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The mechanism of astragaloside IV promoting sciatic nerve regeneration.
Neural Regen Res 2013; 8(24):2256-65NR

Abstract

3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol (astragaloside IV), the main active component of the traditional Chinese medicine astragalus membranaceus, has been shown to be neuroprotective. This study investigated whether astragaloside IV could promote the repair of injured sciatic nerve. Denervated sciatic nerve of mice was subjected to anastomosis. The mice were intraperitoneally injected with 10, 5, 2.5 mg/kg astragaloside IV per day for 8 consecutive days. Western blot assay and real-time PCR results demonstrated that growth-associated protein-43 expression was upregulated in mouse spinal cord segments L4-6 after intervention with 10, 5, 2.5 mg/kg astragaloside IV per day in a dose-dependent manner. Luxol fast blue staining and electrophysiological detection suggested that astragaloside IV elevated the number and diameter of myelinated nerve fibers, and simultaneously increased motor nerve conduction velocity and action potential amplitude in the sciatic nerve of mice. These results indicated that astragaloside IV contributed to sciatic nerve regeneration and functional recovery in mice. The mechanism underlying this effect may be associated with the upregulation of growth-associated protein-43 expression.

Authors+Show Affiliations

School of Pharmacutical Sciences, Jilin University, Changchun 130021, Jilin Province, China.Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25206535

Citation

Zhang, Xiaohong, and Jiajun Chen. "The Mechanism of Astragaloside IV Promoting Sciatic Nerve Regeneration." Neural Regeneration Research, vol. 8, no. 24, 2013, pp. 2256-65.
Zhang X, Chen J. The mechanism of astragaloside IV promoting sciatic nerve regeneration. Neural Regen Res. 2013;8(24):2256-65.
Zhang, X., & Chen, J. (2013). The mechanism of astragaloside IV promoting sciatic nerve regeneration. Neural Regeneration Research, 8(24), pp. 2256-65. doi:10.3969/j.issn.1673-5374.2013.24.005.
Zhang X, Chen J. The Mechanism of Astragaloside IV Promoting Sciatic Nerve Regeneration. Neural Regen Res. 2013 Aug 25;8(24):2256-65. PubMed PMID: 25206535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The mechanism of astragaloside IV promoting sciatic nerve regeneration. AU - Zhang,Xiaohong, AU - Chen,Jiajun, PY - 2013/04/05/received PY - 2013/07/26/accepted PY - 2014/9/11/entrez PY - 2014/9/11/pubmed PY - 2014/9/11/medline KW - astragaloside IV KW - axons KW - growth-associated protein-43 KW - myelinated nerve KW - nerve myelin sheath KW - neural regeneration KW - neuroregeneration KW - peripheral nerve injury KW - sciatic nerve KW - traditional Chinese medicine SP - 2256 EP - 65 JF - Neural regeneration research JO - Neural Regen Res VL - 8 IS - 24 N2 - 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol (astragaloside IV), the main active component of the traditional Chinese medicine astragalus membranaceus, has been shown to be neuroprotective. This study investigated whether astragaloside IV could promote the repair of injured sciatic nerve. Denervated sciatic nerve of mice was subjected to anastomosis. The mice were intraperitoneally injected with 10, 5, 2.5 mg/kg astragaloside IV per day for 8 consecutive days. Western blot assay and real-time PCR results demonstrated that growth-associated protein-43 expression was upregulated in mouse spinal cord segments L4-6 after intervention with 10, 5, 2.5 mg/kg astragaloside IV per day in a dose-dependent manner. Luxol fast blue staining and electrophysiological detection suggested that astragaloside IV elevated the number and diameter of myelinated nerve fibers, and simultaneously increased motor nerve conduction velocity and action potential amplitude in the sciatic nerve of mice. These results indicated that astragaloside IV contributed to sciatic nerve regeneration and functional recovery in mice. The mechanism underlying this effect may be associated with the upregulation of growth-associated protein-43 expression. SN - 1673-5374 UR - https://www.unboundmedicine.com/medline/citation/25206535/The_mechanism_of_astragaloside_IV_promoting_sciatic_nerve_regeneration_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25206535/ DB - PRIME DP - Unbound Medicine ER -