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2-Arachidonoylglycerol impairs human cytotrophoblast cells syncytialization: influence of endocannabinoid signalling in placental development.
Mol Cell Endocrinol. 2015 Jan 05; 399:386-94.MC

Abstract

A balanced cytotrophoblast cell turnover is crucial for placental development and anomalies in this process associated with gestational diseases. The endocannabinoid system (ECS) has emerged as a new player in several biological processes. However, its influence during placental development is still unknown. We report here the expression of the endocannabinoid 2-arachidonoylglycerol (2-AG) main metabolic enzymes in human cytotrophoblasts and syncytiotrophoblast. We also showed that 2-AG induced a decrease in placental alkaline phosphatase activity, human chorionic gonadotropin secretion and Leptin mRNA levels. Moreover, 2-AG reduced glial cell missing 1 and syncytin-2 transcription and the number of nuclei in syncytium. These effects were mediated by cannabinoid receptors and may result from 2-AG inhibition of the cAMP/PKA signalling pathway. Our data suggest that 2-AG may interfere with the biochemical and morphological differentiation of human cytotrophoblasts, through a CB receptor-dependent mechanism, shedding light on a role for the ECS in placental development.

Authors+Show Affiliations

Departamento de Ciências Biológicas, Laboratório de Bioquímica, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal; Instituto de Biologia Celular e Molecular da Universidade do Porto (IBMC), Porto, Portugal.Departmento de Bioquímica U38FCT, Faculdade de Medicina, Universidade do Porto, Porto, Portugal; Center for Biotechnology and Fine Chemistry, School of Biotechnology, Portuguese Catholic University, Porto, Portugal.Departamento de Ciências Biológicas, Laboratório de Bioquímica, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal; Instituto de Biologia Celular e Molecular da Universidade do Porto (IBMC), Porto, Portugal.Departamento de Ciências Biológicas, Laboratório de Bioquímica, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal; Instituto de Biologia Celular e Molecular da Universidade do Porto (IBMC), Porto, Portugal.Departamento de Ciências Biológicas, Laboratório de Bioquímica, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal; Instituto de Biologia Celular e Molecular da Universidade do Porto (IBMC), Porto, Portugal. Electronic address: george@ff.up.pt.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25199616

Citation

Costa, M A., et al. "2-Arachidonoylglycerol Impairs Human Cytotrophoblast Cells Syncytialization: Influence of Endocannabinoid Signalling in Placental Development." Molecular and Cellular Endocrinology, vol. 399, 2015, pp. 386-94.
Costa MA, Keating E, Fonseca BM, et al. 2-Arachidonoylglycerol impairs human cytotrophoblast cells syncytialization: influence of endocannabinoid signalling in placental development. Mol Cell Endocrinol. 2015;399:386-94.
Costa, M. A., Keating, E., Fonseca, B. M., Teixeira, N. A., & Correia-da-Silva, G. (2015). 2-Arachidonoylglycerol impairs human cytotrophoblast cells syncytialization: influence of endocannabinoid signalling in placental development. Molecular and Cellular Endocrinology, 399, 386-94. https://doi.org/10.1016/j.mce.2014.09.005
Costa MA, et al. 2-Arachidonoylglycerol Impairs Human Cytotrophoblast Cells Syncytialization: Influence of Endocannabinoid Signalling in Placental Development. Mol Cell Endocrinol. 2015 Jan 5;399:386-94. PubMed PMID: 25199616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 2-Arachidonoylglycerol impairs human cytotrophoblast cells syncytialization: influence of endocannabinoid signalling in placental development. AU - Costa,M A, AU - Keating,E, AU - Fonseca,B M, AU - Teixeira,N A, AU - Correia-da-Silva,G, Y1 - 2014/09/06/ PY - 2014/05/08/received PY - 2014/09/04/revised PY - 2014/09/04/accepted PY - 2014/9/10/entrez PY - 2014/9/10/pubmed PY - 2015/8/5/medline KW - 2-arachinoylglycerol KW - Endocannabinoid signalling KW - Human cytotrophoblasts KW - Placenta KW - Syncytialization SP - 386 EP - 94 JF - Molecular and cellular endocrinology JO - Mol Cell Endocrinol VL - 399 N2 - A balanced cytotrophoblast cell turnover is crucial for placental development and anomalies in this process associated with gestational diseases. The endocannabinoid system (ECS) has emerged as a new player in several biological processes. However, its influence during placental development is still unknown. We report here the expression of the endocannabinoid 2-arachidonoylglycerol (2-AG) main metabolic enzymes in human cytotrophoblasts and syncytiotrophoblast. We also showed that 2-AG induced a decrease in placental alkaline phosphatase activity, human chorionic gonadotropin secretion and Leptin mRNA levels. Moreover, 2-AG reduced glial cell missing 1 and syncytin-2 transcription and the number of nuclei in syncytium. These effects were mediated by cannabinoid receptors and may result from 2-AG inhibition of the cAMP/PKA signalling pathway. Our data suggest that 2-AG may interfere with the biochemical and morphological differentiation of human cytotrophoblasts, through a CB receptor-dependent mechanism, shedding light on a role for the ECS in placental development. SN - 1872-8057 UR - https://www.unboundmedicine.com/medline/citation/25199616/2_Arachidonoylglycerol_impairs_human_cytotrophoblast_cells_syncytialization:_influence_of_endocannabinoid_signalling_in_placental_development_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0303-7207(14)00276-7 DB - PRIME DP - Unbound Medicine ER -