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Theaflavins inhibit pathogenic properties of P. gingivalis and MMPs production in P. gingivalis-stimulated human gingival fibroblasts.
Arch Oral Biol. 2015 Jan; 60(1):12-22.AO

Abstract

OBJECTIVES

Theaflavins, the main polyphenols in black tea, possesses a wide range of beneficial pharmacological properties. Porphyromonas gingivalis (P. gingivalis) is a major aetiological agent associated with periodontitis, a chronic inflammatory disease affecting tooth-supporting tissues. The aim of the present study is to investigate the effect of theaflavins on pathogenic properties of P. gingivalis and on periodontitis by inhibiting matrix metalloproteinases (MMPs) production induced by this oral pathogen.

METHODS

Microplate dilution assays were performed to determine the effect of theaflavins against planktonic culture and biofilm of P. gingivalis. The effect of theaflavins on gingipain and collagenase activities of P. gingivalis was evaluated using synthetic chromogenic peptides and fluorogenic substrate. Human gingival fibroblasts (HGFs) were stimulated with P. gingivalis in the presence or absence of theaflavins, and then MMP-1, -2 secretion and their mRNA expression were assessed using an enzyme-linked immunosorbent assay (ELISA) and real-time PCR analysis, respectively.

RESULTS

Theaflavins exhibited the antimicrobial effects against both planktonic culture and biofilm of P. gingivalis. Theaflavins also markedly inhibited the proteinase activities of P. gingivalis collagenase and gingipains in a dose-dependent manner. Lastly, theaflavins significantly inhibited the secretion and mRNA expression of MMP-1 and MMP-2 by HGFs stimulated with P. gingivalis.

CONCLUSION

Theaflavins can affect the virulent properties of P. gingivalis and attenuate the MMP-mediated inflammatory response induced by this pathogen, which suggests that theaflavins may be potentially valuable supplementary therapeutic agent for prevention and treatment of P. gingivalis-associated periodontal diseases.

Authors+Show Affiliations

Department of Periodontics and Oral Mucosa, Jinan Stomatologic Hospital, No. 101 Jingliu Road, Jinan, Shandong, China; State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, Sichuan, China.Department of Periodontics, Hebei medical University, Shijiazhuang, Hebei, China.State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, Sichuan, China.State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, Sichuan, China.Department of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.Department of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China. Electronic address: jollyzldoc@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25244614

Citation

Kong, Lingxue, et al. "Theaflavins Inhibit Pathogenic Properties of P. Gingivalis and MMPs Production in P. Gingivalis-stimulated Human Gingival Fibroblasts." Archives of Oral Biology, vol. 60, no. 1, 2015, pp. 12-22.
Kong L, Qi X, Huang S, et al. Theaflavins inhibit pathogenic properties of P. gingivalis and MMPs production in P. gingivalis-stimulated human gingival fibroblasts. Arch Oral Biol. 2015;60(1):12-22.
Kong, L., Qi, X., Huang, S., Chen, S., Wu, Y., & Zhao, L. (2015). Theaflavins inhibit pathogenic properties of P. gingivalis and MMPs production in P. gingivalis-stimulated human gingival fibroblasts. Archives of Oral Biology, 60(1), 12-22. https://doi.org/10.1016/j.archoralbio.2014.08.019
Kong L, et al. Theaflavins Inhibit Pathogenic Properties of P. Gingivalis and MMPs Production in P. Gingivalis-stimulated Human Gingival Fibroblasts. Arch Oral Biol. 2015;60(1):12-22. PubMed PMID: 25244614.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Theaflavins inhibit pathogenic properties of P. gingivalis and MMPs production in P. gingivalis-stimulated human gingival fibroblasts. AU - Kong,Lingxue, AU - Qi,Xia, AU - Huang,Shiyan, AU - Chen,Shanshan, AU - Wu,Yafei, AU - Zhao,Lei, Y1 - 2014/09/10/ PY - 2014/02/11/received PY - 2014/07/30/revised PY - 2014/08/30/accepted PY - 2014/9/23/entrez PY - 2014/9/23/pubmed PY - 2015/8/19/medline KW - Gingipain KW - Human gingival fibroblasts KW - Matrix metalloproteinases KW - Periodontal disease KW - Porphyromonas gingivalis KW - Theaflavins SP - 12 EP - 22 JF - Archives of oral biology JO - Arch Oral Biol VL - 60 IS - 1 N2 - OBJECTIVES: Theaflavins, the main polyphenols in black tea, possesses a wide range of beneficial pharmacological properties. Porphyromonas gingivalis (P. gingivalis) is a major aetiological agent associated with periodontitis, a chronic inflammatory disease affecting tooth-supporting tissues. The aim of the present study is to investigate the effect of theaflavins on pathogenic properties of P. gingivalis and on periodontitis by inhibiting matrix metalloproteinases (MMPs) production induced by this oral pathogen. METHODS: Microplate dilution assays were performed to determine the effect of theaflavins against planktonic culture and biofilm of P. gingivalis. The effect of theaflavins on gingipain and collagenase activities of P. gingivalis was evaluated using synthetic chromogenic peptides and fluorogenic substrate. Human gingival fibroblasts (HGFs) were stimulated with P. gingivalis in the presence or absence of theaflavins, and then MMP-1, -2 secretion and their mRNA expression were assessed using an enzyme-linked immunosorbent assay (ELISA) and real-time PCR analysis, respectively. RESULTS: Theaflavins exhibited the antimicrobial effects against both planktonic culture and biofilm of P. gingivalis. Theaflavins also markedly inhibited the proteinase activities of P. gingivalis collagenase and gingipains in a dose-dependent manner. Lastly, theaflavins significantly inhibited the secretion and mRNA expression of MMP-1 and MMP-2 by HGFs stimulated with P. gingivalis. CONCLUSION: Theaflavins can affect the virulent properties of P. gingivalis and attenuate the MMP-mediated inflammatory response induced by this pathogen, which suggests that theaflavins may be potentially valuable supplementary therapeutic agent for prevention and treatment of P. gingivalis-associated periodontal diseases. SN - 1879-1506 UR - https://www.unboundmedicine.com/medline/citation/25244614/Theaflavins_inhibit_pathogenic_properties_of_P__gingivalis_and_MMPs_production_in_P__gingivalis_stimulated_human_gingival_fibroblasts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9969(14)00238-6 DB - PRIME DP - Unbound Medicine ER -