Tags

Type your tag names separated by a space and hit enter

Highly sensitive graphene-Pt nanocomposites amperometric biosensor and its application in living cell H2O2 detection.
Anal Chem. 2014 Oct 07; 86(19):9459-65.AC

Abstract

A sensitive hydrogen peroxide (H2O2) sensor was constructed based on graphene-Pt (RGO-Pt) nanocomposites and used to measure the release of H2O2 from living cells. The graphene and Pt nanoparticles (Pt NPs) were modified on glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of K2PtCl6 solution, respectively. Through characterization by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), it was observed that the electrodeposited Pt NPs were densely covered and well distributed on the entire graphene surface. Electrochemical study demonstrates that the RGO-Pt nanocomposites modified glassy carbon electrode exhibited a high peak current and low overpotential toward the reduction of H2O2. The relevant detection limit of H2O2 is ∼0.2 μM with a wide linear range from 0.5 μM to 3.475 mM, displaying a much higher sensitivity (459 ± 3 mA M(-1) cm(-2), n = 5) than that of Pt nanoparticles or graphene modified electrode. This novel biosensor can measure the H2O2 release from living cells because of its low detection limit, wide linear range, and higher sensitivity.

Authors+Show Affiliations

State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University , Nanjing 210096, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25222618

Citation

Zhang, Yuanyuan, et al. "Highly Sensitive graphene-Pt Nanocomposites Amperometric Biosensor and Its Application in Living Cell H2O2 Detection." Analytical Chemistry, vol. 86, no. 19, 2014, pp. 9459-65.
Zhang Y, Bai X, Wang X, et al. Highly sensitive graphene-Pt nanocomposites amperometric biosensor and its application in living cell H2O2 detection. Anal Chem. 2014;86(19):9459-65.
Zhang, Y., Bai, X., Wang, X., Shiu, K. K., Zhu, Y., & Jiang, H. (2014). Highly sensitive graphene-Pt nanocomposites amperometric biosensor and its application in living cell H2O2 detection. Analytical Chemistry, 86(19), 9459-65. https://doi.org/10.1021/ac5009699
Zhang Y, et al. Highly Sensitive graphene-Pt Nanocomposites Amperometric Biosensor and Its Application in Living Cell H2O2 Detection. Anal Chem. 2014 Oct 7;86(19):9459-65. PubMed PMID: 25222618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly sensitive graphene-Pt nanocomposites amperometric biosensor and its application in living cell H2O2 detection. AU - Zhang,Yuanyuan, AU - Bai,Xiaoyun, AU - Wang,Xuemei, AU - Shiu,Kwok-Keung, AU - Zhu,Yanliang, AU - Jiang,Hui, Y1 - 2014/09/15/ PY - 2014/9/16/entrez PY - 2014/9/16/pubmed PY - 2015/8/25/medline SP - 9459 EP - 65 JF - Analytical chemistry JO - Anal Chem VL - 86 IS - 19 N2 - A sensitive hydrogen peroxide (H2O2) sensor was constructed based on graphene-Pt (RGO-Pt) nanocomposites and used to measure the release of H2O2 from living cells. The graphene and Pt nanoparticles (Pt NPs) were modified on glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of K2PtCl6 solution, respectively. Through characterization by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), it was observed that the electrodeposited Pt NPs were densely covered and well distributed on the entire graphene surface. Electrochemical study demonstrates that the RGO-Pt nanocomposites modified glassy carbon electrode exhibited a high peak current and low overpotential toward the reduction of H2O2. The relevant detection limit of H2O2 is ∼0.2 μM with a wide linear range from 0.5 μM to 3.475 mM, displaying a much higher sensitivity (459 ± 3 mA M(-1) cm(-2), n = 5) than that of Pt nanoparticles or graphene modified electrode. This novel biosensor can measure the H2O2 release from living cells because of its low detection limit, wide linear range, and higher sensitivity. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/25222618/Highly_sensitive_graphene_Pt_nanocomposites_amperometric_biosensor_and_its_application_in_living_cell_H2O2_detection_ L2 - https://doi.org/10.1021/ac5009699 DB - PRIME DP - Unbound Medicine ER -