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Carum copticum and Thymus vulgaris oils inhibit virulence in Trichophyton rubrum and Aspergillus spp.
Braz J Microbiol. 2014; 45(2):523-31.BJ

Abstract

Emergence of drug-resistant strains has demanded for alternative means of combating fungal infections. Oils of Carum copticum and Thymus vulgaris have long been used in ethnomedicine for ailments of various fungal infections. Since their activity has not been reported in particular against drug-resistant fungi, this study was aimed to evaluate the effects of oils of C. copticum and T. vulgaris on the growth and virulence of drug-resistant strains of Aspergillus spp. and Trichophyton rubrum. The gas chromatography-mass spectrometry analysis revealed thymol constituting 44.71% and 22.82% of T. vulgaris and C. copticum, respectively. Inhibition of mycelial growth by essential oils was recorded in the order of thymol > T. vulgaris > C. copticum against the tested strains. RBC lysis assay showed no tested oils to be toxic even up to concentration two folds higher than their respective MFCs. Thymol exhibited highest synergy in combination with fluconazole against Aspergillus fumigatus MTCC2550 (FICI value 0.187) and T. rubrum IOA9 (0.156) as determined by checkerboard method. Thymol and T. vulgaris essential oil were equally effective against both the macro and arthroconidia growth (MIC 72 μg/mL). A > 80% reduction in elastase activity was recorded for A. fumigatus MTCC2550 by C. copticum, T. vulgaris oils and thymol. The effectiveness of these oils against arthroconidia and synergistic interaction of thymol and T. vulgaris with fluconazole can be exploited to potentiate the antifungal effects of fluconazole against drug-resistant strains of T. rubrum and Aspergillus spp.

Authors+Show Affiliations

Department of Agricultural Microbiology Aligarh Muslim University Aligarh India Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh, India. ; Institute of Microbial Technology Chandigarh India Institute of Microbial Technology, Chandigarh, India.Department of Agricultural Microbiology Aligarh Muslim University Aligarh India Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh, India.Institute of Microbial Technology Chandigarh India Institute of Microbial Technology, Chandigarh, India.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25242937

Citation

Khan, Mohd Sajjad Ahmad, et al. "Carum Copticum and Thymus Vulgaris Oils Inhibit Virulence in Trichophyton Rubrum and Aspergillus Spp." Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology], vol. 45, no. 2, 2014, pp. 523-31.
Khan MS, Ahmad I, Cameotra SS. Carum copticum and Thymus vulgaris oils inhibit virulence in Trichophyton rubrum and Aspergillus spp. Braz J Microbiol. 2014;45(2):523-31.
Khan, M. S., Ahmad, I., & Cameotra, S. S. (2014). Carum copticum and Thymus vulgaris oils inhibit virulence in Trichophyton rubrum and Aspergillus spp. Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology], 45(2), 523-31.
Khan MS, Ahmad I, Cameotra SS. Carum Copticum and Thymus Vulgaris Oils Inhibit Virulence in Trichophyton Rubrum and Aspergillus Spp. Braz J Microbiol. 2014;45(2):523-31. PubMed PMID: 25242937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carum copticum and Thymus vulgaris oils inhibit virulence in Trichophyton rubrum and Aspergillus spp. AU - Khan,Mohd Sajjad Ahmad, AU - Ahmad,Iqbal, AU - Cameotra,Swaranjit Singh, Y1 - 2014/08/29/ PY - 2012/08/17/received PY - 2013/09/09/accepted PY - 2014/9/23/entrez PY - 2014/9/23/pubmed PY - 2015/5/29/medline KW - anti-elastase activity KW - arthroconidia KW - synergy KW - thymol KW - virulence SP - 523 EP - 31 JF - Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] JO - Braz J Microbiol VL - 45 IS - 2 N2 - Emergence of drug-resistant strains has demanded for alternative means of combating fungal infections. Oils of Carum copticum and Thymus vulgaris have long been used in ethnomedicine for ailments of various fungal infections. Since their activity has not been reported in particular against drug-resistant fungi, this study was aimed to evaluate the effects of oils of C. copticum and T. vulgaris on the growth and virulence of drug-resistant strains of Aspergillus spp. and Trichophyton rubrum. The gas chromatography-mass spectrometry analysis revealed thymol constituting 44.71% and 22.82% of T. vulgaris and C. copticum, respectively. Inhibition of mycelial growth by essential oils was recorded in the order of thymol > T. vulgaris > C. copticum against the tested strains. RBC lysis assay showed no tested oils to be toxic even up to concentration two folds higher than their respective MFCs. Thymol exhibited highest synergy in combination with fluconazole against Aspergillus fumigatus MTCC2550 (FICI value 0.187) and T. rubrum IOA9 (0.156) as determined by checkerboard method. Thymol and T. vulgaris essential oil were equally effective against both the macro and arthroconidia growth (MIC 72 μg/mL). A > 80% reduction in elastase activity was recorded for A. fumigatus MTCC2550 by C. copticum, T. vulgaris oils and thymol. The effectiveness of these oils against arthroconidia and synergistic interaction of thymol and T. vulgaris with fluconazole can be exploited to potentiate the antifungal effects of fluconazole against drug-resistant strains of T. rubrum and Aspergillus spp. SN - 1678-4405 UR - https://www.unboundmedicine.com/medline/citation/25242937/Carum_copticum_and_Thymus_vulgaris_oils_inhibit_virulence_in_Trichophyton_rubrum_and_Aspergillus_spp_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25242937/ DB - PRIME DP - Unbound Medicine ER -