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[Identification of plantaginis semen based on ITS2 and psbA-trnH sequences].
Zhongguo Zhong Yao Za Zhi. 2014 Jun; 39(12):2227-32.ZZ

Abstract

In order to evaluate the efficiency of ITS2 and psbA-trnH sequences used as DNA barcodes to distinguish Plantaginis Semen from its adulterants, we collected 71 samples of Plantaginis Semen and its adulterants. The ITS2 and psbA-trnH sequences were aligned through Clustal W, and the genetic distances were calculated by kimura 2-parameter (K2P) model and the Neighbor-Joining (NJ) phylogenetic trees were constructed using MEGA 5.1. The results indicated that the ITS2 sequence lengths of Plantago asiatica and P. depressa were 199 bp and 200 bp, respectively; the maximum intra-specific K2P distance were lower than the minimum inter-specific K2P distance; the NJ tree based on ITS2 sequence indicated that Plantaginis Semen and its adulterants could be distinguished clearly. The sequence lengths of psbA-trnH of both P. asiatica and P. depressa were 340 bp; the maximum intra-specific K2P distances were lower than the minimum inter-specific K2P distance; the NJ tree based on psbA-trnH sequence showed that Plantaginis Semen can be distinguished clearly from its adulterants except for P. major. Therefore, ITS2 sequences can be used as an ideal DNA barcode to distinguish Plantaginis Semen from its adulterants.

Authors

Song M
No affiliation info available
Zhang YQ
No affiliation info available
Lin YH
No affiliation info available
Tu Y
No affiliation info available
Ma XX
No affiliation info available
Sun W
No affiliation info available
Xiang L
No affiliation info available
Jiao WJ
No affiliation info available
Liu X
No affiliation info available

MeSH

Base SequenceDNA Barcoding, TaxonomicDNA, PlantDNA, Ribosomal SpacerDrug ContaminationDrugs, Chinese HerbalMolecular Sequence DataPhylogenyPlant ProteinsPlantagoQuality ControlSeeds

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

25244750

Citation

Song, Ming, et al. "[Identification of Plantaginis Semen Based On ITS2 and psbA-trnH Sequences]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 39, no. 12, 2014, pp. 2227-32.
Song M, Zhang YQ, Lin YH, et al. [Identification of plantaginis semen based on ITS2 and psbA-trnH sequences]. Zhongguo Zhong Yao Za Zhi. 2014;39(12):2227-32.
Song, M., Zhang, Y. Q., Lin, Y. H., Tu, Y., Ma, X. X., Sun, W., Xiang, L., Jiao, W. J., & Liu, X. (2014). [Identification of plantaginis semen based on ITS2 and psbA-trnH sequences]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 39(12), 2227-32.
Song M, et al. [Identification of Plantaginis Semen Based On ITS2 and psbA-trnH Sequences]. Zhongguo Zhong Yao Za Zhi. 2014;39(12):2227-32. PubMed PMID: 25244750.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Identification of plantaginis semen based on ITS2 and psbA-trnH sequences]. AU - Song,Ming, AU - Zhang,Ya-Qin, AU - Lin,Yun-Han, AU - Tu,Yuan, AU - Ma,Xiao-Xi, AU - Sun,Wei, AU - Xiang,Li, AU - Jiao,Wen-Jing, AU - Liu,Xia, PY - 2014/9/24/entrez PY - 2014/9/24/pubmed PY - 2014/10/15/medline SP - 2227 EP - 32 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 39 IS - 12 N2 - In order to evaluate the efficiency of ITS2 and psbA-trnH sequences used as DNA barcodes to distinguish Plantaginis Semen from its adulterants, we collected 71 samples of Plantaginis Semen and its adulterants. The ITS2 and psbA-trnH sequences were aligned through Clustal W, and the genetic distances were calculated by kimura 2-parameter (K2P) model and the Neighbor-Joining (NJ) phylogenetic trees were constructed using MEGA 5.1. The results indicated that the ITS2 sequence lengths of Plantago asiatica and P. depressa were 199 bp and 200 bp, respectively; the maximum intra-specific K2P distance were lower than the minimum inter-specific K2P distance; the NJ tree based on ITS2 sequence indicated that Plantaginis Semen and its adulterants could be distinguished clearly. The sequence lengths of psbA-trnH of both P. asiatica and P. depressa were 340 bp; the maximum intra-specific K2P distances were lower than the minimum inter-specific K2P distance; the NJ tree based on psbA-trnH sequence showed that Plantaginis Semen can be distinguished clearly from its adulterants except for P. major. Therefore, ITS2 sequences can be used as an ideal DNA barcode to distinguish Plantaginis Semen from its adulterants. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/25244750/[Identification_of_plantaginis_semen_based_on_ITS2_and_psbA_trnH_sequences]_ DB - PRIME DP - Unbound Medicine ER -