Tags

Type your tag names separated by a space and hit enter

Downregulation of miR-193a-5p correlates with lymph node metastasis and poor prognosis in colorectal cancer.
World J Gastroenterol. 2014 Sep 14; 20(34):12241-8.WJ

Abstract

AIM

To investigate the correlation of miR-193a-5p with lymph node metastasis and postoperative survival of colorectal cancer (CRC) patients.

METHODS

A total of 304 formalin-fixed, paraffin-embedded specimens (69 paired cancer and normal tissues, 55 primary tumors of stage III CRC and matched lymph nodes, and 56 primary tumors of stage II CRC) were included in this study. The relative expression levels of miR-193a-5p in the normal mucosa, primary cancer, and metastatic lymph node lesions were measured by quantitative real-time reverse transcriptase polymerase chain reaction. We evaluated the association of its expression with colorectal cancer lymph node metastasis, clinicopathological factors, and patient survival.

RESULTS

The relative expression level of miR-193a-5p was significantly lower in CRC tissues than in the normal mucosa (P = 0.0060). The expression levels of miR-193a-5p were lower in primary CRC tissues with lymph node metastases than in those without metastases (P = 0.0006), and decreased expression of miR-193a-5p correlated with advanced lymph node metastatic stage (P = 0.0007). Kaplan-Meier analysis showed that patients with low miR-193a-5p expression had decreased disease-free survival (DFS) (P = 0.0026) and poor overall survival (OS) (P = 0.0003). Interestingly, for the group of patients with lymph node metastases, miR-193a-5p expression was also related to survival. Patients with low miR-193a-5p expression had decreased DFS (P = 0.0262) and poor OS (P = 0.0230). Moreover, multivariate analysis indicated that downregulation of miR-193a-5p was an independent predictor of poor OS.

CONCLUSION

Downregulation of miR-193a-5p correlates with lymph node metastasis and poor survival of CRC. miR-193a-5p may be a useful biomarker for CRC diagnosis, metastasis and prognosis prediction.

Authors+Show Affiliations

Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.Peng Zhang, Deng-Bo Ji, Chang-Zheng Du, Jin Gu, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Colorectal Surgery, Peking University Cancer Hospital and Institute, Beijing 100142, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25232258

Citation

Zhang, Peng, et al. "Downregulation of miR-193a-5p Correlates With Lymph Node Metastasis and Poor Prognosis in Colorectal Cancer." World Journal of Gastroenterology, vol. 20, no. 34, 2014, pp. 12241-8.
Zhang P, Ji DB, Han HB, et al. Downregulation of miR-193a-5p correlates with lymph node metastasis and poor prognosis in colorectal cancer. World J Gastroenterol. 2014;20(34):12241-8.
Zhang, P., Ji, D. B., Han, H. B., Shi, Y. F., Du, C. Z., & Gu, J. (2014). Downregulation of miR-193a-5p correlates with lymph node metastasis and poor prognosis in colorectal cancer. World Journal of Gastroenterology, 20(34), 12241-8. https://doi.org/10.3748/wjg.v20.i34.12241
Zhang P, et al. Downregulation of miR-193a-5p Correlates With Lymph Node Metastasis and Poor Prognosis in Colorectal Cancer. World J Gastroenterol. 2014 Sep 14;20(34):12241-8. PubMed PMID: 25232258.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Downregulation of miR-193a-5p correlates with lymph node metastasis and poor prognosis in colorectal cancer. AU - Zhang,Peng, AU - Ji,Deng-Bo, AU - Han,Hai-Bo, AU - Shi,Yun-Fei, AU - Du,Chang-Zheng, AU - Gu,Jin, PY - 2014/03/14/received PY - 2014/05/08/revised PY - 2014/05/29/accepted PY - 2014/9/19/entrez PY - 2014/9/19/pubmed PY - 2015/5/16/medline KW - Biomarker KW - Colorectal cancer KW - Lymph node metastasis KW - Prognosis KW - miR-193a-5p SP - 12241 EP - 8 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 20 IS - 34 N2 - AIM: To investigate the correlation of miR-193a-5p with lymph node metastasis and postoperative survival of colorectal cancer (CRC) patients. METHODS: A total of 304 formalin-fixed, paraffin-embedded specimens (69 paired cancer and normal tissues, 55 primary tumors of stage III CRC and matched lymph nodes, and 56 primary tumors of stage II CRC) were included in this study. The relative expression levels of miR-193a-5p in the normal mucosa, primary cancer, and metastatic lymph node lesions were measured by quantitative real-time reverse transcriptase polymerase chain reaction. We evaluated the association of its expression with colorectal cancer lymph node metastasis, clinicopathological factors, and patient survival. RESULTS: The relative expression level of miR-193a-5p was significantly lower in CRC tissues than in the normal mucosa (P = 0.0060). The expression levels of miR-193a-5p were lower in primary CRC tissues with lymph node metastases than in those without metastases (P = 0.0006), and decreased expression of miR-193a-5p correlated with advanced lymph node metastatic stage (P = 0.0007). Kaplan-Meier analysis showed that patients with low miR-193a-5p expression had decreased disease-free survival (DFS) (P = 0.0026) and poor overall survival (OS) (P = 0.0003). Interestingly, for the group of patients with lymph node metastases, miR-193a-5p expression was also related to survival. Patients with low miR-193a-5p expression had decreased DFS (P = 0.0262) and poor OS (P = 0.0230). Moreover, multivariate analysis indicated that downregulation of miR-193a-5p was an independent predictor of poor OS. CONCLUSION: Downregulation of miR-193a-5p correlates with lymph node metastasis and poor survival of CRC. miR-193a-5p may be a useful biomarker for CRC diagnosis, metastasis and prognosis prediction. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/25232258/Downregulation_of_miR_193a_5p_correlates_with_lymph_node_metastasis_and_poor_prognosis_in_colorectal_cancer_ L2 - http://www.wjgnet.com/1007-9327/full/v20/i34/12241.htm DB - PRIME DP - Unbound Medicine ER -