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Clonal dissemination of multilocus sequence type 11 Klebsiella pneumoniae carbapenemase - producing K. pneumoniae in a Chinese teaching hospital.
APMIS. 2015 Feb; 123(2):123-7.A

Abstract

Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae has disseminated rapidly in China. We aimed to analyze the molecular epidemiology of four KPC-producing K. pneumoniae strains isolated from a suspected clonal outbreak during a 3-month period and to track the dissemination of KPC-producing K. pneumonia retrospectively. We created antimicrobial susceptibility profiles using an automated broth microdilution system and broth microdilution methods. We screened carbapenemase and KPC phenotypes using the modified Hodge test and meropenem-boronic acid (BA) disk test, respectively. We identified β-lactamase genes with PCR and sequencing. We investigated clonal relatedness for epidemiological comparison using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). All isolates expressed multidrug resistance and yielded positive results for the modified Hodge and meropenem-BA disk tests. The isolates all carried blaKPC -2 , and coproduced CTX-M-type extended-spectrum β-lactamase. PFGE and MLST showed that the isolates were clonally related. The PFGE patterns of these isolates had ≥90% similarity. We found a single clone, sequence type (ST) 11, and its typical dissemination mode resembled clonal spread. The dissemination of KPC-producing K. pneumoniae is clonally related and there is probable local transmission of a successful ST11 clone.

Authors+Show Affiliations

Department of Clinical Laboratory, the Ninth People's Hospital, Jiaotong University, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25257726

Citation

Sun, Kangde, et al. "Clonal Dissemination of Multilocus Sequence Type 11 Klebsiella Pneumoniae Carbapenemase - Producing K. Pneumoniae in a Chinese Teaching Hospital." APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, vol. 123, no. 2, 2015, pp. 123-7.
Sun K, Chen X, Li C, et al. Clonal dissemination of multilocus sequence type 11 Klebsiella pneumoniae carbapenemase - producing K. pneumoniae in a Chinese teaching hospital. APMIS. 2015;123(2):123-7.
Sun, K., Chen, X., Li, C., Yu, Z., Zhou, Q., & Yan, Y. (2015). Clonal dissemination of multilocus sequence type 11 Klebsiella pneumoniae carbapenemase - producing K. pneumoniae in a Chinese teaching hospital. APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, 123(2), 123-7. https://doi.org/10.1111/apm.12313
Sun K, et al. Clonal Dissemination of Multilocus Sequence Type 11 Klebsiella Pneumoniae Carbapenemase - Producing K. Pneumoniae in a Chinese Teaching Hospital. APMIS. 2015;123(2):123-7. PubMed PMID: 25257726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clonal dissemination of multilocus sequence type 11 Klebsiella pneumoniae carbapenemase - producing K. pneumoniae in a Chinese teaching hospital. AU - Sun,Kangde, AU - Chen,Xu, AU - Li,Chunsheng, AU - Yu,Zhongmin, AU - Zhou,Qi, AU - Yan,Yuzhong, Y1 - 2014/09/25/ PY - 2014/05/23/received PY - 2014/07/25/accepted PY - 2014/9/27/entrez PY - 2014/9/27/pubmed PY - 2015/4/23/medline KW - Klebsiella pneumoniae KW - Klebsiella pneumoniae carbapenemase KW - multilocus sequence typing KW - pulsed-field gel electrophoresis SP - 123 EP - 7 JF - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica JO - APMIS VL - 123 IS - 2 N2 - Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae has disseminated rapidly in China. We aimed to analyze the molecular epidemiology of four KPC-producing K. pneumoniae strains isolated from a suspected clonal outbreak during a 3-month period and to track the dissemination of KPC-producing K. pneumonia retrospectively. We created antimicrobial susceptibility profiles using an automated broth microdilution system and broth microdilution methods. We screened carbapenemase and KPC phenotypes using the modified Hodge test and meropenem-boronic acid (BA) disk test, respectively. We identified β-lactamase genes with PCR and sequencing. We investigated clonal relatedness for epidemiological comparison using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). All isolates expressed multidrug resistance and yielded positive results for the modified Hodge and meropenem-BA disk tests. The isolates all carried blaKPC -2 , and coproduced CTX-M-type extended-spectrum β-lactamase. PFGE and MLST showed that the isolates were clonally related. The PFGE patterns of these isolates had ≥90% similarity. We found a single clone, sequence type (ST) 11, and its typical dissemination mode resembled clonal spread. The dissemination of KPC-producing K. pneumoniae is clonally related and there is probable local transmission of a successful ST11 clone. SN - 1600-0463 UR - https://www.unboundmedicine.com/medline/citation/25257726/Clonal_dissemination_of_multilocus_sequence_type_11_Klebsiella_pneumoniae_carbapenemase___producing_K__pneumoniae_in_a_Chinese_teaching_hospital_ L2 - https://doi.org/10.1111/apm.12313 DB - PRIME DP - Unbound Medicine ER -