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Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease.
Respir Res 2014; 15:114RR

Abstract

BACKGROUND

There has been increasing interest in the use of newer, culture-independent techniques to study the airway microbiome of COPD patients. We investigated the relationships between the three common potentially pathogenic microorganisms (PPMs) Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis, as detected by quantitative PCR (qPCR), and inflammation and health status in stable patients in the London COPD cohort.

METHODS

We prospectively collected sputum, serum and plasma samples for analysis of airway bacterial presence and load, and airway and systemic inflammation from 99 stable COPD patients between January 2011 and October 2012. Health status was measured with St George's Respiratory Questionnaire and COPD Assessment Test.

RESULTS

Airway inflammation and plasma fibrinogen, but not C-reactive protein, were greater in samples with PPM detection (p < 0.001, p = 0.049 and p = 0.261, respectively). Increasing total bacterial load was associated with increasing airway (p < 0.01) but not systemic inflammation (p > 0.05). Samples with high total bacterial loads had significantly higher airway inflammation than both samples without PPM detection and those with lower loads. Haemophilus influenzae presence was associated with significantly higher levels of airway but not systemic inflammation for all given pathogen loads (p < 0.05), and was significantly greater than with other PPMs. No association was observed between inflammation and health status (p > 0.05).

CONCLUSIONS

Airway and systemic inflammation, as measured by fibrinogen, is greater in stable COPD patients with PPMs detected using the culture-independent qPCR technique. The airway, but not systemic inflammatory response, appears to have a total pathogen-load threshold and appears attributable to Haemophilus influenzae. However, discordance between inflammation and health status was observed.

Authors+Show Affiliations

Airway Disease Section, National Heart and Lung Institute, Imperial College, Dovehouse Street, London SW3 6LR, UK. richa.singh@imperial.ac.uk.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25218165

Citation

Singh, Richa, et al. "Inflammatory Thresholds and the Species-specific Effects of Colonising Bacteria in Stable Chronic Obstructive Pulmonary Disease." Respiratory Research, vol. 15, 2014, p. 114.
Singh R, Mackay AJ, Patel AR, et al. Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease. Respir Res. 2014;15:114.
Singh, R., Mackay, A. J., Patel, A. R., Garcha, D. S., Kowlessar, B. S., Brill, S. E., ... Wedzicha, J. A. (2014). Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease. Respiratory Research, 15, p. 114. doi:10.1186/s12931-014-0114-1.
Singh R, et al. Inflammatory Thresholds and the Species-specific Effects of Colonising Bacteria in Stable Chronic Obstructive Pulmonary Disease. Respir Res. 2014 Sep 14;15:114. PubMed PMID: 25218165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease. AU - Singh,Richa, AU - Mackay,Alexander J, AU - Patel,Anant Rc, AU - Garcha,Davinder S, AU - Kowlessar,Beverly S, AU - Brill,Simon E, AU - Donnelly,Louise E, AU - Barnes,Peter J, AU - Donaldson,Gavin C, AU - Wedzicha,Jadwiga A, Y1 - 2014/09/14/ PY - 2014/06/27/received PY - 2014/09/02/accepted PY - 2014/9/15/entrez PY - 2014/9/15/pubmed PY - 2015/4/18/medline SP - 114 EP - 114 JF - Respiratory research JO - Respir. Res. VL - 15 N2 - BACKGROUND: There has been increasing interest in the use of newer, culture-independent techniques to study the airway microbiome of COPD patients. We investigated the relationships between the three common potentially pathogenic microorganisms (PPMs) Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis, as detected by quantitative PCR (qPCR), and inflammation and health status in stable patients in the London COPD cohort. METHODS: We prospectively collected sputum, serum and plasma samples for analysis of airway bacterial presence and load, and airway and systemic inflammation from 99 stable COPD patients between January 2011 and October 2012. Health status was measured with St George's Respiratory Questionnaire and COPD Assessment Test. RESULTS: Airway inflammation and plasma fibrinogen, but not C-reactive protein, were greater in samples with PPM detection (p < 0.001, p = 0.049 and p = 0.261, respectively). Increasing total bacterial load was associated with increasing airway (p < 0.01) but not systemic inflammation (p > 0.05). Samples with high total bacterial loads had significantly higher airway inflammation than both samples without PPM detection and those with lower loads. Haemophilus influenzae presence was associated with significantly higher levels of airway but not systemic inflammation for all given pathogen loads (p < 0.05), and was significantly greater than with other PPMs. No association was observed between inflammation and health status (p > 0.05). CONCLUSIONS: Airway and systemic inflammation, as measured by fibrinogen, is greater in stable COPD patients with PPMs detected using the culture-independent qPCR technique. The airway, but not systemic inflammatory response, appears to have a total pathogen-load threshold and appears attributable to Haemophilus influenzae. However, discordance between inflammation and health status was observed. SN - 1465-993X UR - https://www.unboundmedicine.com/medline/citation/25218165/Inflammatory_thresholds_and_the_species_specific_effects_of_colonising_bacteria_in_stable_chronic_obstructive_pulmonary_disease_ L2 - https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-014-0114-1 DB - PRIME DP - Unbound Medicine ER -