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Properties of AdeABC and AdeIJK efflux systems of Acinetobacter baumannii compared with those of the AcrAB-TolC system of Escherichia coli.
Antimicrob Agents Chemother. 2014 Dec; 58(12):7250-7.AA

Abstract

Acinetobacter baumannii contains RND-family efflux systems AdeABC and AdeIJK, which pump out a wide range of antimicrobial compounds, as judged from the MIC changes occurring upon deletion of the responsible genes. However, these studies may miss changes because of the high backgrounds generated by the remaining pumps and by β-lactamases, and it is unclear how the activities of these pumps compare quantitatively with those of the well-studied AcrAB-TolC system of Escherichia coli. We expressed adeABC and adeIJK of A. baumannii, as well as E. coli acrAB, in an E. coli host from which acrAB was deleted. The A. baumannii pumps were functional in E. coli, and the MIC changes that were observed largely confirmed the substrate range already reported, with important differences. Thus, the AdeABC system pumped out all β-lactams, an activity that was often missed in deletion studies. When the expression level of the pump genes was adjusted to a similar level for a comparison with AcrAB-TolC, we found that both A. baumannii efflux systems pumped out a wide range of compounds, but AdeABC was less effective than AcrAB-TolC in the extrusion of lipophilic β-lactams, novobiocin, and ethidium bromide, although it was more effective at tetracycline efflux. AdeIJK was remarkably more effective than a similar level of AcrAB-TolC in the efflux of β-lactams, novobiocin, and ethidium bromide, although it was less so in the efflux of erythromycin. These results thus allow us to compare these efflux systems on a quantitative basis, if we can assume that the heterologous systems are fully functional in the E. coli host.

Authors+Show Affiliations

Department of Molecular and Cell Biology, University of California, Berkeley, California, USA.Department of Molecular and Cell Biology, University of California, Berkeley, California, USA nhiroshi@berkeley.edu.

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

25246403

Citation

Sugawara, Etsuko, and Hiroshi Nikaido. "Properties of AdeABC and AdeIJK Efflux Systems of Acinetobacter Baumannii Compared With Those of the AcrAB-TolC System of Escherichia Coli." Antimicrobial Agents and Chemotherapy, vol. 58, no. 12, 2014, pp. 7250-7.
Sugawara E, Nikaido H. Properties of AdeABC and AdeIJK efflux systems of Acinetobacter baumannii compared with those of the AcrAB-TolC system of Escherichia coli. Antimicrob Agents Chemother. 2014;58(12):7250-7.
Sugawara, E., & Nikaido, H. (2014). Properties of AdeABC and AdeIJK efflux systems of Acinetobacter baumannii compared with those of the AcrAB-TolC system of Escherichia coli. Antimicrobial Agents and Chemotherapy, 58(12), 7250-7. https://doi.org/10.1128/AAC.03728-14
Sugawara E, Nikaido H. Properties of AdeABC and AdeIJK Efflux Systems of Acinetobacter Baumannii Compared With Those of the AcrAB-TolC System of Escherichia Coli. Antimicrob Agents Chemother. 2014;58(12):7250-7. PubMed PMID: 25246403.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Properties of AdeABC and AdeIJK efflux systems of Acinetobacter baumannii compared with those of the AcrAB-TolC system of Escherichia coli. AU - Sugawara,Etsuko, AU - Nikaido,Hiroshi, Y1 - 2014/09/22/ PY - 2014/9/24/entrez PY - 2014/9/24/pubmed PY - 2015/7/2/medline SP - 7250 EP - 7 JF - Antimicrobial agents and chemotherapy JO - Antimicrob. Agents Chemother. VL - 58 IS - 12 N2 - Acinetobacter baumannii contains RND-family efflux systems AdeABC and AdeIJK, which pump out a wide range of antimicrobial compounds, as judged from the MIC changes occurring upon deletion of the responsible genes. However, these studies may miss changes because of the high backgrounds generated by the remaining pumps and by β-lactamases, and it is unclear how the activities of these pumps compare quantitatively with those of the well-studied AcrAB-TolC system of Escherichia coli. We expressed adeABC and adeIJK of A. baumannii, as well as E. coli acrAB, in an E. coli host from which acrAB was deleted. The A. baumannii pumps were functional in E. coli, and the MIC changes that were observed largely confirmed the substrate range already reported, with important differences. Thus, the AdeABC system pumped out all β-lactams, an activity that was often missed in deletion studies. When the expression level of the pump genes was adjusted to a similar level for a comparison with AcrAB-TolC, we found that both A. baumannii efflux systems pumped out a wide range of compounds, but AdeABC was less effective than AcrAB-TolC in the extrusion of lipophilic β-lactams, novobiocin, and ethidium bromide, although it was more effective at tetracycline efflux. AdeIJK was remarkably more effective than a similar level of AcrAB-TolC in the efflux of β-lactams, novobiocin, and ethidium bromide, although it was less so in the efflux of erythromycin. These results thus allow us to compare these efflux systems on a quantitative basis, if we can assume that the heterologous systems are fully functional in the E. coli host. SN - 1098-6596 UR - https://www.unboundmedicine.com/medline/citation/25246403/Properties_of_AdeABC_and_AdeIJK_efflux_systems_of_Acinetobacter_baumannii_compared_with_those_of_the_AcrAB_TolC_system_of_Escherichia_coli_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=25246403 DB - PRIME DP - Unbound Medicine ER -