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Detection of immunoglobulin IgA and IgG against human papilloma virus.
Viral Immunol. 2014 Nov; 27(9):471-7.VI

Abstract

The interest in human papilloma virus (HPV) seropositivity has increased considerably since HPV vaccines have become available worldwide. The aim of this study was to assess the performance of enzyme-linked immunosorbent assay (ELISA) in analyzing serum samples provided from women with and without genital DNA-HPV infection confirmed by polymerase chain reaction (PCR), for detection of specific antibodies of the isotypes IgG and IgA recognizing HPV-16 and -18, as well as virus-like particles (VLPs). From August to December 2013, 50 sexually active female patients between 18 and 35 years of age from the outpatient clinic at the university hospital were enrolled. In order to test them, positive controls were obtained from patients with HPV-induced lesions and who were DNA-HPV positive confirmed by PCR. A specific assay was used to identify antibodies to HPV VLPs by ELISA. The samples were divided into HPV positive and negative, and an ELISA detecting IgA and IgG anti-HPV-VLP was carried out. The effectiveness of ELISA and the kappa (k) index was obtained from the values entered in the receiver operating characteristic (ROC) curves for IgG and IgA. IgG-VLP-HPV-16 showed a good correlation between ELISA and PCR (k=0.75), and IgG-VLP-HPV-18 showed a very good correlation between ELISA and PCR (k=0.84). While the IgA antibody correlation was also positive, although weaker, IgA-VLP-HPV-16 was moderate (k=0.45) and IgA-VLP-HPV-18 good (k=0.66). The efficacy of the assay concerning IgG was: sensitivity, specificity, and accuracy were 82.3%, 92%, and 88% to IgG-VLP-HPV-16, and 100%, 92%, and 94% to IgG-VLP-HPV-18. The assay concerning IgA was: sensitivity, specificity, and accuracy were 64.7%, 80%, and 73.8% to IgA-VLP-HPV-16, and 100%, 80%, and 84.8% to IgA-VLP-HPV-18. IgG and IgA antibodies against HPV-16 and -18 can be detected in unvaccinated individuals by using the VLP that serve as the basis for bivalent HPV vaccine. The values for ELISA assays and the values found for IgG correlate good/very good with HPV-16/18 detected by PCR.

Authors+Show Affiliations

1 Department of Obstetrics and Gynecology, Universidade Federal do Rio Grande do Norte , Natal-RN, Brazil .No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25191973

Citation

Gonçalves, Ana Katherine, et al. "Detection of Immunoglobulin IgA and IgG Against Human Papilloma Virus." Viral Immunology, vol. 27, no. 9, 2014, pp. 471-7.
Gonçalves AK, Machado PR, de Souza LC, et al. Detection of immunoglobulin IgA and IgG against human papilloma virus. Viral Immunol. 2014;27(9):471-7.
Gonçalves, A. K., Machado, P. R., de Souza, L. C., Costa, A. P., Gimenes, F., Consolaro, M. L., Crispim, J. O., Eleutério, J., & Giraldo, P. C. (2014). Detection of immunoglobulin IgA and IgG against human papilloma virus. Viral Immunology, 27(9), 471-7. https://doi.org/10.1089/vim.2014.0033
Gonçalves AK, et al. Detection of Immunoglobulin IgA and IgG Against Human Papilloma Virus. Viral Immunol. 2014;27(9):471-7. PubMed PMID: 25191973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of immunoglobulin IgA and IgG against human papilloma virus. AU - Gonçalves,Ana Katherine, AU - Machado,Paula Renata Lima, AU - de Souza,Luanda Canário, AU - Costa,Ana Paula Ferreira, AU - Gimenes,Fabrícia, AU - Consolaro,Marcia Lopes, AU - Crispim,Janaina Oliveira, AU - Eleutério,Jose,Jr AU - Giraldo,Paulo César, Y1 - 2014/09/05/ PY - 2014/9/6/entrez PY - 2014/9/6/pubmed PY - 2015/6/24/medline SP - 471 EP - 7 JF - Viral immunology JO - Viral Immunol VL - 27 IS - 9 N2 - The interest in human papilloma virus (HPV) seropositivity has increased considerably since HPV vaccines have become available worldwide. The aim of this study was to assess the performance of enzyme-linked immunosorbent assay (ELISA) in analyzing serum samples provided from women with and without genital DNA-HPV infection confirmed by polymerase chain reaction (PCR), for detection of specific antibodies of the isotypes IgG and IgA recognizing HPV-16 and -18, as well as virus-like particles (VLPs). From August to December 2013, 50 sexually active female patients between 18 and 35 years of age from the outpatient clinic at the university hospital were enrolled. In order to test them, positive controls were obtained from patients with HPV-induced lesions and who were DNA-HPV positive confirmed by PCR. A specific assay was used to identify antibodies to HPV VLPs by ELISA. The samples were divided into HPV positive and negative, and an ELISA detecting IgA and IgG anti-HPV-VLP was carried out. The effectiveness of ELISA and the kappa (k) index was obtained from the values entered in the receiver operating characteristic (ROC) curves for IgG and IgA. IgG-VLP-HPV-16 showed a good correlation between ELISA and PCR (k=0.75), and IgG-VLP-HPV-18 showed a very good correlation between ELISA and PCR (k=0.84). While the IgA antibody correlation was also positive, although weaker, IgA-VLP-HPV-16 was moderate (k=0.45) and IgA-VLP-HPV-18 good (k=0.66). The efficacy of the assay concerning IgG was: sensitivity, specificity, and accuracy were 82.3%, 92%, and 88% to IgG-VLP-HPV-16, and 100%, 92%, and 94% to IgG-VLP-HPV-18. The assay concerning IgA was: sensitivity, specificity, and accuracy were 64.7%, 80%, and 73.8% to IgA-VLP-HPV-16, and 100%, 80%, and 84.8% to IgA-VLP-HPV-18. IgG and IgA antibodies against HPV-16 and -18 can be detected in unvaccinated individuals by using the VLP that serve as the basis for bivalent HPV vaccine. The values for ELISA assays and the values found for IgG correlate good/very good with HPV-16/18 detected by PCR. SN - 1557-8976 UR - https://www.unboundmedicine.com/medline/citation/25191973/Detection_of_immunoglobulin_IgA_and_IgG_against_human_papilloma_virus_ L2 - https://www.liebertpub.com/doi/10.1089/vim.2014.0033?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -