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Antioxidative and chemopreventive effects of Nephrolepis biserrata against carbon tetrachloride (CCl4)-induced oxidative stress and hepatic dysfunction in rats.
Pharm Biol. 2015 Jan; 53(1):31-9.PB

Abstract

CONTEXT

Nephrolepis biserrata L. (Nephrolepidaceae) has been used in folk medicine for protection against different diseases.

OBJECTIVE

The current research investigated the protective effect of the methanol extract of N. biserrata leaves against carbon tetrachloride (CCl4)-induced hepatic damage in rats.

MATERIALS AND METHODS

Total phenolic content and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were estimated. In addition, Sprague-Dawley (SD) rats were randomly divided into six groups: control, CCl4 (1.0 mg/kg b wt), N. biserrata extract (at doses of 125, 250, and 375 mg/kg b wt) with CCl4 and N. biserrata extract (at dose of 375 mg/kg b wt) alone. After 2 weeks all rats were sacrificed and hepatoprotective effect of N. biserrata was evaluated.

RESULTS

Our results indicated that the high total phenolic content (127.28 ± 1.57 mg GAE/g) of N. biserrata may be the major contributor to strong antioxidant activities. Moreover, N. biserrata significantly depleted the elevation of enzymatic levels of alanine aminotransferase and aspartate aminotransferase (20-93% recovery), reduced the extent of malondialdehyde (47-90% recovery), increased the level of reduced glutathione (25-39% recovery), and elevated the activities of catalase, glutathione reductase, glutathione peroxidase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and quinone reductase (5-34% recovery). Histopathological observations also revealed that N. biserrata decreased fatty degeneration and necrosis in CCl4 administered rats.

DISCUSSION AND CONCLUSION

N. biserrata has strong antioxidant activities and significant protective effects against CCl4 induced hepatotoxicity in rats.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Shah MD
Biotechnology Research Institute, Universiti Malaysia Sabah , Jalan UMS, Kota Kinabalu, Sabah , Malaysia and.
Gnanaraj C
No affiliation info available
Haque AT
No affiliation info available
Iqbal M
No affiliation info available

MeSH

Administration, OralAnimalsAntioxidantsCarbon TetrachlorideChemical and Drug Induced Liver InjuryDose-Response Relationship, DrugLipid PeroxidationLiverLiver Function TestsMaleOxidative StressPlant ExtractsPlant LeavesPolypodiaceaeRandom AllocationRats, Sprague-Dawley

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25243876

Citation

Shah, Muhammad Dawood, et al. "Antioxidative and Chemopreventive Effects of Nephrolepis Biserrata Against Carbon Tetrachloride (CCl4)-induced Oxidative Stress and Hepatic Dysfunction in Rats." Pharmaceutical Biology, vol. 53, no. 1, 2015, pp. 31-9.
Shah MD, Gnanaraj C, Haque AT, et al. Antioxidative and chemopreventive effects of Nephrolepis biserrata against carbon tetrachloride (CCl4)-induced oxidative stress and hepatic dysfunction in rats. Pharm Biol. 2015;53(1):31-9.
Shah, M. D., Gnanaraj, C., Haque, A. T., & Iqbal, M. (2015). Antioxidative and chemopreventive effects of Nephrolepis biserrata against carbon tetrachloride (CCl4)-induced oxidative stress and hepatic dysfunction in rats. Pharmaceutical Biology, 53(1), 31-9. https://doi.org/10.3109/13880209.2014.909502
Shah MD, et al. Antioxidative and Chemopreventive Effects of Nephrolepis Biserrata Against Carbon Tetrachloride (CCl4)-induced Oxidative Stress and Hepatic Dysfunction in Rats. Pharm Biol. 2015;53(1):31-9. PubMed PMID: 25243876.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidative and chemopreventive effects of Nephrolepis biserrata against carbon tetrachloride (CCl4)-induced oxidative stress and hepatic dysfunction in rats. AU - Shah,Muhammad Dawood, AU - Gnanaraj,Charles, AU - Haque,A T M Emdadul, AU - Iqbal,Mohammad, Y1 - 2014/09/22/ PY - 2014/9/23/entrez PY - 2014/9/23/pubmed PY - 2015/9/4/medline KW - Antioxidant enzymes KW - histopathology KW - liver injury KW - methanol extract KW - oxidative stress KW - total phenolic content SP - 31 EP - 9 JF - Pharmaceutical biology JO - Pharm Biol VL - 53 IS - 1 N2 - CONTEXT: Nephrolepis biserrata L. (Nephrolepidaceae) has been used in folk medicine for protection against different diseases. OBJECTIVE: The current research investigated the protective effect of the methanol extract of N. biserrata leaves against carbon tetrachloride (CCl4)-induced hepatic damage in rats. MATERIALS AND METHODS: Total phenolic content and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were estimated. In addition, Sprague-Dawley (SD) rats were randomly divided into six groups: control, CCl4 (1.0 mg/kg b wt), N. biserrata extract (at doses of 125, 250, and 375 mg/kg b wt) with CCl4 and N. biserrata extract (at dose of 375 mg/kg b wt) alone. After 2 weeks all rats were sacrificed and hepatoprotective effect of N. biserrata was evaluated. RESULTS: Our results indicated that the high total phenolic content (127.28 ± 1.57 mg GAE/g) of N. biserrata may be the major contributor to strong antioxidant activities. Moreover, N. biserrata significantly depleted the elevation of enzymatic levels of alanine aminotransferase and aspartate aminotransferase (20-93% recovery), reduced the extent of malondialdehyde (47-90% recovery), increased the level of reduced glutathione (25-39% recovery), and elevated the activities of catalase, glutathione reductase, glutathione peroxidase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and quinone reductase (5-34% recovery). Histopathological observations also revealed that N. biserrata decreased fatty degeneration and necrosis in CCl4 administered rats. DISCUSSION AND CONCLUSION: N. biserrata has strong antioxidant activities and significant protective effects against CCl4 induced hepatotoxicity in rats. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25243876/Antioxidative_and_chemopreventive_effects_of_Nephrolepis_biserrata_against_carbon_tetrachloride__CCl4__induced_oxidative_stress_and_hepatic_dysfunction_in_rats_ DB - PRIME DP - Unbound Medicine ER -