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Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column.
Food Chem. 2015 Feb 15; 169:169-79.FC

Abstract

A reversed-phase high-performance liquid chromatography method with photodiode array detection has been developed enabling the joint determination of 17 prominent flavonoids and phenolic acids in vegetables and fruits. A multi-segmented gradient program using a fused-core column for the separation of several phenolic classes (phenolic acids and flavonoids) has been optimised. The influence of extraction conditions (sample freeze-drying, ultrasound extraction, solvent composition and extraction time) has been also optimised using response surface methodology with tomato samples as a model. Complete recoveries (76-108%) were obtained for the phenolic compounds present in tomato. The developed method provided satisfactory repeatability in terms of peak area (RSD<2.9%) and retention time (RSD<0.2%) both for standards and real samples. Detection limits ranged between 3 and 44μgkg(-1) for the detected polyphenols. This method is recommended for routine analysis of large number of samples typical of production quality systems or plant breeding programs.

Authors+Show Affiliations

Department de Ciències Agràries i del Medi Natural, Universitat Jaume I, Avda. Sos Baynat s/n, 12071 Castellón, Spain.Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universitat Politècnica de València, Cno. de Vera s/n, 46022 València, Spain.Departamento de Química Analítica, Universitat de València, Dr. Moliner, 50, 46100 Burjassot, Spain.Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universitat Politècnica de València, Cno. de Vera s/n, 46022 València, Spain.Department de Ciències Agràries i del Medi Natural, Universitat Jaume I, Avda. Sos Baynat s/n, 12071 Castellón, Spain. Electronic address: rosello@uji.es.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25236213

Citation

Martí, Raúl, et al. "Fast Simultaneous Determination of Prominent Polyphenols in Vegetables and Fruits By Reversed Phase Liquid Chromatography Using a Fused-core Column." Food Chemistry, vol. 169, 2015, pp. 169-79.
Martí R, Valcárcel M, Herrero-Martínez JM, et al. Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column. Food Chem. 2015;169:169-79.
Martí, R., Valcárcel, M., Herrero-Martínez, J. M., Cebolla-Cornejo, J., & Roselló, S. (2015). Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column. Food Chemistry, 169, 169-79. https://doi.org/10.1016/j.foodchem.2014.07.151
Martí R, et al. Fast Simultaneous Determination of Prominent Polyphenols in Vegetables and Fruits By Reversed Phase Liquid Chromatography Using a Fused-core Column. Food Chem. 2015 Feb 15;169:169-79. PubMed PMID: 25236213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column. AU - Martí,Raúl, AU - Valcárcel,Mercedes, AU - Herrero-Martínez,José Manuel, AU - Cebolla-Cornejo,Jaime, AU - Roselló,Salvador, Y1 - 2014/08/07/ PY - 2014/04/09/received PY - 2014/07/16/revised PY - 2014/07/30/accepted PY - 2014/9/20/entrez PY - 2014/9/23/pubmed PY - 2015/11/17/medline KW - Food KW - Functional quality KW - HPLC-DAD KW - Response surface methodology KW - Ultrasound-assisted extraction SP - 169 EP - 79 JF - Food chemistry JO - Food Chem VL - 169 N2 - A reversed-phase high-performance liquid chromatography method with photodiode array detection has been developed enabling the joint determination of 17 prominent flavonoids and phenolic acids in vegetables and fruits. A multi-segmented gradient program using a fused-core column for the separation of several phenolic classes (phenolic acids and flavonoids) has been optimised. The influence of extraction conditions (sample freeze-drying, ultrasound extraction, solvent composition and extraction time) has been also optimised using response surface methodology with tomato samples as a model. Complete recoveries (76-108%) were obtained for the phenolic compounds present in tomato. The developed method provided satisfactory repeatability in terms of peak area (RSD<2.9%) and retention time (RSD<0.2%) both for standards and real samples. Detection limits ranged between 3 and 44μgkg(-1) for the detected polyphenols. This method is recommended for routine analysis of large number of samples typical of production quality systems or plant breeding programs. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/25236213/Fast_simultaneous_determination_of_prominent_polyphenols_in_vegetables_and_fruits_by_reversed_phase_liquid_chromatography_using_a_fused_core_column_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(14)01199-6 DB - PRIME DP - Unbound Medicine ER -