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HPLC-DAD analysis, antioxidant potential and anti-urease activity of Asparagus gracilis collected from District Islamabad.
BMC Complement Altern Med. 2014 Sep 23; 14:347.BC

Abstract

BACKGROUND

Asparagus gracilis subspecie of Asparagus capitatus Baker, is described as food and medicine for various ailments. In this study we investigated, its phenolic constituents, in vitro antioxidant potential against various free radicals and anti-urease potential.

METHODS

Asparagus gracilis aerial parts collected from District Islamabad, Pakistan were extracted with crude methanol which was further fractionated into n-hexane, ethyl acetate, n-butanol and aqueous fraction. Total phenolic and flavonoid contents were estimated for extract and all the derived fractions. Diverse in vitro antioxidants assays such as DPPH, H2O2, •OH, ABTS, β-carotene bleaching assay, superoxide radical, lipid peroxidation, reducing power, and total antioxidant capacity were studied to assess scavenging potential. Antiurease activity of methanol extract and its derived fractions was also investigated. HPLC-DAD analysis of crude methanol extract was performed by using different phenolic standards.

RESULTS

Ethyl acetate fraction expressed maximum content of flavonoids (240.6 ± 6.1 mg RE/g dry sample), phenolics (615 ± 13 mg GAE/g dry sample) and best antioxidant potential among different fractions of crude methanol extract. Hydrogen peroxide assay and hydroxyl, supeoxide, nitric oxide free radicals antioxidant assays as well as beta carotene assay showed significant correlation with flavonoid content while hydrogen peroxide, ABTS and lipid peroxidation assay displayed significant correlation with phenolic content. HPLC analysis showed the presence of important phenolics i.e. catechin (4.04 ± 0.02 μg/mg sample), caffeic acid (0.89 ± 0.003 μg/mg sample), rutin (24.58 ± 0.1 μg/mg sample), myricetin (1.13 ± 0.07 μg/mg sample) and quercetin (14.91 ± 0.09 μg/mg sample). Ethyl acetate fraction expressed lowest IC50 in antiurease activity. Correlation analysis of antiurease activity expressed significant correlation with flavonoids (P < 0.004) and phenolics (P < 0.02) proposing multipotent activity of fractions.

CONCLUSION

These results revealed the presence of some bioactive compound in the ethyl acetate fraction having both antioxidant as well as antiurease potential.

Links

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Authors+Show Affiliations

Shah NA
No affiliation info available
Khan MR
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan. mrkanqau@yahoo.com.
Sattar S
No affiliation info available
Ahmad B
No affiliation info available
Mirza B
No affiliation info available

MeSH

AntioxidantsAsparagus PlantChromatography, High Pressure LiquidDNA DamageFlavonoidsPakistanPhenolsPlant ExtractsUrease

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25245231

Citation

Shah, Naseer Ali, et al. "HPLC-DAD Analysis, Antioxidant Potential and Anti-urease Activity of Asparagus Gracilis Collected From District Islamabad." BMC Complementary and Alternative Medicine, vol. 14, 2014, p. 347.
Shah NA, Khan MR, Sattar S, et al. HPLC-DAD analysis, antioxidant potential and anti-urease activity of Asparagus gracilis collected from District Islamabad. BMC Complement Altern Med. 2014;14:347.
Shah, N. A., Khan, M. R., Sattar, S., Ahmad, B., & Mirza, B. (2014). HPLC-DAD analysis, antioxidant potential and anti-urease activity of Asparagus gracilis collected from District Islamabad. BMC Complementary and Alternative Medicine, 14, 347. https://doi.org/10.1186/1472-6882-14-347
Shah NA, et al. HPLC-DAD Analysis, Antioxidant Potential and Anti-urease Activity of Asparagus Gracilis Collected From District Islamabad. BMC Complement Altern Med. 2014 Sep 23;14:347. PubMed PMID: 25245231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC-DAD analysis, antioxidant potential and anti-urease activity of Asparagus gracilis collected from District Islamabad. AU - Shah,Naseer Ali, AU - Khan,Muhammad Rashid, AU - Sattar,Saadia, AU - Ahmad,Bushra, AU - Mirza,Bushra, Y1 - 2014/09/23/ PY - 2014/04/21/received PY - 2014/09/19/accepted PY - 2014/9/24/entrez PY - 2014/9/24/pubmed PY - 2014/12/23/medline SP - 347 EP - 347 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 14 N2 - BACKGROUND: Asparagus gracilis subspecie of Asparagus capitatus Baker, is described as food and medicine for various ailments. In this study we investigated, its phenolic constituents, in vitro antioxidant potential against various free radicals and anti-urease potential. METHODS: Asparagus gracilis aerial parts collected from District Islamabad, Pakistan were extracted with crude methanol which was further fractionated into n-hexane, ethyl acetate, n-butanol and aqueous fraction. Total phenolic and flavonoid contents were estimated for extract and all the derived fractions. Diverse in vitro antioxidants assays such as DPPH, H2O2, •OH, ABTS, β-carotene bleaching assay, superoxide radical, lipid peroxidation, reducing power, and total antioxidant capacity were studied to assess scavenging potential. Antiurease activity of methanol extract and its derived fractions was also investigated. HPLC-DAD analysis of crude methanol extract was performed by using different phenolic standards. RESULTS: Ethyl acetate fraction expressed maximum content of flavonoids (240.6 ± 6.1 mg RE/g dry sample), phenolics (615 ± 13 mg GAE/g dry sample) and best antioxidant potential among different fractions of crude methanol extract. Hydrogen peroxide assay and hydroxyl, supeoxide, nitric oxide free radicals antioxidant assays as well as beta carotene assay showed significant correlation with flavonoid content while hydrogen peroxide, ABTS and lipid peroxidation assay displayed significant correlation with phenolic content. HPLC analysis showed the presence of important phenolics i.e. catechin (4.04 ± 0.02 μg/mg sample), caffeic acid (0.89 ± 0.003 μg/mg sample), rutin (24.58 ± 0.1 μg/mg sample), myricetin (1.13 ± 0.07 μg/mg sample) and quercetin (14.91 ± 0.09 μg/mg sample). Ethyl acetate fraction expressed lowest IC50 in antiurease activity. Correlation analysis of antiurease activity expressed significant correlation with flavonoids (P < 0.004) and phenolics (P < 0.02) proposing multipotent activity of fractions. CONCLUSION: These results revealed the presence of some bioactive compound in the ethyl acetate fraction having both antioxidant as well as antiurease potential. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/25245231/HPLC_DAD_analysis_antioxidant_potential_and_anti_urease_activity_of_Asparagus_gracilis_collected_from_District_Islamabad_ DB - PRIME DP - Unbound Medicine ER -