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Characterization of microsatellites for the endangered Ruta oreojasme (Rutaceae) and cross-amplification in related species.

Abstract

PREMISE OF THE STUDY

Ruta oreojasme is an endangered species endemic to Gran Canaria (Canary Islands, Spain), where it occurs in small populations with disjunct distribution. Nothing is known about the genetic structure of these populations. •

METHODS AND RESULTS

Using a microsatellite-enriched library method, 10 microsatellite markers have been developed from R. oreojasme, all of which showed polymorphism. The transferability of the 10 markers was tested in two other Canarian endemic species, R. microcarpa and R. pinnata, as well as in the widespread species R. montana. •

CONCLUSIONS

Our results demonstrate the value of these newly developed microsatellite markers to investigate the genetic structure in R. oreojasme and show their potential applicability for population genetic studies in other Ruta species.

Links

Authors

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Source

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25202532

Citation

TY - JOUR T1 - Characterization of microsatellites for the endangered Ruta oreojasme (Rutaceae) and cross-amplification in related species. AU - Meloni,Marilena, AU - Reid,Andrea, AU - Conti,Elena, Y1 - 2013/03/22/ PY - 2013/4//ecollection PY - 2012/7/12/received PY - 2012/10/3/accepted PY - 2013/3/22/epublish PY - 2014/9/10/entrez PY - 2013/4/1/pubmed PY - 2013/4/1/medline KW - Ruta oreojasme KW - Rutaceae KW - genetic diversity KW - microsatellites SP - EP - JF - Applications in plant sciences JO - Appl Plant Sci VL - 1 IS - 4 N2 - UNLABELLED: • PREMISE OF THE STUDY: Ruta oreojasme is an endangered species endemic to Gran Canaria (Canary Islands, Spain), where it occurs in small populations with disjunct distribution. Nothing is known about the genetic structure of these populations. • METHODS AND RESULTS: Using a microsatellite-enriched library method, 10 microsatellite markers have been developed from R. oreojasme, all of which showed polymorphism. The transferability of the 10 markers was tested in two other Canarian endemic species, R. microcarpa and R. pinnata, as well as in the widespread species R. montana. • CONCLUSIONS: Our results demonstrate the value of these newly developed microsatellite markers to investigate the genetic structure in R. oreojasme and show their potential applicability for population genetic studies in other Ruta species. SN - 2168-0450 UR - https://www.unboundmedicine.com/medline/citation/25202532/Characterization_of_microsatellites_for_the_endangered_Ruta_oreojasme__Rutaceae__and_cross_amplification_in_related_species_ ER -