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Human umbilical cord mesenchymal stem cells alleviate nasal mucosa radiation damage in a guinea pig model.
J Cell Biochem 2015; 116(2):331-8JC

Abstract

Nasal complications after radiotherapy severely affect the quality of life of nasopharyngeal carcinoma patients, and there is a compelling need to find novel therapies for nasal epithelial cell radiation damage. Therefore, we investigated the therapeutic effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) in guinea pig model of nasal mucosa radiation damage and explored its therapeutic mechanism. Cultured hUC-MSCs were injected intravenously immediately after radiation in the nasal mucosa-radiation-damage guinea pig model. Migration of hUC-MSCs into the nasal mucosa and the potential for differentiation into nasal epithelial cells were evaluated by immunofluorescence. The therapeutic effects of hUC-MSCs were evaluated by mucus clearance time (MCT), degree of nasal mucosa edema, and the nasal mucosa cilia form and coverage ratio. Results indicate that the hUC-MSCs migrated to the nasal mucosa lamina propria and did not differentiate into nasal epithelial cells in this model. The MCT and degree of mucosal edema were improved at 1 week and 1 month after radiation, respectively, but no difference was found at 3 months and 6 months after radiation. The nasal mucosa cilia form and coverage ratio was not improved 6 months after radiation. Thus, hUC-MSCs can migrate to the nasal mucosa lamina propria and improve MCT and mucosa edema within a short time period, but these cells are unable to differentiate into nasal epithelial cells and improve nasal epithelial regeneration in the nasal mucosa radiation damage guinea pig model.

Authors+Show Affiliations

Department of Otolaryngology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25209829

Citation

Duan, Hong-Gang, et al. "Human Umbilical Cord Mesenchymal Stem Cells Alleviate Nasal Mucosa Radiation Damage in a Guinea Pig Model." Journal of Cellular Biochemistry, vol. 116, no. 2, 2015, pp. 331-8.
Duan HG, Ji F, Zheng CQ, et al. Human umbilical cord mesenchymal stem cells alleviate nasal mucosa radiation damage in a guinea pig model. J Cell Biochem. 2015;116(2):331-8.
Duan, H. G., Ji, F., Zheng, C. Q., Wang, C. H., & Li, J. (2015). Human umbilical cord mesenchymal stem cells alleviate nasal mucosa radiation damage in a guinea pig model. Journal of Cellular Biochemistry, 116(2), pp. 331-8. doi:10.1002/jcb.24975.
Duan HG, et al. Human Umbilical Cord Mesenchymal Stem Cells Alleviate Nasal Mucosa Radiation Damage in a Guinea Pig Model. J Cell Biochem. 2015;116(2):331-8. PubMed PMID: 25209829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human umbilical cord mesenchymal stem cells alleviate nasal mucosa radiation damage in a guinea pig model. AU - Duan,Hong-Gang, AU - Ji,Fang, AU - Zheng,Chun-Quan, AU - Wang,Chun-Hua, AU - Li,Jing, PY - 2014/06/15/received PY - 2014/09/05/accepted PY - 2014/9/12/entrez PY - 2014/9/12/pubmed PY - 2015/8/22/medline KW - MESENCHYMAL STEM CELLS KW - NASOPHARYNGEAL CARCINOMA KW - RADIATION DAMAGE KW - STEM CELL TRANSPLANTATION SP - 331 EP - 8 JF - Journal of cellular biochemistry JO - J. Cell. Biochem. VL - 116 IS - 2 N2 - Nasal complications after radiotherapy severely affect the quality of life of nasopharyngeal carcinoma patients, and there is a compelling need to find novel therapies for nasal epithelial cell radiation damage. Therefore, we investigated the therapeutic effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) in guinea pig model of nasal mucosa radiation damage and explored its therapeutic mechanism. Cultured hUC-MSCs were injected intravenously immediately after radiation in the nasal mucosa-radiation-damage guinea pig model. Migration of hUC-MSCs into the nasal mucosa and the potential for differentiation into nasal epithelial cells were evaluated by immunofluorescence. The therapeutic effects of hUC-MSCs were evaluated by mucus clearance time (MCT), degree of nasal mucosa edema, and the nasal mucosa cilia form and coverage ratio. Results indicate that the hUC-MSCs migrated to the nasal mucosa lamina propria and did not differentiate into nasal epithelial cells in this model. The MCT and degree of mucosal edema were improved at 1 week and 1 month after radiation, respectively, but no difference was found at 3 months and 6 months after radiation. The nasal mucosa cilia form and coverage ratio was not improved 6 months after radiation. Thus, hUC-MSCs can migrate to the nasal mucosa lamina propria and improve MCT and mucosa edema within a short time period, but these cells are unable to differentiate into nasal epithelial cells and improve nasal epithelial regeneration in the nasal mucosa radiation damage guinea pig model. SN - 1097-4644 UR - https://www.unboundmedicine.com/medline/citation/25209829/Human_umbilical_cord_mesenchymal_stem_cells_alleviate_nasal_mucosa_radiation_damage_in_a_guinea_pig_model_ L2 - https://doi.org/10.1002/jcb.24975 DB - PRIME DP - Unbound Medicine ER -