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Quantitative analysis of afferents expressing substance P, calcitonin gene-related peptide, isolectin B4, neurofilament 200, and Peripherin in the sensory root of the rat trigeminal ganglion.
J Comp Neurol. 2015 Jan 01; 523(1):126-38.JC

Abstract

Substance P (SP), calcitonin gene-related peptide (CGRP), and isolectin B4 (IB4) are widely used as markers for peripheral neurons with unmyelinated fibers, whereas neurofilament 200 (NF200), and Peripherin are used as markers for neurons with myelinated fibers, and with unmyelinated or small-caliber fibers, respectively. To study the selectivity of these markers for specific neuronal types, we analyzed their expression in neurons in the rat trigeminal ganglion by light- and electron-microscopic immunocytochemistry. Most SP-immunopositive (+), CGRP+, and IB4+ fibers were unmyelinated, but a small fraction (∼5%) were small myelinated fibers (<20 µm(2) in cross-sectional area, equivalent to <5 µm in diameter, Aδ fiber). Similarly, whereas the majority of NF200+ fibers were myelinated, a large fraction (23.9%) were unmyelinated, and whereas the majority of Peripherin+ fibers were unmyelinated and small myelinated, a significant fraction (15.5%) were large myelinated (>20 µm(2) in cross-sectional area, equivalent to >5 µm in diameter, Aβ fiber). Our findings confirm that SP, CGRP, and IB4 can be used as reliable markers for neurons with unmyelinated fibers, and question the suitability of NF200 as a marker for neurons with myelinated fibers, and of Peripherin as a marker for neurons with unmyelinated, or fine-caliber fibers.

Authors+Show Affiliations

Department of Anatomy and Neurobiology, School of Dentistry, Kyungpook National University, Daegu, 700-412, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25185935

Citation

Bae, Jin Young, et al. "Quantitative Analysis of Afferents Expressing Substance P, Calcitonin Gene-related Peptide, Isolectin B4, Neurofilament 200, and Peripherin in the Sensory Root of the Rat Trigeminal Ganglion." The Journal of Comparative Neurology, vol. 523, no. 1, 2015, pp. 126-38.
Bae JY, Kim JH, Cho YS, et al. Quantitative analysis of afferents expressing substance P, calcitonin gene-related peptide, isolectin B4, neurofilament 200, and Peripherin in the sensory root of the rat trigeminal ganglion. J Comp Neurol. 2015;523(1):126-38.
Bae, J. Y., Kim, J. H., Cho, Y. S., Mah, W., & Bae, Y. C. (2015). Quantitative analysis of afferents expressing substance P, calcitonin gene-related peptide, isolectin B4, neurofilament 200, and Peripherin in the sensory root of the rat trigeminal ganglion. The Journal of Comparative Neurology, 523(1), 126-38. https://doi.org/10.1002/cne.23672
Bae JY, et al. Quantitative Analysis of Afferents Expressing Substance P, Calcitonin Gene-related Peptide, Isolectin B4, Neurofilament 200, and Peripherin in the Sensory Root of the Rat Trigeminal Ganglion. J Comp Neurol. 2015 Jan 1;523(1):126-38. PubMed PMID: 25185935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative analysis of afferents expressing substance P, calcitonin gene-related peptide, isolectin B4, neurofilament 200, and Peripherin in the sensory root of the rat trigeminal ganglion. AU - Bae,Jin Young, AU - Kim,Jae Hyun, AU - Cho,Yi Sul, AU - Mah,Won, AU - Bae,Yong Chul, PY - 2014/06/03/received PY - 2014/08/21/revised PY - 2014/08/25/accepted PY - 2014/9/5/entrez PY - 2014/9/5/pubmed PY - 2015/7/22/medline KW - AB_2171328 KW - AB_2314660 KW - AB_477257 KW - AB_572217 KW - AB_572266 KW - RGD_1566440 KW - fiber type KW - neuronal marker KW - nif-0000-30467 KW - sensory root KW - trigeminal ganglion KW - ultrastructure SP - 126 EP - 38 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 523 IS - 1 N2 - Substance P (SP), calcitonin gene-related peptide (CGRP), and isolectin B4 (IB4) are widely used as markers for peripheral neurons with unmyelinated fibers, whereas neurofilament 200 (NF200), and Peripherin are used as markers for neurons with myelinated fibers, and with unmyelinated or small-caliber fibers, respectively. To study the selectivity of these markers for specific neuronal types, we analyzed their expression in neurons in the rat trigeminal ganglion by light- and electron-microscopic immunocytochemistry. Most SP-immunopositive (+), CGRP+, and IB4+ fibers were unmyelinated, but a small fraction (∼5%) were small myelinated fibers (<20 µm(2) in cross-sectional area, equivalent to <5 µm in diameter, Aδ fiber). Similarly, whereas the majority of NF200+ fibers were myelinated, a large fraction (23.9%) were unmyelinated, and whereas the majority of Peripherin+ fibers were unmyelinated and small myelinated, a significant fraction (15.5%) were large myelinated (>20 µm(2) in cross-sectional area, equivalent to >5 µm in diameter, Aβ fiber). Our findings confirm that SP, CGRP, and IB4 can be used as reliable markers for neurons with unmyelinated fibers, and question the suitability of NF200 as a marker for neurons with myelinated fibers, and of Peripherin as a marker for neurons with unmyelinated, or fine-caliber fibers. SN - 1096-9861 UR - https://www.unboundmedicine.com/medline/citation/25185935/Quantitative_analysis_of_afferents_expressing_substance_P_calcitonin_gene_related_peptide_isolectin_B4_neurofilament_200_and_Peripherin_in_the_sensory_root_of_the_rat_trigeminal_ganglion_ L2 - https://doi.org/10.1002/cne.23672 DB - PRIME DP - Unbound Medicine ER -