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Antimicrobial susceptibility patterns of Clostridium difficile strains belonging to different polymerase chain reaction ribotypes isolated in Poland in 2012.
Anaerobe. 2015 Feb; 31:37-41.A

Abstract

In the beginning of 2012, a study was conducted to obtain an overview of Clostridium difficile infections (CDIs) in Polish hospitals. The collection of 83 toxigenic C. difficile isolates obtained from this hospital-based survey was used to identify antimicrobial susceptibility patterns. Among the C. difficile isolates analyzed, 48 (57.8%) belonged to PCR ribotype 027, 21 (25.3%) to its closely related PCR ribotype 176, and 14 (16.9%) to different PCR ribotypes. Seventy one (85.5%) isolates were resistant to erythromycin, whereas 23 (27.7%) had high-level clindamycin resistance, having minimum inhibitory concentrations (MICs) greater than 256 mg/L. All strains were ciprofloxacin resistant and 69 (83.1%) were moxifloxacin resistant. Seventy-three (87.9%) strains were imipenem resistant, but only 2 (2.4%) strains were resistant to tetracycline. All strains were sensitive to tigecycline. Metronidazole and vancomycin were generally effective against the C. difficile isolates, both having an MIC90 value of 0.75 mg/L. Isolates belonging to PCR ribotype 027 and the closely related PCR ribotype 176, showed higher resistance. All ribotype 027 and 176 C. difficile isolates demonstrated high-level resistance to erythromycin (MIC ≥ 256 mg/L), and 95,2% of ribotype 176 isolates were co-resistant to erythromycin and clindamycin. The MIC of moxifloxacin for this epidemic strain was very high (≥32 mg/L). Resistance to erythromycin, moxifloxacin, and rifampicin was observed in 15 (18%) of the isolates, all of which belonged to PCR ribotype 027. Multidrug resistance (MDR), defined as resistance at least to three classes of antimicrobial agents was observed in 85.5% (n = 71) of toxigenic C. difficile strains.

Authors+Show Affiliations

Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland.Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland. Electronic address: hanna.pituch@wum.edu.pl.Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25242196

Citation

Lachowicz, Dominika, et al. "Antimicrobial Susceptibility Patterns of Clostridium Difficile Strains Belonging to Different Polymerase Chain Reaction Ribotypes Isolated in Poland in 2012." Anaerobe, vol. 31, 2015, pp. 37-41.
Lachowicz D, Pituch H, Obuch-Woszczatyński P. Antimicrobial susceptibility patterns of Clostridium difficile strains belonging to different polymerase chain reaction ribotypes isolated in Poland in 2012. Anaerobe. 2015;31:37-41.
Lachowicz, D., Pituch, H., & Obuch-Woszczatyński, P. (2015). Antimicrobial susceptibility patterns of Clostridium difficile strains belonging to different polymerase chain reaction ribotypes isolated in Poland in 2012. Anaerobe, 31, 37-41. https://doi.org/10.1016/j.anaerobe.2014.09.004
Lachowicz D, Pituch H, Obuch-Woszczatyński P. Antimicrobial Susceptibility Patterns of Clostridium Difficile Strains Belonging to Different Polymerase Chain Reaction Ribotypes Isolated in Poland in 2012. Anaerobe. 2015;31:37-41. PubMed PMID: 25242196.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial susceptibility patterns of Clostridium difficile strains belonging to different polymerase chain reaction ribotypes isolated in Poland in 2012. AU - Lachowicz,Dominika, AU - Pituch,Hanna, AU - Obuch-Woszczatyński,Piotr, Y1 - 2014/09/19/ PY - 2014/06/30/received PY - 2014/08/26/revised PY - 2014/09/04/accepted PY - 2014/9/23/entrez PY - 2014/9/23/pubmed PY - 2015/10/29/medline KW - Clostridium difficile KW - Multidrugs resistance KW - PCR ribotype 027 and 176 KW - Susceptibility to antimicrobial drugs SP - 37 EP - 41 JF - Anaerobe JO - Anaerobe VL - 31 N2 - In the beginning of 2012, a study was conducted to obtain an overview of Clostridium difficile infections (CDIs) in Polish hospitals. The collection of 83 toxigenic C. difficile isolates obtained from this hospital-based survey was used to identify antimicrobial susceptibility patterns. Among the C. difficile isolates analyzed, 48 (57.8%) belonged to PCR ribotype 027, 21 (25.3%) to its closely related PCR ribotype 176, and 14 (16.9%) to different PCR ribotypes. Seventy one (85.5%) isolates were resistant to erythromycin, whereas 23 (27.7%) had high-level clindamycin resistance, having minimum inhibitory concentrations (MICs) greater than 256 mg/L. All strains were ciprofloxacin resistant and 69 (83.1%) were moxifloxacin resistant. Seventy-three (87.9%) strains were imipenem resistant, but only 2 (2.4%) strains were resistant to tetracycline. All strains were sensitive to tigecycline. Metronidazole and vancomycin were generally effective against the C. difficile isolates, both having an MIC90 value of 0.75 mg/L. Isolates belonging to PCR ribotype 027 and the closely related PCR ribotype 176, showed higher resistance. All ribotype 027 and 176 C. difficile isolates demonstrated high-level resistance to erythromycin (MIC ≥ 256 mg/L), and 95,2% of ribotype 176 isolates were co-resistant to erythromycin and clindamycin. The MIC of moxifloxacin for this epidemic strain was very high (≥32 mg/L). Resistance to erythromycin, moxifloxacin, and rifampicin was observed in 15 (18%) of the isolates, all of which belonged to PCR ribotype 027. Multidrug resistance (MDR), defined as resistance at least to three classes of antimicrobial agents was observed in 85.5% (n = 71) of toxigenic C. difficile strains. SN - 1095-8274 UR - https://www.unboundmedicine.com/medline/citation/25242196/Antimicrobial_susceptibility_patterns_of_Clostridium_difficile_strains_belonging_to_different_polymerase_chain_reaction_ribotypes_isolated_in_Poland_in_2012_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1075-9964(14)00125-5 DB - PRIME DP - Unbound Medicine ER -