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Temperature influences histone methylation and mRNA expression of the Jmj-C histone-demethylase orthologues during the early development of the oyster Crassostrea gigas.
Mar Genomics. 2015 Feb; 19:23-30.MG

Abstract

In many groups, epigenetic mechanisms influence developmental gene regulation under environmental inputs. The Pacific oyster Crassostrea gigas belongs to lophotrochozoans and its larval development is highly dependent on temperature, but the role of epigenetic mechanisms in this context is unknown despite high levels of the recently characterized Jumonji histone demethylase (JHDM) orthologues (Cg_Jumonji) suggesting a physiological relevance of histone methylation in the oyster development. Because in other species alterations of the histone methylation pattern have deleterious outcomes, we investigated the influence of temperature during the oyster larval life on histone methylation and JHDM expression. To shed light on this point, oyster embryonic and early larval development experiments were carried out at different temperatures (18 °C, 25 °C and 32 °C). Histone methylation levels were investigated using fluorescent ELISA at 6 and 24h post-fertilization. When compared to the 25 °C group, at 18 °C H3K4, H3K9 and H3K27 residues were hypomethylated at 6h post fertilization (hpf) and hypermethylated at 24 hpf. In contrast, at 32 °C, 6hpf animals present a dramatic hypermethylation (ca. 4-fold) of all examined residues, which is minored but sustained at 24 hpf. RT-qPCR investigations of the mRNA expression of the nine oyster JHDMs, showed gene- and stage-specific temperature sensitivities throughout the early life of oysters. This study provides evidence of the biological significance of histone methylation during development in a lophotrochozoan species. Our results also indicate that temperature influences histone methylation, possibly through the expression level of putative actors of its regulation, which might participate in developmental control. To our knowledge, this is the first report indicating a direct relationship between an epigenetic mark and an environmental parameter in marine molluscs. Such investigations could help better understand the molecular mechanisms of development and adaptation in lophotrochozoans.

Authors+Show Affiliations

UMR BOREA Biologie des Organismes et Ecosystèmes Aquatiques, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, Institut de Biologie Fondamentale et Appliquée, Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen cedex 5, France.UMR BOREA Biologie des Organismes et Ecosystèmes Aquatiques, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, Institut de Biologie Fondamentale et Appliquée, Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen cedex 5, France.UMR BOREA Biologie des Organismes et Ecosystèmes Aquatiques, MNHN, UPMC, UCBN, CNRS-7208, IRD-207, Institut de Biologie Fondamentale et Appliquée, Université de Caen Basse-Normandie, Esplanade de la Paix, CS 14032, 14032 Caen cedex 5, France. Electronic address: guillaume.riviere@unicaen.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25224965

Citation

Fellous, Alexandre, et al. "Temperature Influences Histone Methylation and mRNA Expression of the Jmj-C Histone-demethylase Orthologues During the Early Development of the Oyster Crassostrea Gigas." Marine Genomics, vol. 19, 2015, pp. 23-30.
Fellous A, Favrel P, Riviere G. Temperature influences histone methylation and mRNA expression of the Jmj-C histone-demethylase orthologues during the early development of the oyster Crassostrea gigas. Mar Genomics. 2015;19:23-30.
Fellous, A., Favrel, P., & Riviere, G. (2015). Temperature influences histone methylation and mRNA expression of the Jmj-C histone-demethylase orthologues during the early development of the oyster Crassostrea gigas. Marine Genomics, 19, 23-30. https://doi.org/10.1016/j.margen.2014.09.002
Fellous A, Favrel P, Riviere G. Temperature Influences Histone Methylation and mRNA Expression of the Jmj-C Histone-demethylase Orthologues During the Early Development of the Oyster Crassostrea Gigas. Mar Genomics. 2015;19:23-30. PubMed PMID: 25224965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temperature influences histone methylation and mRNA expression of the Jmj-C histone-demethylase orthologues during the early development of the oyster Crassostrea gigas. AU - Fellous,Alexandre, AU - Favrel,Pascal, AU - Riviere,Guillaume, Y1 - 2014/09/16/ PY - 2014/03/26/received PY - 2014/09/03/revised PY - 2014/09/04/accepted PY - 2014/9/17/entrez PY - 2014/9/17/pubmed PY - 2015/12/15/medline KW - Development KW - Epigenetics KW - Histone methylation KW - Jumonji KW - Oyster KW - Temperature SP - 23 EP - 30 JF - Marine genomics JO - Mar Genomics VL - 19 N2 - In many groups, epigenetic mechanisms influence developmental gene regulation under environmental inputs. The Pacific oyster Crassostrea gigas belongs to lophotrochozoans and its larval development is highly dependent on temperature, but the role of epigenetic mechanisms in this context is unknown despite high levels of the recently characterized Jumonji histone demethylase (JHDM) orthologues (Cg_Jumonji) suggesting a physiological relevance of histone methylation in the oyster development. Because in other species alterations of the histone methylation pattern have deleterious outcomes, we investigated the influence of temperature during the oyster larval life on histone methylation and JHDM expression. To shed light on this point, oyster embryonic and early larval development experiments were carried out at different temperatures (18 °C, 25 °C and 32 °C). Histone methylation levels were investigated using fluorescent ELISA at 6 and 24h post-fertilization. When compared to the 25 °C group, at 18 °C H3K4, H3K9 and H3K27 residues were hypomethylated at 6h post fertilization (hpf) and hypermethylated at 24 hpf. In contrast, at 32 °C, 6hpf animals present a dramatic hypermethylation (ca. 4-fold) of all examined residues, which is minored but sustained at 24 hpf. RT-qPCR investigations of the mRNA expression of the nine oyster JHDMs, showed gene- and stage-specific temperature sensitivities throughout the early life of oysters. This study provides evidence of the biological significance of histone methylation during development in a lophotrochozoan species. Our results also indicate that temperature influences histone methylation, possibly through the expression level of putative actors of its regulation, which might participate in developmental control. To our knowledge, this is the first report indicating a direct relationship between an epigenetic mark and an environmental parameter in marine molluscs. Such investigations could help better understand the molecular mechanisms of development and adaptation in lophotrochozoans. SN - 1876-7478 UR - https://www.unboundmedicine.com/medline/citation/25224965/Temperature_influences_histone_methylation_and_mRNA_expression_of_the_Jmj_C_histone_demethylase_orthologues_during_the_early_development_of_the_oyster_Crassostrea_gigas_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-7787(14)00103-2 DB - PRIME DP - Unbound Medicine ER -