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A novel wheat-Dasypyrum breviaristatum substitution line with stripe rust resistance.
Cytogenet Genome Res. 2014; 143(4):280-7.CG

Abstract

The introduction of genetic variation from wild and cultivated Triticeae species has been a long-standing approach for wheat improvement. Dasypyrum breviaristatum species harbor novel and agronomically important genes for resistance against multi-fungal diseases. The development of new wheat-D. breviaristatum introgression lines offers chances for the identification of stripe rust resistance gene(s). A wheat line, D11-5, was selected from a cross between wheat line MY11 and wheat-D. breviaristatum partial amphiploid TDH-2. It was characterized by FISH and PCR-based molecular markers. Chromosome counting revealed that the D11-5 line shows a hexaploid set of 2n = 6x = 42 chromosomes. FISH analysis using the Dasypyrum repetitive sequence pDb12H as a probe demonstrated that D11-5 contained a pair of D. breviaristatum chromosomes, while FISH with wheat D-genomic repetitive sequences revealed that the chromosome 2D was absent in D11-5. The functional molecular markers confirmed that the introduced D. breviaristatum chromosomes belong to the homoeologous group 2, indicating that D11-5 was a 2V(b) (2D) disomic substitution line. Field resistance showed that the introduced D. breviaristatum chromosomes 2V(b) were responsible for the stripe rust resistance at the adult plant stage. FISH, C-banding, and PCR-based molecular marker analysis indicated that the chromosome 2V(b) of D. breviaristatum was completely different from the chromosome 2V of D. villosum. The identified wheat-D. breviaristatum chromosome substitution line D11-5 may be applied to produce agronomically desirable stripe rust resistance germplasm.

Authors+Show Affiliations

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25247402

Citation

Li, Guang-Rong, et al. "A Novel wheat-Dasypyrum Breviaristatum Substitution Line With Stripe Rust Resistance." Cytogenetic and Genome Research, vol. 143, no. 4, 2014, pp. 280-7.
Li GR, Zhao JM, Li DH, et al. A novel wheat-Dasypyrum breviaristatum substitution line with stripe rust resistance. Cytogenet Genome Res. 2014;143(4):280-7.
Li, G. R., Zhao, J. M., Li, D. H., Yang, E. N., Huang, Y. F., Liu, C., & Yang, Z. J. (2014). A novel wheat-Dasypyrum breviaristatum substitution line with stripe rust resistance. Cytogenetic and Genome Research, 143(4), 280-7. https://doi.org/10.1159/000366051
Li GR, et al. A Novel wheat-Dasypyrum Breviaristatum Substitution Line With Stripe Rust Resistance. Cytogenet Genome Res. 2014;143(4):280-7. PubMed PMID: 25247402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel wheat-Dasypyrum breviaristatum substitution line with stripe rust resistance. AU - Li,Guang-Rong, AU - Zhao,Jin-Mei, AU - Li,Dong-Hai, AU - Yang,En-Nian, AU - Huang,Yu-Feng, AU - Liu,Cheng, AU - Yang,Zu-Jun, Y1 - 2014/09/18/ PY - 2014/05/02/accepted PY - 2014/9/24/entrez PY - 2014/9/24/pubmed PY - 2014/12/20/medline SP - 280 EP - 7 JF - Cytogenetic and genome research JO - Cytogenet Genome Res VL - 143 IS - 4 N2 - The introduction of genetic variation from wild and cultivated Triticeae species has been a long-standing approach for wheat improvement. Dasypyrum breviaristatum species harbor novel and agronomically important genes for resistance against multi-fungal diseases. The development of new wheat-D. breviaristatum introgression lines offers chances for the identification of stripe rust resistance gene(s). A wheat line, D11-5, was selected from a cross between wheat line MY11 and wheat-D. breviaristatum partial amphiploid TDH-2. It was characterized by FISH and PCR-based molecular markers. Chromosome counting revealed that the D11-5 line shows a hexaploid set of 2n = 6x = 42 chromosomes. FISH analysis using the Dasypyrum repetitive sequence pDb12H as a probe demonstrated that D11-5 contained a pair of D. breviaristatum chromosomes, while FISH with wheat D-genomic repetitive sequences revealed that the chromosome 2D was absent in D11-5. The functional molecular markers confirmed that the introduced D. breviaristatum chromosomes belong to the homoeologous group 2, indicating that D11-5 was a 2V(b) (2D) disomic substitution line. Field resistance showed that the introduced D. breviaristatum chromosomes 2V(b) were responsible for the stripe rust resistance at the adult plant stage. FISH, C-banding, and PCR-based molecular marker analysis indicated that the chromosome 2V(b) of D. breviaristatum was completely different from the chromosome 2V of D. villosum. The identified wheat-D. breviaristatum chromosome substitution line D11-5 may be applied to produce agronomically desirable stripe rust resistance germplasm. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/25247402/A_novel_wheat_Dasypyrum_breviaristatum_substitution_line_with_stripe_rust_resistance_ L2 - https://www.karger.com?DOI=10.1159/000366051 DB - PRIME DP - Unbound Medicine ER -