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Purification and partial biochemical characterization of polyphenol oxidase from mango (Mangifera indica cv. Manila).
J Agric Food Chem 2014; 62(40):9832-40JA

Abstract

Polyphenol oxidase (PPO) is an enzyme widely distributed in the plant kingdom that has been detected in most fruits and vegetables. PPO was extracted and purified from Manila mango (Mangifera indica), and its biochemical properties were studied. PPO was purified 216-fold by hydrophobic interaction and ion exchange chromatography. PPO was purified to homogeneity, and the estimated PPO molecular weight (MW) by SDS-PAGE was ≈31.5 kDa. However, a MW of 65 kDa was determined by gel filtration, indicating a dimeric structure for the native PPO. The isolated PPO showed the highest affinity to pyrogallol (Km = 2.77 mM) followed by 4-methylcatechol (Km = 3.14 mM) and catechol (Km = 15.14 mM). The optimum pH for activity was 6.0. PPO was stable in the temperature range of 20-70 °C. PPO activity was completely inhibited by tropolone, ascorbic acid, sodium metabisulfite, and kojic acid at 0.1 mM.

Authors+Show Affiliations

Unidad Profesional Interdisciplinaria de Biotecnologı́a, Departamento de Bioingenierı́a, Instituto Politécnico Nacional , Acueducto s/n, Col. La Laguna Ticoman, Delegación Gustavo A. Madero, C.P. 07340, México, D.F., Mexico.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25211397

Citation

Palma-Orozco, Gisela, et al. "Purification and Partial Biochemical Characterization of Polyphenol Oxidase From Mango (Mangifera Indica Cv. Manila)." Journal of Agricultural and Food Chemistry, vol. 62, no. 40, 2014, pp. 9832-40.
Palma-Orozco G, Marrufo-Hernández NA, Sampedro JG, et al. Purification and partial biochemical characterization of polyphenol oxidase from mango (Mangifera indica cv. Manila). J Agric Food Chem. 2014;62(40):9832-40.
Palma-Orozco, G., Marrufo-Hernández, N. A., Sampedro, J. G., & Nájera, H. (2014). Purification and partial biochemical characterization of polyphenol oxidase from mango (Mangifera indica cv. Manila). Journal of Agricultural and Food Chemistry, 62(40), pp. 9832-40. doi:10.1021/jf5029784.
Palma-Orozco G, et al. Purification and Partial Biochemical Characterization of Polyphenol Oxidase From Mango (Mangifera Indica Cv. Manila). J Agric Food Chem. 2014 Oct 8;62(40):9832-40. PubMed PMID: 25211397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification and partial biochemical characterization of polyphenol oxidase from mango (Mangifera indica cv. Manila). AU - Palma-Orozco,Gisela, AU - Marrufo-Hernández,Norma A, AU - Sampedro,José G, AU - Nájera,Hugo, Y1 - 2014/09/26/ PY - 2014/9/12/entrez PY - 2014/9/12/pubmed PY - 2015/5/23/medline KW - Manila mango (Mangifera indica) KW - enzymatic browning KW - inhibitors KW - polyphenol oxidase KW - purification SP - 9832 EP - 40 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 62 IS - 40 N2 - Polyphenol oxidase (PPO) is an enzyme widely distributed in the plant kingdom that has been detected in most fruits and vegetables. PPO was extracted and purified from Manila mango (Mangifera indica), and its biochemical properties were studied. PPO was purified 216-fold by hydrophobic interaction and ion exchange chromatography. PPO was purified to homogeneity, and the estimated PPO molecular weight (MW) by SDS-PAGE was ≈31.5 kDa. However, a MW of 65 kDa was determined by gel filtration, indicating a dimeric structure for the native PPO. The isolated PPO showed the highest affinity to pyrogallol (Km = 2.77 mM) followed by 4-methylcatechol (Km = 3.14 mM) and catechol (Km = 15.14 mM). The optimum pH for activity was 6.0. PPO was stable in the temperature range of 20-70 °C. PPO activity was completely inhibited by tropolone, ascorbic acid, sodium metabisulfite, and kojic acid at 0.1 mM. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/25211397/Purification_and_partial_biochemical_characterization_of_polyphenol_oxidase_from_mango__Mangifera_indica_cv__Manila__ L2 - https://dx.doi.org/10.1021/jf5029784 DB - PRIME DP - Unbound Medicine ER -