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Determination of phenolic acids and flavonoids in raw propolis by silica-supported ionic liquid-based matrix solid phase dispersion extraction high performance liquid chromatography-diode array detection.

Abstract

The silica-supported ionic liquid (S-SIL) was prepared by impregnation and used as the dispersion adsorbent of matrix solid phase dispersion (MSPD) for the simultaneous extraction of eight phenolic acids and flavonoids, including caffeic acid, ferulic acid, morin, luteolin, quercetin, apigenin, chrysin, and kaempferide in raw propolis. High performance liquid chromatography with a Zorbax SB-C18 column (150mm×4.6mm, 3.5μm) was used for separation of the analytes. The mobile phase consisted of 0.2% phosphoric acid aqueous solution and acetonitrile and the flow rate of the mobile phase was 0.5mL/min. The experimental conditions for silica-supported ionic liquid-based matrix solid phase dispersion (S-SIL-based MSPD) were optimized. S-SIL containing 10% [C6MIM]Cl was used as dispersant, 20mL of n-hexane as washing solvent and 15mL of methanol as elution solvent. The ratio of S-SIL to sample was selected to be 4:1. The standard curves showed good linear relationship (r>0.9995). The limits of detection and quantification were in the range of 5.8-22.2ngmL(-1) and 19.2-74.0ngmL(-1), respectively. The relative standard deviations (RSDs) of intra-day and inter-day determination were lower than 8.80% and 11.19%, respectively. The recoveries were between 65.51% and 92.32% with RSDs lower than 8.95%. Compared with ultrasound-assisted extraction (UAE) and soxhlet extraction, the present method consumed less sample, organic solvent, and extraction time, although the extraction yields obtained by S-SIL-based MSPD are slightly lower than those obtained by UAE.

Authors+Show Affiliations

College of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China; College of Chemistry, Jilin University, Changchun 130012, China.College of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China; School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.College of Chemistry, Jilin University, Changchun 130012, China.College of Chemistry, Jilin University, Changchun 130012, China.College of Chemistry, Jilin University, Changchun 130012, China.College of Chemistry, Jilin University, Changchun 130012, China.College of Chemistry, Jilin University, Changchun 130012, China.College of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China. Electronic address: analchem@jlu.edu.cn.College of Chemistry, Jilin University, Changchun 130012, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25190186

Citation

Wang, Zhibing, et al. "Determination of Phenolic Acids and Flavonoids in Raw Propolis By Silica-supported Ionic Liquid-based Matrix Solid Phase Dispersion Extraction High Performance Liquid Chromatography-diode Array Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 969, 2014, pp. 205-12.
Wang Z, Sun R, Wang Y, et al. Determination of phenolic acids and flavonoids in raw propolis by silica-supported ionic liquid-based matrix solid phase dispersion extraction high performance liquid chromatography-diode array detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2014;969:205-12.
Wang, Z., Sun, R., Wang, Y., Li, N., Lei, L., Yang, X., Yu, A., Qiu, F., & Zhang, H. (2014). Determination of phenolic acids and flavonoids in raw propolis by silica-supported ionic liquid-based matrix solid phase dispersion extraction high performance liquid chromatography-diode array detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 969, 205-12. https://doi.org/10.1016/j.jchromb.2014.08.022
Wang Z, et al. Determination of Phenolic Acids and Flavonoids in Raw Propolis By Silica-supported Ionic Liquid-based Matrix Solid Phase Dispersion Extraction High Performance Liquid Chromatography-diode Array Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Oct 15;969:205-12. PubMed PMID: 25190186.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of phenolic acids and flavonoids in raw propolis by silica-supported ionic liquid-based matrix solid phase dispersion extraction high performance liquid chromatography-diode array detection. AU - Wang,Zhibing, AU - Sun,Rui, AU - Wang,Yuanpeng, AU - Li,Na, AU - Lei,Lei, AU - Yang,Xiao, AU - Yu,Aimin, AU - Qiu,Fangping, AU - Zhang,Hanqi, Y1 - 2014/08/27/ PY - 2014/06/19/received PY - 2014/08/14/revised PY - 2014/08/17/accepted PY - 2014/9/6/entrez PY - 2014/9/6/pubmed PY - 2015/5/13/medline KW - High-performance liquid chromatography (HPLC) KW - Phenolic acids and flavonoids KW - Raw propolis KW - Silica-supported ionic liquid-based matrix solid phase dispersion (S-SIL-based MSPD) SP - 205 EP - 12 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 969 N2 - The silica-supported ionic liquid (S-SIL) was prepared by impregnation and used as the dispersion adsorbent of matrix solid phase dispersion (MSPD) for the simultaneous extraction of eight phenolic acids and flavonoids, including caffeic acid, ferulic acid, morin, luteolin, quercetin, apigenin, chrysin, and kaempferide in raw propolis. High performance liquid chromatography with a Zorbax SB-C18 column (150mm×4.6mm, 3.5μm) was used for separation of the analytes. The mobile phase consisted of 0.2% phosphoric acid aqueous solution and acetonitrile and the flow rate of the mobile phase was 0.5mL/min. The experimental conditions for silica-supported ionic liquid-based matrix solid phase dispersion (S-SIL-based MSPD) were optimized. S-SIL containing 10% [C6MIM]Cl was used as dispersant, 20mL of n-hexane as washing solvent and 15mL of methanol as elution solvent. The ratio of S-SIL to sample was selected to be 4:1. The standard curves showed good linear relationship (r>0.9995). The limits of detection and quantification were in the range of 5.8-22.2ngmL(-1) and 19.2-74.0ngmL(-1), respectively. The relative standard deviations (RSDs) of intra-day and inter-day determination were lower than 8.80% and 11.19%, respectively. The recoveries were between 65.51% and 92.32% with RSDs lower than 8.95%. Compared with ultrasound-assisted extraction (UAE) and soxhlet extraction, the present method consumed less sample, organic solvent, and extraction time, although the extraction yields obtained by S-SIL-based MSPD are slightly lower than those obtained by UAE. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/25190186/Determination_of_phenolic_acids_and_flavonoids_in_raw_propolis_by_silica_supported_ionic_liquid_based_matrix_solid_phase_dispersion_extraction_high_performance_liquid_chromatography_diode_array_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(14)00539-X DB - PRIME DP - Unbound Medicine ER -