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Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging.
J Pharm Biomed Anal. 2015 Jan; 102:93-9.JP

Abstract

Health benefits of wine, especially with red wine, have been linked to the presence of a wide range of phenolic antioxidants. Thus, the aim of this study was to develop a simple, high performance thin layer chromatographic (HPTLC) method combined with high resolution digital plate images to visually compare multiple wine samples simultaneously on a single chromatographic plate and to quantify levels of gallic acid, caffeic acid, resveratrol and rutin, as representatives of the four different classes of phenolics found in wines. We also wanted to investigate the contribution of the investigated phenolic compounds to the total polyphenolic content (TPC) and total antioxidant capacity (TAC) of the wine samples. The average concentrations of caffeic acid, gallic acid, resveratrol, and rutin in the red wines were 2.15, 30.17, 0.59 and 2.47 mg/L respectively with their concentration below limit of quantification in the white wine samples. The highest concentration of resveratrol and rutin is found in the Cabernet and Shiraz wine samples. The amounts of gallic acid are correlated with TPC (r=0.58). Italian wines have the highest correlation between TPC and TAC (r=0.99) although they do not contain detectable amounts of resveratrol, they contain significant amount of rutin. Therefore, antioxidant properties might be associated with the presence of flavanols in these wines.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Agatonovic-Kustrin S
School of Pharmacy and Applied Science, La Trobe University, Edwards Rd, Bendigo 3550, Australia. Electronic address: s.kustrin@latrobe.edu.au.
Hettiarachchi CG
School of Pharmacy and Applied Science, La Trobe University, Edwards Rd, Bendigo 3550, Australia.
Morton DW
School of Pharmacy and Applied Science, La Trobe University, Edwards Rd, Bendigo 3550, Australia.
Razic S
Department of Analytical Chemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia.

MeSH

AntioxidantsCaffeic AcidsChromatography, Thin LayerGallic AcidPhenolsResveratrolRutinStilbenesWine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25255450

Citation

Agatonovic-Kustrin, Snezana, et al. "Analysis of Phenolics in Wine By High Performance Thin-layer Chromatography With Gradient Elution and High Resolution Plate Imaging." Journal of Pharmaceutical and Biomedical Analysis, vol. 102, 2015, pp. 93-9.
Agatonovic-Kustrin S, Hettiarachchi CG, Morton DW, et al. Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging. J Pharm Biomed Anal. 2015;102:93-9.
Agatonovic-Kustrin, S., Hettiarachchi, C. G., Morton, D. W., & Razic, S. (2015). Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging. Journal of Pharmaceutical and Biomedical Analysis, 102, 93-9. https://doi.org/10.1016/j.jpba.2014.08.031
Agatonovic-Kustrin S, et al. Analysis of Phenolics in Wine By High Performance Thin-layer Chromatography With Gradient Elution and High Resolution Plate Imaging. J Pharm Biomed Anal. 2015;102:93-9. PubMed PMID: 25255450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging. AU - Agatonovic-Kustrin,Snezana, AU - Hettiarachchi,Chandima G, AU - Morton,David W, AU - Razic,Slavica, Y1 - 2014/09/06/ PY - 2014/06/10/received PY - 2014/08/20/revised PY - 2014/08/27/accepted PY - 2014/9/26/entrez PY - 2014/9/26/pubmed PY - 2015/9/15/medline KW - AMD chamber KW - Gradient elution KW - HPTLC KW - High resolution plate imaging KW - Wine phenolics SP - 93 EP - 9 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 102 N2 - Health benefits of wine, especially with red wine, have been linked to the presence of a wide range of phenolic antioxidants. Thus, the aim of this study was to develop a simple, high performance thin layer chromatographic (HPTLC) method combined with high resolution digital plate images to visually compare multiple wine samples simultaneously on a single chromatographic plate and to quantify levels of gallic acid, caffeic acid, resveratrol and rutin, as representatives of the four different classes of phenolics found in wines. We also wanted to investigate the contribution of the investigated phenolic compounds to the total polyphenolic content (TPC) and total antioxidant capacity (TAC) of the wine samples. The average concentrations of caffeic acid, gallic acid, resveratrol, and rutin in the red wines were 2.15, 30.17, 0.59 and 2.47 mg/L respectively with their concentration below limit of quantification in the white wine samples. The highest concentration of resveratrol and rutin is found in the Cabernet and Shiraz wine samples. The amounts of gallic acid are correlated with TPC (r=0.58). Italian wines have the highest correlation between TPC and TAC (r=0.99) although they do not contain detectable amounts of resveratrol, they contain significant amount of rutin. Therefore, antioxidant properties might be associated with the presence of flavanols in these wines. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/25255450/Analysis_of_phenolics_in_wine_by_high_performance_thin_layer_chromatography_with_gradient_elution_and_high_resolution_plate_imaging_ DB - PRIME DP - Unbound Medicine ER -