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Extinction, applied after retrieval of auditory fear memory, selectively increases zinc-finger protein 268 and phosphorylated ribosomal protein S6 expression in prefrontal cortex and lateral amygdala.
Neurobiol Learn Mem. 2014 Nov; 115:78-85.NL

Abstract

Retrieval of consolidated memories induces a labile phase during which memory can be disrupted or updated through a reconsolidation process. A central component of behavioral updating during reconsolidation using a retrieval-extinction manipulation (Ret+Ext) is the synaptic removal of a calcium-permeable-α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptor (CP-AMPARs) in the lateral amygdala-a metabotropic GluR1 receptor (mGluR1) dependent mechanism. In the present study, we investigate the effect of Ret+Ext on the expression of molecular markers that could play a role in the reconsolidation process. Specifically, we tested the effects of Ret+Ext on the global expression of zinc-finger 268 protein (Zif268), a marker previously found to be implicated in memory reconsolidation, to confirm its occurrence after retrieval (Ret) and Ret+Ext. We also evaluated the global expression of phosphorylated ribosomal protein S6 (rpS6P), here proposed as a marker of the mGluR1-mediated memory process induced by Ret+Ext. The expression of both markers (zif268, rpS6P) was assessed by immunolocalization in prelimbic cortex (PRL), infralimbic cortex (IL), ventral subdivision of the lateral amygdala (LA) and hippocampus CA1 (CA1) in fear-conditioned rats. Our results showed that retrieval and Ret+Ext, but not extinction alone, increased Zif268 expression in prefrontal cortex and lateral amygdala. Ret+Ext, but not retrieval, retrieval followed by context exposure or extinction alone, increased the expression of rpS6P in prefrontal cortex and LA. In summary, (i) Zif268 increased after retrieval confirming that reconsolidation is engaged in our conditions, (ii) Zif268 increased after Ret+Ext confirming that it does not simply reflect an extinction or reconsolidation disruption (Zif268 level of expression should be lower in both cases) and (iii) rpS6P increased after Ret+Ext, but not after extinction, suggesting, as expected, a potential mGluR1 mediated molecular mechanism specific for Ret+Ext. Together with the Zif268 increase, our results suggest that the Ret+Ext induced memory process is more similar to reconsolidation updating than extinction facilitation.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Tedesco V
Department of Public Health & Community Medicine, University of Verona, 37134 Verona, Italy.
Roquet RF
Department of Psychology, University of Texas at Austin, 78712-0187 Austin, TX, USA.
DeMis J
Department of Psychology, University of Texas at Austin, 78712-0187 Austin, TX, USA.
Chiamulera C
Department of Public Health & Community Medicine, University of Verona, 37134 Verona, Italy.
Monfils MH
Department of Psychology, University of Texas at Austin, 78712-0187 Austin, TX, USA. Electronic address: marie.monfils@utexas.edu.

MeSH

Acoustic StimulationAnimalsBasolateral Nuclear ComplexConditioning, ClassicalEarly Growth Response Protein 1Extinction, PsychologicalFearMaleMental RecallPhosphorylationPrefrontal CortexRats, Sprague-DawleyRibosomal Protein S6

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25196703

Citation

Tedesco, Vincenzo, et al. "Extinction, Applied After Retrieval of Auditory Fear Memory, Selectively Increases Zinc-finger Protein 268 and Phosphorylated Ribosomal Protein S6 Expression in Prefrontal Cortex and Lateral Amygdala." Neurobiology of Learning and Memory, vol. 115, 2014, pp. 78-85.
Tedesco V, Roquet RF, DeMis J, et al. Extinction, applied after retrieval of auditory fear memory, selectively increases zinc-finger protein 268 and phosphorylated ribosomal protein S6 expression in prefrontal cortex and lateral amygdala. Neurobiol Learn Mem. 2014;115:78-85.
Tedesco, V., Roquet, R. F., DeMis, J., Chiamulera, C., & Monfils, M. H. (2014). Extinction, applied after retrieval of auditory fear memory, selectively increases zinc-finger protein 268 and phosphorylated ribosomal protein S6 expression in prefrontal cortex and lateral amygdala. Neurobiology of Learning and Memory, 115, 78-85. https://doi.org/10.1016/j.nlm.2014.08.015
Tedesco V, et al. Extinction, Applied After Retrieval of Auditory Fear Memory, Selectively Increases Zinc-finger Protein 268 and Phosphorylated Ribosomal Protein S6 Expression in Prefrontal Cortex and Lateral Amygdala. Neurobiol Learn Mem. 2014;115:78-85. PubMed PMID: 25196703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extinction, applied after retrieval of auditory fear memory, selectively increases zinc-finger protein 268 and phosphorylated ribosomal protein S6 expression in prefrontal cortex and lateral amygdala. AU - Tedesco,Vincenzo, AU - Roquet,Rheall F, AU - DeMis,John, AU - Chiamulera,Cristiano, AU - Monfils,Marie-H, Y1 - 2014/09/06/ PY - 2014/06/10/received PY - 2014/08/13/revised PY - 2014/08/29/accepted PY - 2014/9/9/entrez PY - 2014/9/10/pubmed PY - 2015/8/5/medline KW - Amygdala KW - Extinction KW - Fear conditioning KW - Prefrontal cortex KW - Reconsolidation KW - Retrieval KW - Zif268 KW - rpS6P SP - 78 EP - 85 JF - Neurobiology of learning and memory JO - Neurobiol Learn Mem VL - 115 N2 - Retrieval of consolidated memories induces a labile phase during which memory can be disrupted or updated through a reconsolidation process. A central component of behavioral updating during reconsolidation using a retrieval-extinction manipulation (Ret+Ext) is the synaptic removal of a calcium-permeable-α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptor (CP-AMPARs) in the lateral amygdala-a metabotropic GluR1 receptor (mGluR1) dependent mechanism. In the present study, we investigate the effect of Ret+Ext on the expression of molecular markers that could play a role in the reconsolidation process. Specifically, we tested the effects of Ret+Ext on the global expression of zinc-finger 268 protein (Zif268), a marker previously found to be implicated in memory reconsolidation, to confirm its occurrence after retrieval (Ret) and Ret+Ext. We also evaluated the global expression of phosphorylated ribosomal protein S6 (rpS6P), here proposed as a marker of the mGluR1-mediated memory process induced by Ret+Ext. The expression of both markers (zif268, rpS6P) was assessed by immunolocalization in prelimbic cortex (PRL), infralimbic cortex (IL), ventral subdivision of the lateral amygdala (LA) and hippocampus CA1 (CA1) in fear-conditioned rats. Our results showed that retrieval and Ret+Ext, but not extinction alone, increased Zif268 expression in prefrontal cortex and lateral amygdala. Ret+Ext, but not retrieval, retrieval followed by context exposure or extinction alone, increased the expression of rpS6P in prefrontal cortex and LA. In summary, (i) Zif268 increased after retrieval confirming that reconsolidation is engaged in our conditions, (ii) Zif268 increased after Ret+Ext confirming that it does not simply reflect an extinction or reconsolidation disruption (Zif268 level of expression should be lower in both cases) and (iii) rpS6P increased after Ret+Ext, but not after extinction, suggesting, as expected, a potential mGluR1 mediated molecular mechanism specific for Ret+Ext. Together with the Zif268 increase, our results suggest that the Ret+Ext induced memory process is more similar to reconsolidation updating than extinction facilitation. SN - 1095-9564 UR - https://www.unboundmedicine.com/medline/citation/25196703/Extinction_applied_after_retrieval_of_auditory_fear_memory_selectively_increases_zinc_finger_protein_268_and_phosphorylated_ribosomal_protein_S6_expression_in_prefrontal_cortex_and_lateral_amygdala_ DB - PRIME DP - Unbound Medicine ER -