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Functional expression of chloride channels and their roles in the cell cycle and cell proliferation in highly differentiated nasopharyngeal carcinoma cells.
Physiol Rep 2014; 2(9)PR

Abstract

We previously demonstrated that the growth of the poorly differentiated nasopharyngeal carcinoma cells (CNE-2Z) was more dependent on the activities of volume-activated chloride channels than that of the normal nasopharyngeal epithelial cells (NP69-SV40T). However, the activities and roles of such volume-activated chloride channels in highly differentiated nasopharyngeal carcinoma cells (CNE-1) are not clarified. In this study, it was found that a volume-activated chloride current and a regulatory volume decrease (RVD) were induced by 47% hypotonic challenges. The current density and the capacity of RVD in the highly differentiated CNE-1 cells were lower than those in the poorly differentiated CNE-2Z cells, and higher than those in the normal cells (NP69-SV40T). The chloride channel blockers, 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and tamoxifen inhibited the current and RVD. Depletion of intracellular Cl(-) abolished the RVD. The chloride channel blockers reversibly inhibited cell proliferation in a concentration- and time-dependent manner, and arrested cells at the G0/G1 phases, but did not change cell viability. The sensitivity of the three cell lines to the chloride channel blockers was different, with the highest in poorly differentiated cells (CNE-2Z) and the lowest in the normal cells (NP69-SV40T). ClC-3 proteins were expressed in the three cells and distributed inside the cells as well as on the cell membrane. In conclusion, the highly differentiated nasopharyngeal carcinoma CNE-1 cells functionally expressed the volume-activated chloride channels, which may play important roles in controlling cell proliferation through modulating the cell cycle, and may be associated with cell differentiation. Chloride channels may be a potential target of anticancer therapy.

Authors+Show Affiliations

Department of Physiology, Medical College, Jinan University, Guangzhou, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.Department of Physiology, Medical College, Jinan University, Guangzhou, China.Department of Physiology, Medical College, Jinan University, Guangzhou, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.Department of Physiology, Medical College, Jinan University, Guangzhou, China.Department of Physiology, Medical College, Jinan University, Guangzhou, China.Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.Department of Physiology, Medical College, Jinan University, Guangzhou, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25214521

Citation

Huang, Weiyuan, et al. "Functional Expression of Chloride Channels and Their Roles in the Cell Cycle and Cell Proliferation in Highly Differentiated Nasopharyngeal Carcinoma Cells." Physiological Reports, vol. 2, no. 9, 2014.
Huang W, Liu M, Zhu L, et al. Functional expression of chloride channels and their roles in the cell cycle and cell proliferation in highly differentiated nasopharyngeal carcinoma cells. Physiol Rep. 2014;2(9).
Huang, W., Liu, M., Zhu, L., Liu, S., Luo, H., Ma, L., ... Wang, L. (2014). Functional expression of chloride channels and their roles in the cell cycle and cell proliferation in highly differentiated nasopharyngeal carcinoma cells. Physiological Reports, 2(9), doi:10.14814/phy2.12137.
Huang W, et al. Functional Expression of Chloride Channels and Their Roles in the Cell Cycle and Cell Proliferation in Highly Differentiated Nasopharyngeal Carcinoma Cells. Physiol Rep. 2014 Sep 1;2(9) PubMed PMID: 25214521.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional expression of chloride channels and their roles in the cell cycle and cell proliferation in highly differentiated nasopharyngeal carcinoma cells. AU - Huang,Weiyuan, AU - Liu,Mei, AU - Zhu,Linyan, AU - Liu,Shanwen, AU - Luo,Hai, AU - Ma,Lianshun, AU - Wang,Haibo, AU - Lu,Ruiling, AU - Sun,Xiaoxue, AU - Chen,Lixin, AU - Wang,Liwei, Y1 - 2014/09/11/ PY - 2014/9/13/entrez PY - 2014/9/13/pubmed PY - 2014/9/13/medline KW - Cell cycle KW - cell proliferation KW - chloride channels KW - nasopharyngeal carcinoma KW - regulatory volume decrease JF - Physiological reports JO - Physiol Rep VL - 2 IS - 9 N2 - We previously demonstrated that the growth of the poorly differentiated nasopharyngeal carcinoma cells (CNE-2Z) was more dependent on the activities of volume-activated chloride channels than that of the normal nasopharyngeal epithelial cells (NP69-SV40T). However, the activities and roles of such volume-activated chloride channels in highly differentiated nasopharyngeal carcinoma cells (CNE-1) are not clarified. In this study, it was found that a volume-activated chloride current and a regulatory volume decrease (RVD) were induced by 47% hypotonic challenges. The current density and the capacity of RVD in the highly differentiated CNE-1 cells were lower than those in the poorly differentiated CNE-2Z cells, and higher than those in the normal cells (NP69-SV40T). The chloride channel blockers, 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and tamoxifen inhibited the current and RVD. Depletion of intracellular Cl(-) abolished the RVD. The chloride channel blockers reversibly inhibited cell proliferation in a concentration- and time-dependent manner, and arrested cells at the G0/G1 phases, but did not change cell viability. The sensitivity of the three cell lines to the chloride channel blockers was different, with the highest in poorly differentiated cells (CNE-2Z) and the lowest in the normal cells (NP69-SV40T). ClC-3 proteins were expressed in the three cells and distributed inside the cells as well as on the cell membrane. In conclusion, the highly differentiated nasopharyngeal carcinoma CNE-1 cells functionally expressed the volume-activated chloride channels, which may play important roles in controlling cell proliferation through modulating the cell cycle, and may be associated with cell differentiation. Chloride channels may be a potential target of anticancer therapy. SN - 2051-817X UR - https://www.unboundmedicine.com/medline/citation/25214521/Functional_expression_of_chloride_channels_and_their_roles_in_the_cell_cycle_and_cell_proliferation_in_highly_differentiated_nasopharyngeal_carcinoma_cells_ L2 - https://doi.org/10.14814/phy2.12137 DB - PRIME DP - Unbound Medicine ER -