrometry_ L2 - https://doi.org/10.1002/pca.2530 DB - PRIME DP - Unbound Medicine ER -