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Diversity in gut microflora of Helicoverpa armigera populations from different regions in relation to biological activity of Bacillus thuringiensis δ-endotoxin Cry1Ac.
Arch Insect Biochem Physiol. 2014 Dec; 87(4):201-13.AI

Abstract

Transgenic crops expressing toxin proteins from Bacillus thuringiensis (Bt) have been deployed on a large scale for management of Helicoverpa armigera. Resistance to Bt toxins has been documented in several papers, and therefore, we examined the role of midgut microflora of H. armigera in its susceptibility to Bt toxins. The susceptibility of H. armigera to Bt toxin Cry1Ac was assessed using Log-dose-Probit analysis, and the microbial communities were identified by 16S rRNA sequencing. The H. armigera populations from nine locations harbored diverse microbial communities, and had some unique bacteria, suggesting a wide geographical variation in microbial community in the midgut of the pod borer larvae. Phylotypes belonging to 32 genera were identified in the H. armigera midgut in field populations from nine locations. Bacteria belonging to Enterobacteriaceae (Order Bacillales) were present in all the populations, and these may be the common members of the H. armigera larval midgut microflora. Presence and/or absence of certain species were linked to H. armigera susceptibility to Bt toxins, but there were no clear trends across locations. Variation in susceptibility of F1 neonates of H. armigera from different locations to the Bt toxin Cry1Ac was found to be 3.4-fold. These findings support the idea that insect migut microflora may influence the biological activity of Bt toxins.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Paramasiva I
Department of Entomology, Agricultural College, Acharya N.G. Ranga Agriculture University, Bapatla, Andhra Pradesh, India; Entomology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Telangana, India.
Shouche Y
No affiliation info available
Kulkarni GJ
No affiliation info available
Krishnayya PV
No affiliation info available
Akbar SM
No affiliation info available
Sharma HC
No affiliation info available

MeSH

AnimalsBacillus thuringiensisBacillus thuringiensis ToxinsBacterial ProteinsBacterial ToxinsDigestive SystemEndotoxinsEnterobacteriaceaeHemolysin ProteinsIndiaInsecticide ResistanceLarvaMothsPest Control, BiologicalRNA, Ribosomal, 16S

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25195523

Citation

Paramasiva, Inakarla, et al. "Diversity in Gut Microflora of Helicoverpa Armigera Populations From Different Regions in Relation to Biological Activity of Bacillus Thuringiensis Δ-endotoxin Cry1Ac." Archives of Insect Biochemistry and Physiology, vol. 87, no. 4, 2014, pp. 201-13.
Paramasiva I, Shouche Y, Kulkarni GJ, et al. Diversity in gut microflora of Helicoverpa armigera populations from different regions in relation to biological activity of Bacillus thuringiensis δ-endotoxin Cry1Ac. Arch Insect Biochem Physiol. 2014;87(4):201-13.
Paramasiva, I., Shouche, Y., Kulkarni, G. J., Krishnayya, P. V., Akbar, S. M., & Sharma, H. C. (2014). Diversity in gut microflora of Helicoverpa armigera populations from different regions in relation to biological activity of Bacillus thuringiensis δ-endotoxin Cry1Ac. Archives of Insect Biochemistry and Physiology, 87(4), 201-13. https://doi.org/10.1002/arch.21190
Paramasiva I, et al. Diversity in Gut Microflora of Helicoverpa Armigera Populations From Different Regions in Relation to Biological Activity of Bacillus Thuringiensis Δ-endotoxin Cry1Ac. Arch Insect Biochem Physiol. 2014;87(4):201-13. PubMed PMID: 25195523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity in gut microflora of Helicoverpa armigera populations from different regions in relation to biological activity of Bacillus thuringiensis δ-endotoxin Cry1Ac. AU - Paramasiva,Inakarla, AU - Shouche,Yogesh, AU - Kulkarni,Girish Jayant, AU - Krishnayya,Pulipaka Venkata, AU - Akbar,Shaik Mohammed, AU - Sharma,Hari Chand, Y1 - 2014/09/04/ PY - 2014/9/9/entrez PY - 2014/9/10/pubmed PY - 2015/7/23/medline KW - 16S rRNA KW - Bacillus thuringiensis KW - Cry1Ac KW - Helicoverpa armigera; midgut microflora KW - transgenic crops SP - 201 EP - 13 JF - Archives of insect biochemistry and physiology JO - Arch Insect Biochem Physiol VL - 87 IS - 4 N2 - Transgenic crops expressing toxin proteins from Bacillus thuringiensis (Bt) have been deployed on a large scale for management of Helicoverpa armigera. Resistance to Bt toxins has been documented in several papers, and therefore, we examined the role of midgut microflora of H. armigera in its susceptibility to Bt toxins. The susceptibility of H. armigera to Bt toxin Cry1Ac was assessed using Log-dose-Probit analysis, and the microbial communities were identified by 16S rRNA sequencing. The H. armigera populations from nine locations harbored diverse microbial communities, and had some unique bacteria, suggesting a wide geographical variation in microbial community in the midgut of the pod borer larvae. Phylotypes belonging to 32 genera were identified in the H. armigera midgut in field populations from nine locations. Bacteria belonging to Enterobacteriaceae (Order Bacillales) were present in all the populations, and these may be the common members of the H. armigera larval midgut microflora. Presence and/or absence of certain species were linked to H. armigera susceptibility to Bt toxins, but there were no clear trends across locations. Variation in susceptibility of F1 neonates of H. armigera from different locations to the Bt toxin Cry1Ac was found to be 3.4-fold. These findings support the idea that insect migut microflora may influence the biological activity of Bt toxins. SN - 1520-6327 UR - https://www.unboundmedicine.com/medline/citation/25195523/Diversity_in_gut_microflora_of_Helicoverpa_armigera_populations_from_different_regions_in_relation_to_biological_activity_of_Bacillus_thuringiensis_δ_endotoxin_Cry1Ac_ DB - PRIME DP - Unbound Medicine ER -