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Piperlonguminine is neuroprotective in experimental rat stroke.
Int Immunopharmacol. 2014 Dec; 23(2):447-51.II

Abstract

Inflammatory damage plays an important role in cerebral ischemic pathogenesis and may represent a target for treatment. Piperlonguminine (PE) has been proved to have anti-inflammatory actions. In this study, we investigated the effects of PE on cultured neuronal cell line, SH-SY5Y in vitro and experimental rat ischemic stroke in vivo. For oxygen-glucose deprivation (OGD) and tumor necrosis factor-α (TNF-α) stimulated SH-SY5Y cell line in vitro, SH-SY5Y cells were incubated with PE. In vivo, rats were subjected to middle cerebral artery occlusion (MACO) for 1h, followed by reperfusion for 23 h. The results of this study showed that treatment of SH-SY5Y cells with PE reduced the OGD-induced cytotoxicity and apoptosis and blocked TNF-α-induced activation of NF-κB and MAPK. Intraperitoneal injection of PE (2.4 mg/kg) produced a significant neuroprotective potential in rats with cerebral ischemia. PE attenuated neurological deficit scores, brain infarct volume and brain water content in rats, and inhibited activation of NF-κB and MAPK. These data show that PE protects the brain against ischemic cerebral injury via alleviating blood-brain barrier (BBB) breakdown, which may be mediated via inhibiting NF-κB and MAPK signaling pathways.

Authors+Show Affiliations

First Affiliated Hospital, Heilongjiang University of Chinese Medicine, PR China.Department of Physiology, Heilongjiang University of Chinese Medicine, PR China.The First Affiliated Hospital, Harbin Medical University, Harbin, PR China.Atlantic Institute of Oriental Medicine (ATOM), FL, USA.Department of Formulas of Chinese Medicine, Heilongjiang University of Chinese Medicine, PR China.First Affiliated Hospital, Heilongjiang University of Chinese Medicine, PR China.First Affiliated Hospital, Heilongjiang University of Chinese Medicine, PR China; Department of Acupuncture, Heilongjiang University of Chinese Medicine, PR China. Electronic address: sunzhong_ren@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25257731

Citation

Yang, Tiansong, et al. "Piperlonguminine Is Neuroprotective in Experimental Rat Stroke." International Immunopharmacology, vol. 23, no. 2, 2014, pp. 447-51.
Yang T, Sun S, Wang T, et al. Piperlonguminine is neuroprotective in experimental rat stroke. Int Immunopharmacol. 2014;23(2):447-51.
Yang, T., Sun, S., Wang, T., Tong, X., Bi, J., Wang, Y., & Sun, Z. (2014). Piperlonguminine is neuroprotective in experimental rat stroke. International Immunopharmacology, 23(2), 447-51. https://doi.org/10.1016/j.intimp.2014.09.016
Yang T, et al. Piperlonguminine Is Neuroprotective in Experimental Rat Stroke. Int Immunopharmacol. 2014;23(2):447-51. PubMed PMID: 25257731.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Piperlonguminine is neuroprotective in experimental rat stroke. AU - Yang,Tiansong, AU - Sun,Shixiao, AU - Wang,Tiegang, AU - Tong,Xin, AU - Bi,Junhui, AU - Wang,Yulin, AU - Sun,Zhongren, Y1 - 2014/09/22/ PY - 2014/08/01/received PY - 2014/09/09/revised PY - 2014/09/10/accepted PY - 2014/9/27/entrez PY - 2014/9/27/pubmed PY - 2015/8/4/medline KW - Experimental stroke KW - Piperlonguminine KW - Rats KW - Signaling pathways SP - 447 EP - 51 JF - International immunopharmacology JO - Int Immunopharmacol VL - 23 IS - 2 N2 - Inflammatory damage plays an important role in cerebral ischemic pathogenesis and may represent a target for treatment. Piperlonguminine (PE) has been proved to have anti-inflammatory actions. In this study, we investigated the effects of PE on cultured neuronal cell line, SH-SY5Y in vitro and experimental rat ischemic stroke in vivo. For oxygen-glucose deprivation (OGD) and tumor necrosis factor-α (TNF-α) stimulated SH-SY5Y cell line in vitro, SH-SY5Y cells were incubated with PE. In vivo, rats were subjected to middle cerebral artery occlusion (MACO) for 1h, followed by reperfusion for 23 h. The results of this study showed that treatment of SH-SY5Y cells with PE reduced the OGD-induced cytotoxicity and apoptosis and blocked TNF-α-induced activation of NF-κB and MAPK. Intraperitoneal injection of PE (2.4 mg/kg) produced a significant neuroprotective potential in rats with cerebral ischemia. PE attenuated neurological deficit scores, brain infarct volume and brain water content in rats, and inhibited activation of NF-κB and MAPK. These data show that PE protects the brain against ischemic cerebral injury via alleviating blood-brain barrier (BBB) breakdown, which may be mediated via inhibiting NF-κB and MAPK signaling pathways. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/25257731/Piperlonguminine_is_neuroprotective_in_experimental_rat_stroke_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(14)00368-3 DB - PRIME DP - Unbound Medicine ER -