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RNA interference of the P450 CYP6CM1 gene has different efficacy in B and Q biotypes of Bemisia tabaci.
Pest Manag Sci. 2015 Aug; 71(8):1175-81.PM

Abstract

BACKGROUND

Cytochrome P450 monooxygenases have been proven to be associated with high resistance in Bemisia tabaci B biotype (Middle East-Asia Minor 1 genetic group) and Q biotype (Mediterranean genetic group) to the neonicotinoid class of insecticides. In this study, the RNA interference (RNAi) effects on P450 CYP6CM1 gene expression, mortality and pesticide-detoxifying ability between B. tabaci B and Q biotypes were compared in an attempt to provide a basis for potential RNAi application in management of this pest.

RESULTS

Double-stranded RNAs (dsRNAs) of P450 CYP6CM1 genes corresponding to the B and Q biotypes were synthesised using specific primers and introduced into the insect body of B. tabaci adults through membrane feeding. The results showed that dsRNAs significantly silenced the target genes in B. tabaci with dsRNA concentration or treatment time, and silencing was more effective in B biotype than in Q biotype. Feeding dsRNAs led to high mortality in both biotypes, with higher mortality in B biotype (up to 85.88%) than in Q biotype (up to 56.40%). In addition, ability to detoxify imidacloprid and nicotine was inhibited in dsRNA-treated adults of both biotypes, more efficiently in B biotype than in Q biotype.

CONCLUSION

RNA interference of the P450 CYP6CM1 gene reduced gene expression, increased mortality, and inhibited the ability to detoxify a pesticide or a plant secondary metabolite in both biotypes of B. tabaci, with better efficacy in B biotype than in Q biotype.

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Publisher Full Text (DOI)

Authors+Show Affiliations

College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.

College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.

College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.

College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.

College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.

College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.

College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, MO, 65211, USA.

College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, 450002, China.

MeSH

AnimalsCytochrome P-450 Enzyme SystemFemaleGene ExpressionGossypiumHemipteraImidazolesInsect ProteinsInsecticide ResistanceInsecticidesMaleNeonicotinoidsNicotineNitro CompoundsRNA InterferenceRNA, Double-Stranded

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25200527

Citation

Li, Jingjing, et al. "RNA Interference of the P450 CYP6CM1 Gene Has Different Efficacy in B and Q Biotypes of Bemisia Tabaci." Pest Management Science, vol. 71, no. 8, 2015, pp. 1175-81.

Li J, Li X, Bai R, et al. RNA interference of the P450 CYP6CM1 gene has different efficacy in B and Q biotypes of Bemisia tabaci. Pest Manag Sci. 2015;71(8):1175-81.

Li, J., Li, X., Bai, R., Shi, Y., Tang, Q., An, S., Song, Q., & Yan, F. (2015). RNA interference of the P450 CYP6CM1 gene has different efficacy in B and Q biotypes of Bemisia tabaci. Pest Management Science, 71(8), 1175-81. https://doi.org/10.1002/ps.3903

Li J, et al. RNA Interference of the P450 CYP6CM1 Gene Has Different Efficacy in B and Q Biotypes of Bemisia Tabaci. Pest Manag Sci. 2015;71(8):1175-81. PubMed PMID: 25200527.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - RNA interference of the P450 CYP6CM1 gene has different efficacy in B and Q biotypes of Bemisia tabaci. AU - Li,Jingjing, AU - Li,Xiaomin, AU - Bai,Rune, AU - Shi,Yan, AU - Tang,Qingbo, AU - An,Shiheng, AU - Song,Qisheng, AU - Yan,Fengming, Y1 - 2014/10/08/ PY - 2014/02/21/received PY - 2014/07/23/revised PY - 2014/09/02/accepted PY - 2014/9/10/entrez PY - 2014/9/10/pubmed PY - 2016/4/7/medline KW - B biotype KW - Bemisia tabaci KW - CYP6CM1 gene, RNAi KW - Q biotype KW - cytochrome P450 SP - 1175 EP - 81 JF - Pest management science JO - Pest Manag Sci VL - 71 IS - 8 N2 - BACKGROUND: Cytochrome P450 monooxygenases have been proven to be associated with high resistance in Bemisia tabaci B biotype (Middle East-Asia Minor 1 genetic group) and Q biotype (Mediterranean genetic group) to the neonicotinoid class of insecticides. In this study, the RNA interference (RNAi) effects on P450 CYP6CM1 gene expression, mortality and pesticide-detoxifying ability between B. tabaci B and Q biotypes were compared in an attempt to provide a basis for potential RNAi application in management of this pest. RESULTS: Double-stranded RNAs (dsRNAs) of P450 CYP6CM1 genes corresponding to the B and Q biotypes were synthesised using specific primers and introduced into the insect body of B. tabaci adults through membrane feeding. The results showed that dsRNAs significantly silenced the target genes in B. tabaci with dsRNA concentration or treatment time, and silencing was more effective in B biotype than in Q biotype. Feeding dsRNAs led to high mortality in both biotypes, with higher mortality in B biotype (up to 85.88%) than in Q biotype (up to 56.40%). In addition, ability to detoxify imidacloprid and nicotine was inhibited in dsRNA-treated adults of both biotypes, more efficiently in B biotype than in Q biotype. CONCLUSION: RNA interference of the P450 CYP6CM1 gene reduced gene expression, increased mortality, and inhibited the ability to detoxify a pesticide or a plant secondary metabolite in both biotypes of B. tabaci, with better efficacy in B biotype than in Q biotype. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/25200527/RNA_interference_of_the_P450_CYP6CM1_gene_has_different_efficacy_in_B_and_Q_biotypes_of_Bemisia_tabaci_ DB - PRIME DP - Unbound Medicine ER -