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[Hair roots induction and culture of Withania somnifera and its withanolide A synthesis].
Zhongguo Zhong Yao Za Zhi. 2014 Mar; 39(5):790-4.ZZ

Abstract

Withanolide A is a biologically active secondary metabolite occuring in roots and leaves of Withania somnifera. In the present study, adventitious roots from leaf explants of W. somnifera were induced for the production of withanolide-A by Agrobacterium tumefaciens strain C58C1 to obtain hair roots. Hair roots induction rate reached 30%. The withanolide A was determined by HPLC in different hair roots lines and different parts of W. somnifera. The average content of withanolide A in all hair roots lines were 1.96 times as high as that in wild-plant, the concentration of withanolide A in hair roots (1.783 mg x g(-1) dry weight) were 1.51 times as high as the roots of wild W. somnifera (1.180 mg x g(-1) dry weight), respectively. It is possible to obtain withanolide A from hair roots culture of W. somnifera.

Authors

Wang FY
No affiliation info available
Sun YM
No affiliation info available
Lv CP
No affiliation info available
Cheng MQ
No affiliation info available
Zhang L
No affiliation info available
Sun M
No affiliation info available

MeSH

Agrobacterium tumefaciensPlant ExtractsPlant RootsWithaniaWithanolides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

25204166

Citation

Wang, Feng-Ying, et al. "[Hair Roots Induction and Culture of Withania Somnifera and Its Withanolide a Synthesis]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 39, no. 5, 2014, pp. 790-4.
Wang FY, Sun YM, Lv CP, et al. [Hair roots induction and culture of Withania somnifera and its withanolide A synthesis]. Zhongguo Zhong Yao Za Zhi. 2014;39(5):790-4.
Wang, F. Y., Sun, Y. M., Lv, C. P., Cheng, M. Q., Zhang, L., & Sun, M. (2014). [Hair roots induction and culture of Withania somnifera and its withanolide A synthesis]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 39(5), 790-4.
Wang FY, et al. [Hair Roots Induction and Culture of Withania Somnifera and Its Withanolide a Synthesis]. Zhongguo Zhong Yao Za Zhi. 2014;39(5):790-4. PubMed PMID: 25204166.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Hair roots induction and culture of Withania somnifera and its withanolide A synthesis]. AU - Wang,Feng-Ying, AU - Sun,Yi-Ming, AU - Lv,Cui-Ping, AU - Cheng,Meng-Qi, AU - Zhang,Lai, AU - Sun,Min, PY - 2014/9/11/entrez PY - 2014/9/11/pubmed PY - 2014/10/1/medline SP - 790 EP - 4 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 39 IS - 5 N2 - Withanolide A is a biologically active secondary metabolite occuring in roots and leaves of Withania somnifera. In the present study, adventitious roots from leaf explants of W. somnifera were induced for the production of withanolide-A by Agrobacterium tumefaciens strain C58C1 to obtain hair roots. Hair roots induction rate reached 30%. The withanolide A was determined by HPLC in different hair roots lines and different parts of W. somnifera. The average content of withanolide A in all hair roots lines were 1.96 times as high as that in wild-plant, the concentration of withanolide A in hair roots (1.783 mg x g(-1) dry weight) were 1.51 times as high as the roots of wild W. somnifera (1.180 mg x g(-1) dry weight), respectively. It is possible to obtain withanolide A from hair roots culture of W. somnifera. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/25204166/[Hair_roots_induction_and_culture_of_Withania_somnifera_and_its_withanolide_A_synthesis]_ DB - PRIME DP - Unbound Medicine ER -

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