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Effect of light, methyl jasmonate and cyclodextrin on production of phenolic compounds in hairy root cultures of Scutellaria lateriflora.
Phytochemistry. 2014 Nov; 107:50-60.P

Abstract

Scutellaria lateriflora (American skullcap) has been used in traditional medicine to treat several medical conditions including nervous disorders and cancer. Previous studies have associated these medicinal properties to flavones present in roots and leaves of this species. In order to develop a production system and study the biosynthesis of these bioactive compounds, hairy root cultures of S. lateriflora were established and line 4 was selected for further studies based on its growth performance in a modified Murashige and Skoog's medium supplemented with 0.5mg/l indole-3-butyric acid. Scanning electron microscopy of the hairy roots showed a high profusion of hairs along the root. Several phenolic compounds, including verbascoside, and the flavones wogonin, baicalein, scutellarein and their respective glucuronides were identified by high performance liquid chromatography-tandem mass spectrometry in the root tissue, but not in the culture medium. Among these compounds, verbascoside accumulated at the highest levels. Interestingly, cultures incubated under continuous light and treated with 15mM methyl-β-cyclodextrin for 24h produced significantly higher levels of the aglycones, baicalein and wogonin, but not scutellarein, compared to cultures incubated under continuous darkness. This work demonstrates that hairy root cultures of S. lateriflora have the biosynthetic capacity to produce known Scutellaria flavones and suggest that light may have a selected regulatory effect on the synthesis or accumulation of these phenolic compounds.

Authors+Show Affiliations

Arkansas Biosciences Institute, Arkansas State University, State University, AR 72467, USA; Department of Biological Sciences, Arkansas State University, State University, AR 72467, USA.Arkansas Biosciences Institute, Arkansas State University, State University, AR 72467, USA; Department of Biological Sciences, Arkansas State University, State University, AR 72467, USA.Arkansas Biosciences Institute, Arkansas State University, State University, AR 72467, USA; Department of Biological Sciences, Arkansas State University, State University, AR 72467, USA.Arkansas Biosciences Institute, Arkansas State University, State University, AR 72467, USA.Arkansas Biosciences Institute, Arkansas State University, State University, AR 72467, USA.Agricultural Research Station, Fort Valley State University, Fort Valley, GA 31030, USA.Arkansas Biosciences Institute, Arkansas State University, State University, AR 72467, USA; Department of Biological Sciences, Arkansas State University, State University, AR 72467, USA. Electronic address: fmedinabolivar@astate.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25236693

Citation

Marsh, Zachary, et al. "Effect of Light, Methyl Jasmonate and Cyclodextrin On Production of Phenolic Compounds in Hairy Root Cultures of Scutellaria Lateriflora." Phytochemistry, vol. 107, 2014, pp. 50-60.
Marsh Z, Yang T, Nopo-Olazabal L, et al. Effect of light, methyl jasmonate and cyclodextrin on production of phenolic compounds in hairy root cultures of Scutellaria lateriflora. Phytochemistry. 2014;107:50-60.
Marsh, Z., Yang, T., Nopo-Olazabal, L., Wu, S., Ingle, T., Joshee, N., & Medina-Bolivar, F. (2014). Effect of light, methyl jasmonate and cyclodextrin on production of phenolic compounds in hairy root cultures of Scutellaria lateriflora. Phytochemistry, 107, 50-60. https://doi.org/10.1016/j.phytochem.2014.08.020
Marsh Z, et al. Effect of Light, Methyl Jasmonate and Cyclodextrin On Production of Phenolic Compounds in Hairy Root Cultures of Scutellaria Lateriflora. Phytochemistry. 2014;107:50-60. PubMed PMID: 25236693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of light, methyl jasmonate and cyclodextrin on production of phenolic compounds in hairy root cultures of Scutellaria lateriflora. AU - Marsh,Zachary, AU - Yang,Tianhong, AU - Nopo-Olazabal,Luis, AU - Wu,Shuchi, AU - Ingle,Taylor, AU - Joshee,Nirmal, AU - Medina-Bolivar,Fabricio, Y1 - 2014/09/15/ PY - 2013/12/12/received PY - 2014/05/17/revised PY - 2014/05/17/accepted PY - 2014/9/20/entrez PY - 2014/9/23/pubmed PY - 2015/2/5/medline KW - Baicalein KW - Cyclodextrin KW - Elicitation KW - Hairy roots KW - Lamiaceae KW - Methyl jasmonate KW - Phenolics KW - Scutellarein KW - Scutellaria KW - Verbascoside KW - Wogonin SP - 50 EP - 60 JF - Phytochemistry JO - Phytochemistry VL - 107 N2 - Scutellaria lateriflora (American skullcap) has been used in traditional medicine to treat several medical conditions including nervous disorders and cancer. Previous studies have associated these medicinal properties to flavones present in roots and leaves of this species. In order to develop a production system and study the biosynthesis of these bioactive compounds, hairy root cultures of S. lateriflora were established and line 4 was selected for further studies based on its growth performance in a modified Murashige and Skoog's medium supplemented with 0.5mg/l indole-3-butyric acid. Scanning electron microscopy of the hairy roots showed a high profusion of hairs along the root. Several phenolic compounds, including verbascoside, and the flavones wogonin, baicalein, scutellarein and their respective glucuronides were identified by high performance liquid chromatography-tandem mass spectrometry in the root tissue, but not in the culture medium. Among these compounds, verbascoside accumulated at the highest levels. Interestingly, cultures incubated under continuous light and treated with 15mM methyl-β-cyclodextrin for 24h produced significantly higher levels of the aglycones, baicalein and wogonin, but not scutellarein, compared to cultures incubated under continuous darkness. This work demonstrates that hairy root cultures of S. lateriflora have the biosynthetic capacity to produce known Scutellaria flavones and suggest that light may have a selected regulatory effect on the synthesis or accumulation of these phenolic compounds. SN - 1873-3700 UR - https://www.unboundmedicine.com/medline/citation/25236693/Effect_of_light_methyl_jasmonate_and_cyclodextrin_on_production_of_phenolic_compounds_in_hairy_root_cultures_of_Scutellaria_lateriflora_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0031-9422(14)00354-9 DB - PRIME DP - Unbound Medicine ER -