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Revealing metabolomic variations in Cortex Moutan from different root parts using HPLC-MS method.
Phytochem Anal. 2015 Jan-Feb; 26(1):86-93.PA

Abstract

INTRODUCTION

The distribution of metabolites in the different root parts of Cortex Moutan (the root bark of Paeonia suffruticosa Andrews) is not well understood, therefore, scientific evidence is not available for quality assessment of Cortex Moutan.

OBJECTIVE

To reveal metabolomic variations in Cortex Moutan in order to gain deeper insights to enable quality control.

METHODS

Metabolomic variations in the different root parts of Cortex Moutan were characterised using high-performance liquid chromatography combined with mass spectrometry (HPLC-MS) and multivariate data analysis. The discriminating metabolites in different root parts were evaluated by the one-way analysis of variance and a fold change parameter.

RESULTS

The metabolite profiles of Cortex Moutan were largely dominated by five primary and 41 secondary metabolites . Higher levels of malic acid, gallic acid and mudanoside-B were mainly observed in the second lateral roots, whereas dihydroxyacetophenone, benzoyloxypaeoniflorin, suffruticoside-A, kaempferol dihexoside, mudanpioside E and mudanpioside J accumulated in the first lateral and axial roots. The highest contents of paeonol, galloyloxypaeoniflorin and procyanidin B were detected in the axial roots. Accordingly, metabolite compositions of Cortex Moutan were found to vary among different root parts.

CONCLUSION

The axial roots have higher quality than the lateral roots in Cortex Moutan due to the accumulation of bioactive secondary metabolites associated with plant physiology. These findings provided important scientific evidence for grading Cortex Moutan on the general market.

Authors+Show Affiliations

College of Life Sciences, Northwest University, Xi'an, 710069, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25230378

Citation

Xiao, Chaoni, et al. "Revealing Metabolomic Variations in Cortex Moutan From Different Root Parts Using HPLC-MS Method." Phytochemical Analysis : PCA, vol. 26, no. 1, 2015, pp. 86-93.
Xiao C, Wu M, Chen Y, et al. Revealing metabolomic variations in Cortex Moutan from different root parts using HPLC-MS method. Phytochem Anal. 2015;26(1):86-93.
Xiao, C., Wu, M., Chen, Y., Zhang, Y., Zhao, X., & Zheng, X. (2015). Revealing metabolomic variations in Cortex Moutan from different root parts using HPLC-MS method. Phytochemical Analysis : PCA, 26(1), 86-93. https://doi.org/10.1002/pca.2539
Xiao C, et al. Revealing Metabolomic Variations in Cortex Moutan From Different Root Parts Using HPLC-MS Method. Phytochem Anal. 2015 Jan-Feb;26(1):86-93. PubMed PMID: 25230378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Revealing metabolomic variations in Cortex Moutan from different root parts using HPLC-MS method. AU - Xiao,Chaoni, AU - Wu,Man, AU - Chen,Yongyong, AU - Zhang,Yajun, AU - Zhao,Xinfeng, AU - Zheng,Xiaohui, Y1 - 2014/09/17/ PY - 2014/04/23/received PY - 2014/07/25/revised PY - 2014/08/03/accepted PY - 2014/9/18/entrez PY - 2014/9/18/pubmed PY - 2015/8/26/medline KW - Cortex Moutan KW - HPLC-MS KW - Paeonia suffruticosa KW - metabolomic SP - 86 EP - 93 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 26 IS - 1 N2 - INTRODUCTION: The distribution of metabolites in the different root parts of Cortex Moutan (the root bark of Paeonia suffruticosa Andrews) is not well understood, therefore, scientific evidence is not available for quality assessment of Cortex Moutan. OBJECTIVE: To reveal metabolomic variations in Cortex Moutan in order to gain deeper insights to enable quality control. METHODS: Metabolomic variations in the different root parts of Cortex Moutan were characterised using high-performance liquid chromatography combined with mass spectrometry (HPLC-MS) and multivariate data analysis. The discriminating metabolites in different root parts were evaluated by the one-way analysis of variance and a fold change parameter. RESULTS: The metabolite profiles of Cortex Moutan were largely dominated by five primary and 41 secondary metabolites . Higher levels of malic acid, gallic acid and mudanoside-B were mainly observed in the second lateral roots, whereas dihydroxyacetophenone, benzoyloxypaeoniflorin, suffruticoside-A, kaempferol dihexoside, mudanpioside E and mudanpioside J accumulated in the first lateral and axial roots. The highest contents of paeonol, galloyloxypaeoniflorin and procyanidin B were detected in the axial roots. Accordingly, metabolite compositions of Cortex Moutan were found to vary among different root parts. CONCLUSION: The axial roots have higher quality than the lateral roots in Cortex Moutan due to the accumulation of bioactive secondary metabolites associated with plant physiology. These findings provided important scientific evidence for grading Cortex Moutan on the general market. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/25230378/Revealing_metabolomic_variations_in_Cortex_Moutan_from_different_root_parts_using_HPLC_MS_method_ L2 - https://doi.org/10.1002/pca.2539 DB - PRIME DP - Unbound Medicine ER -