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Development of intestinal ion-transporting mechanisms during smoltification and seawater acclimation in Atlantic salmon Salmo salar.
J Fish Biol 2014; 85(4):1227-52JF

Abstract

This study investigated the expression of ion transporters involved in intestinal fluid absorption and presents evidence for developmental changes in abundance and tissue distribution of these transporters during smoltification and seawater (SW) acclimation of Atlantic salmon Salmo salar. Emphasis was placed on Na(+) , K(+) -ATPase (NKA) and Na(+) , K(+) , Cl(-) co-transporter (NKCC) isoforms, at both transcriptional and protein levels, together with transcription of chloride channel genes. The nka α1c was the dominant isoform at the transcript level in both proximal and distal intestines; also, it was the most abundant isoform expressed in the basolateral membrane of enterocytes in the proximal intestine. This isoform was also abundantly expressed in the distal intestine in the lower part of the mucosal folds. The protein expression of intestinal Nkaα1c increased during smoltification. Immunostaining was localized to the basal membrane of the enterocytes in freshwater (FW) fish, and re-distributed to a lateral position after SW entry. Two other Nka isoforms, α1a and α1b, were expressed in the intestine but were not regulated to the same extent during smoltification and subsequent SW transfer. Their localization in the intestinal wall indicates a house-keeping function in excitatory tissues. The absorptive form of the NKCC-like isoform (sub-apically located NKCC2 and/or Na(+) , Cl(-) co-transporter) increased during smoltification and further after SW transfer. The cellular distribution changed from a diffuse expression in the sub-apical regions during smoltification to clustering of the transporters closer to the apical membrane after entry to SW. Furthermore, transcript abundance indicates that the mechanisms necessary for exit of chloride ions across the basolateral membrane and into the lateral intercellular space are present in the form of one or more of three different chloride channels: cystic fibrosis transmembrane conductance regulator I and II and chloride channel 3.

Authors+Show Affiliations

Fish Endocrinology Laboratory, Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, 40530 Gothenburg, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25263190

Citation

Sundh, H, et al. "Development of Intestinal Ion-transporting Mechanisms During Smoltification and Seawater Acclimation in Atlantic Salmon Salmo Salar." Journal of Fish Biology, vol. 85, no. 4, 2014, pp. 1227-52.
Sundh H, Nilsen TO, Lindström J, et al. Development of intestinal ion-transporting mechanisms during smoltification and seawater acclimation in Atlantic salmon Salmo salar. J Fish Biol. 2014;85(4):1227-52.
Sundh, H., Nilsen, T. O., Lindström, J., Hasselberg-Frank, L., Stefansson, S. O., McCormick, S. D., & Sundell, K. (2014). Development of intestinal ion-transporting mechanisms during smoltification and seawater acclimation in Atlantic salmon Salmo salar. Journal of Fish Biology, 85(4), pp. 1227-52. doi:10.1111/jfb.12531.
Sundh H, et al. Development of Intestinal Ion-transporting Mechanisms During Smoltification and Seawater Acclimation in Atlantic Salmon Salmo Salar. J Fish Biol. 2014;85(4):1227-52. PubMed PMID: 25263190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of intestinal ion-transporting mechanisms during smoltification and seawater acclimation in Atlantic salmon Salmo salar. AU - Sundh,H, AU - Nilsen,T O, AU - Lindström,J, AU - Hasselberg-Frank,L, AU - Stefansson,S O, AU - McCormick,S D, AU - Sundell,K, PY - 2014/05/06/received PY - 2014/08/11/accepted PY - 2014/9/30/entrez PY - 2014/9/30/pubmed PY - 2014/11/13/medline KW - Na+/K+-ATPase KW - Na+/K+/2Cl− co-transporter KW - cystic fibrosis transmembrane conductance regulator KW - fluid absorption KW - osmoregulation KW - salmonid SP - 1227 EP - 52 JF - Journal of fish biology JO - J. Fish Biol. VL - 85 IS - 4 N2 - This study investigated the expression of ion transporters involved in intestinal fluid absorption and presents evidence for developmental changes in abundance and tissue distribution of these transporters during smoltification and seawater (SW) acclimation of Atlantic salmon Salmo salar. Emphasis was placed on Na(+) , K(+) -ATPase (NKA) and Na(+) , K(+) , Cl(-) co-transporter (NKCC) isoforms, at both transcriptional and protein levels, together with transcription of chloride channel genes. The nka α1c was the dominant isoform at the transcript level in both proximal and distal intestines; also, it was the most abundant isoform expressed in the basolateral membrane of enterocytes in the proximal intestine. This isoform was also abundantly expressed in the distal intestine in the lower part of the mucosal folds. The protein expression of intestinal Nkaα1c increased during smoltification. Immunostaining was localized to the basal membrane of the enterocytes in freshwater (FW) fish, and re-distributed to a lateral position after SW entry. Two other Nka isoforms, α1a and α1b, were expressed in the intestine but were not regulated to the same extent during smoltification and subsequent SW transfer. Their localization in the intestinal wall indicates a house-keeping function in excitatory tissues. The absorptive form of the NKCC-like isoform (sub-apically located NKCC2 and/or Na(+) , Cl(-) co-transporter) increased during smoltification and further after SW transfer. The cellular distribution changed from a diffuse expression in the sub-apical regions during smoltification to clustering of the transporters closer to the apical membrane after entry to SW. Furthermore, transcript abundance indicates that the mechanisms necessary for exit of chloride ions across the basolateral membrane and into the lateral intercellular space are present in the form of one or more of three different chloride channels: cystic fibrosis transmembrane conductance regulator I and II and chloride channel 3. SN - 1095-8649 UR - https://www.unboundmedicine.com/medline/citation/25263190/Development_of_intestinal_ion_transporting_mechanisms_during_smoltification_and_seawater_acclimation_in_Atlantic_salmon_Salmo_salar_ L2 - https://doi.org/10.1111/jfb.12531 DB - PRIME DP - Unbound Medicine ER -