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Cryptomphalus aspersa mollusc eggs extract promotes migration and prevents cutaneous ageing in keratinocytes and dermal fibroblasts in vitro.
Int J Cosmet Sci. 2015 Feb; 37(1):41-55.IJ

Abstract

BACKGROUND

The search of substances that minimize cutaneous ageing has increased in the last few years. Previous studies have described the regenerative properties of the secretion of the mollusc Cryptomphalus aspersa (C. aspersa) when applied topically.

OBJECTIVE

We evaluate the in vitro effects of a new product derived from the eggs of C. aspersa, IFC-CAF, on cell proliferation, migration, distribution of cytoskeletal proteins, production of extracellular components as well as its ability to prevent cutaneous ageing because of intrinsic or extrinsic factors (exposure to UVB) by determination of ageing markers.

METHODS

We have used the human keratinocyte cell line (HaCaT cells), primary dermal fibroblasts (HDF) and senescent dermal fibroblasts (SHDF). The effects of the compound on cell proliferation and on the cell cycle were determined by the MTT colorimetric assay, estimation of total protein and/or trypan blue test and by flow cytometry, respectively. We also studied cell migration using the wound-healing migration assay, whereas ELISA assays, Western Blot and immunofluorescence microscopy were carried out to test the expression of proteins related to cytoskeleton, extracellular matrix and with ageing.

RESULTS

We have found that IFC-CAF does not promote proliferation but induces migration of HaCaT, HDF and SHDF in a time- and dose-dependent manner; a better organization of cytoskeletal proteins (F-actin and vimentin) and promotes the production of extracellular components (fibronectin, collagen 1 and MMPs) and the adhesion to cell-substrate vinculin protein. IFC-CAF also prevents cutaneous ageing. The treatment decreases the expression of the ageing-related markers b-Gal, p53 and p16INK4 in SDDF cells, and improves cell survival after UVB irradiation and nuclear repair in HaCaT cells.

CONCLUSION

IFC-CAF has regenerative properties and protects against ageing factors being, therefore, a potential therapeutic agent for treating or preventing skin ageing.

Authors+Show Affiliations

Department of Biology, Faculty of Sciences, Universidad Autónoma de Madrid, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25256953

Citation

Espada, J, et al. "Cryptomphalus Aspersa Mollusc Eggs Extract Promotes Migration and Prevents Cutaneous Ageing in Keratinocytes and Dermal Fibroblasts in Vitro." International Journal of Cosmetic Science, vol. 37, no. 1, 2015, pp. 41-55.
Espada J, Matabuena M, Salazar N, et al. Cryptomphalus aspersa mollusc eggs extract promotes migration and prevents cutaneous ageing in keratinocytes and dermal fibroblasts in vitro. Int J Cosmet Sci. 2015;37(1):41-55.
Espada, J., Matabuena, M., Salazar, N., Lucena, S., Kourani, O., Carrasco, E., Calvo, M., Rodríguez, C., Reyes, E., González, S., & Juarranz, A. (2015). Cryptomphalus aspersa mollusc eggs extract promotes migration and prevents cutaneous ageing in keratinocytes and dermal fibroblasts in vitro. International Journal of Cosmetic Science, 37(1), 41-55. https://doi.org/10.1111/ics.12167
Espada J, et al. Cryptomphalus Aspersa Mollusc Eggs Extract Promotes Migration and Prevents Cutaneous Ageing in Keratinocytes and Dermal Fibroblasts in Vitro. Int J Cosmet Sci. 2015;37(1):41-55. PubMed PMID: 25256953.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cryptomphalus aspersa mollusc eggs extract promotes migration and prevents cutaneous ageing in keratinocytes and dermal fibroblasts in vitro. AU - Espada,J, AU - Matabuena,M, AU - Salazar,N, AU - Lucena,S, AU - Kourani,O, AU - Carrasco,E, AU - Calvo,M, AU - Rodríguez,C, AU - Reyes,E, AU - González,S, AU - Juarranz,A, Y1 - 2014/10/27/ PY - 2014/07/30/received PY - 2014/09/11/accepted PY - 2014/9/27/entrez PY - 2014/9/27/pubmed PY - 2015/10/1/medline KW - Cryptomphalus aspersa KW - UV protection KW - fibroblasts KW - keratinocytes KW - senescence SP - 41 EP - 55 JF - International journal of cosmetic science JO - Int J Cosmet Sci VL - 37 IS - 1 N2 - BACKGROUND: The search of substances that minimize cutaneous ageing has increased in the last few years. Previous studies have described the regenerative properties of the secretion of the mollusc Cryptomphalus aspersa (C. aspersa) when applied topically. OBJECTIVE: We evaluate the in vitro effects of a new product derived from the eggs of C. aspersa, IFC-CAF, on cell proliferation, migration, distribution of cytoskeletal proteins, production of extracellular components as well as its ability to prevent cutaneous ageing because of intrinsic or extrinsic factors (exposure to UVB) by determination of ageing markers. METHODS: We have used the human keratinocyte cell line (HaCaT cells), primary dermal fibroblasts (HDF) and senescent dermal fibroblasts (SHDF). The effects of the compound on cell proliferation and on the cell cycle were determined by the MTT colorimetric assay, estimation of total protein and/or trypan blue test and by flow cytometry, respectively. We also studied cell migration using the wound-healing migration assay, whereas ELISA assays, Western Blot and immunofluorescence microscopy were carried out to test the expression of proteins related to cytoskeleton, extracellular matrix and with ageing. RESULTS: We have found that IFC-CAF does not promote proliferation but induces migration of HaCaT, HDF and SHDF in a time- and dose-dependent manner; a better organization of cytoskeletal proteins (F-actin and vimentin) and promotes the production of extracellular components (fibronectin, collagen 1 and MMPs) and the adhesion to cell-substrate vinculin protein. IFC-CAF also prevents cutaneous ageing. The treatment decreases the expression of the ageing-related markers b-Gal, p53 and p16INK4 in SDDF cells, and improves cell survival after UVB irradiation and nuclear repair in HaCaT cells. CONCLUSION: IFC-CAF has regenerative properties and protects against ageing factors being, therefore, a potential therapeutic agent for treating or preventing skin ageing. SN - 1468-2494 UR - https://www.unboundmedicine.com/medline/citation/25256953/Cryptomphalus_aspersa_mollusc_eggs_extract_promotes_migration_and_prevents_cutaneous_ageing_in_keratinocytes_and_dermal_fibroblasts_in_vitro_ L2 - https://doi.org/10.1111/ics.12167 DB - PRIME DP - Unbound Medicine ER -