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Long-term intake of a high prebiotic fiber diet but not high protein reduces metabolic risk after a high fat challenge and uniquely alters gut microbiota and hepatic gene expression.
Nutr Res 2014; 34(9):789-96NR

Abstract

A mismatch between early developmental diet and adulthood may increase obesity risk. Our objective was to determine the effects of re-matching rats to their weaning diets high in protein or fiber after transient high-fat/high-sucrose challenge in adulthood. We hypothesize that a long-term high fiber diet will be associated with a gut microbiota and hepatic gene expression reflective of reduced adiposity. Wistar rat pups were fed a control (C), high prebiotic fiber (HF), or high protein (HP) diet from 3-15 weeks of age; a high-fat/high-sucrose diet from 15-21 weeks; their respective C, HF, or HP diets from 21-25 weeks. Gut microbiota of cecal contents and hepatic gene expression were measured when rats were terminated at 25 weeks of age. HF rats had higher total bacteria, bifidobacteria and Bacteroides/Prevotella spp than C and HP at 25 weeks (P < 0.05). Firmicutes, especially Clostridium leptum, decreased in HF compared to C and HP (P < .05). The ratio of Firmicutes:Bacteroidetes was markedly lower in HF versus C and HP at 25 weeks (P < .05). HF decreased hepatic cholesterol content compared to HP and C at 25 weeks. HF and HP increased 3-hydroxy-3-methylglutaryl-CoA reductase mRNA and decreased lecithin-cholesterol acyltransferase mRNA compared to C (P < .05). In conclusion, re-matching rats to a HF but not HP diet attenuated the typical increase in Firmicutes:Bacteroidetes ratio associated with consumption of a high fat diet. Lower hepatic cholesterol with long-term HF diet intake may be related to alterations in gut microbiota and hepatic lipid metabolism.

Authors+Show Affiliations

Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada T2N 1N4. Electronic address: dcsaha@ucalgary.ca.Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada T2N 1N4; Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB, Canada T2N 4N1. Electronic address: reimer@ucalgary.ca.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25217504

Citation

Saha, Dolan C., and Raylene A. Reimer. "Long-term Intake of a High Prebiotic Fiber Diet but Not High Protein Reduces Metabolic Risk After a High Fat Challenge and Uniquely Alters Gut Microbiota and Hepatic Gene Expression." Nutrition Research (New York, N.Y.), vol. 34, no. 9, 2014, pp. 789-96.
Saha DC, Reimer RA. Long-term intake of a high prebiotic fiber diet but not high protein reduces metabolic risk after a high fat challenge and uniquely alters gut microbiota and hepatic gene expression. Nutr Res. 2014;34(9):789-96.
Saha, D. C., & Reimer, R. A. (2014). Long-term intake of a high prebiotic fiber diet but not high protein reduces metabolic risk after a high fat challenge and uniquely alters gut microbiota and hepatic gene expression. Nutrition Research (New York, N.Y.), 34(9), pp. 789-96. doi:10.1016/j.nutres.2014.08.004.
Saha DC, Reimer RA. Long-term Intake of a High Prebiotic Fiber Diet but Not High Protein Reduces Metabolic Risk After a High Fat Challenge and Uniquely Alters Gut Microbiota and Hepatic Gene Expression. Nutr Res. 2014;34(9):789-96. PubMed PMID: 25217504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term intake of a high prebiotic fiber diet but not high protein reduces metabolic risk after a high fat challenge and uniquely alters gut microbiota and hepatic gene expression. AU - Saha,Dolan C, AU - Reimer,Raylene A, Y1 - 2014/08/23/ PY - 2014/05/12/received PY - 2014/08/13/revised PY - 2014/08/18/accepted PY - 2014/9/14/entrez PY - 2014/9/14/pubmed PY - 2015/5/26/medline KW - Cholesterol KW - Gut microbiota KW - Lipid metabolism KW - Obesity KW - Prebiotic fiber KW - Rat SP - 789 EP - 96 JF - Nutrition research (New York, N.Y.) JO - Nutr Res VL - 34 IS - 9 N2 - A mismatch between early developmental diet and adulthood may increase obesity risk. Our objective was to determine the effects of re-matching rats to their weaning diets high in protein or fiber after transient high-fat/high-sucrose challenge in adulthood. We hypothesize that a long-term high fiber diet will be associated with a gut microbiota and hepatic gene expression reflective of reduced adiposity. Wistar rat pups were fed a control (C), high prebiotic fiber (HF), or high protein (HP) diet from 3-15 weeks of age; a high-fat/high-sucrose diet from 15-21 weeks; their respective C, HF, or HP diets from 21-25 weeks. Gut microbiota of cecal contents and hepatic gene expression were measured when rats were terminated at 25 weeks of age. HF rats had higher total bacteria, bifidobacteria and Bacteroides/Prevotella spp than C and HP at 25 weeks (P < 0.05). Firmicutes, especially Clostridium leptum, decreased in HF compared to C and HP (P < .05). The ratio of Firmicutes:Bacteroidetes was markedly lower in HF versus C and HP at 25 weeks (P < .05). HF decreased hepatic cholesterol content compared to HP and C at 25 weeks. HF and HP increased 3-hydroxy-3-methylglutaryl-CoA reductase mRNA and decreased lecithin-cholesterol acyltransferase mRNA compared to C (P < .05). In conclusion, re-matching rats to a HF but not HP diet attenuated the typical increase in Firmicutes:Bacteroidetes ratio associated with consumption of a high fat diet. Lower hepatic cholesterol with long-term HF diet intake may be related to alterations in gut microbiota and hepatic lipid metabolism. SN - 1879-0739 UR - https://www.unboundmedicine.com/medline/citation/25217504/Long_term_intake_of_a_high_prebiotic_fiber_diet_but_not_high_protein_reduces_metabolic_risk_after_a_high_fat_challenge_and_uniquely_alters_gut_microbiota_and_hepatic_gene_expression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0271-5317(14)00142-0 DB - PRIME DP - Unbound Medicine ER -