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Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
Anal Biochem. 2014 Dec 15; 467:62-74.AB

Abstract

We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs). Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min. We showed the differences in the composition of GAG classes and the sulfation patterns between porcine articular cartilage and yellow ligament. In addition to the internal disaccharides described above, some saccharides derived from the nonreducing terminal were detected simultaneously. The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs. This method would help to establish comprehensive "GAGomic" analysis of biological tissues.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Osago H
Department of Biochemistry, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan. Electronic address: biochem1@med.shimane-u.ac.jp.
Shibata T
Center for Integrated Research in Science, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan.
Hara N
Department of Biochemistry, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan.
Kuwata S
Department of Orthopaedic Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan.
Kono M
Department of Orthopaedic Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan.
Uchio Y
Department of Orthopaedic Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan.
Tsuchiya M
Department of Biochemistry, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, Japan.

MeSH

AnimalsCartilage, ArticularChondroitin SulfatesChromatography, LiquidDermatan SulfateGlycosaminoglycansHeparitin SulfateHyaluronic AcidKeratan SulfateSpectrometry, Mass, Electrospray IonizationSwineTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25197028

Citation

Osago, Harumi, et al. "Quantitative Analysis of Glycosaminoglycans, Chondroitin/dermatan Sulfate, Hyaluronic Acid, Heparan Sulfate, and Keratan Sulfate By Liquid Chromatography-electrospray Ionization-tandem Mass Spectrometry." Analytical Biochemistry, vol. 467, 2014, pp. 62-74.
Osago H, Shibata T, Hara N, et al. Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry. Anal Biochem. 2014;467:62-74.
Osago, H., Shibata, T., Hara, N., Kuwata, S., Kono, M., Uchio, Y., & Tsuchiya, M. (2014). Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry. Analytical Biochemistry, 467, 62-74. https://doi.org/10.1016/j.ab.2014.08.005
Osago H, et al. Quantitative Analysis of Glycosaminoglycans, Chondroitin/dermatan Sulfate, Hyaluronic Acid, Heparan Sulfate, and Keratan Sulfate By Liquid Chromatography-electrospray Ionization-tandem Mass Spectrometry. Anal Biochem. 2014 Dec 15;467:62-74. PubMed PMID: 25197028.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry. AU - Osago,Harumi, AU - Shibata,Tomoko, AU - Hara,Nobumasa, AU - Kuwata,Suguru, AU - Kono,Michihaya, AU - Uchio,Yuji, AU - Tsuchiya,Mikako, Y1 - 2014/09/06/ PY - 2014/04/15/received PY - 2014/07/29/revised PY - 2014/08/05/accepted PY - 2014/9/9/entrez PY - 2014/9/10/pubmed PY - 2015/7/15/medline KW - Electrospray ionization (ESI) KW - Glycosaminoglycan (GAG) KW - High-sulfated disaccharide KW - Selected reaction monitoring (SRM) KW - Tandem mass spectrometry (MS/MS) SP - 62 EP - 74 JF - Analytical biochemistry JO - Anal Biochem VL - 467 N2 - We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs). Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min. We showed the differences in the composition of GAG classes and the sulfation patterns between porcine articular cartilage and yellow ligament. In addition to the internal disaccharides described above, some saccharides derived from the nonreducing terminal were detected simultaneously. The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs. This method would help to establish comprehensive "GAGomic" analysis of biological tissues. SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/25197028/Quantitative_analysis_of_glycosaminoglycans_chondroitin/dermatan_sulfate_hyaluronic_acid_heparan_sulfate_and_keratan_sulfate_by_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -
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