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Effects of the calcium channel blockers Phα1β and ω-conotoxin MVIIA on capsaicin and acetic acid-induced visceral nociception in mice.
Pharmacol Biochem Behav. 2014 Nov; 126:97-102.PB

Abstract

The effects of intrathecal administration of the toxins Phα1β and ω-conotoxin MVIIA were investigated in visceral nociception induced by an intraperitoneal injection of acetic acid and an intracolonic application of capsaicin. The pretreatments for 2h with the toxins reduced the number of writhes or nociceptive behaviors compared with the control mice. Phα1β administration resulted in an Imax of 84±6 and an ID50 of 12 (5-27), and ω-conotoxin MVIIA resulted in an Imax of 82±9 and an ID50 of 11 (4-35) in the contortions induced by the intraperitoneal injection of acetic acid. The administration of Phα1β resulted in an Imax of 64±4 and an ID50 of 18 (9-38), and ω-conotoxin MVIIA resulted in an Imax of 71±9 and an ID50 of 9 (1-83) in the contortions induced by intracolonic capsaicin administration. Phα1β (100/site) or ω-conotoxin MVIIA (30pmol/site) pretreatments caused a reduction in CSF glutamate release in mice intraperitoneally injected with acetic acid or treated with intracolonic capsaicin. The toxin pretreatments reduced the ROS levels induced by intraperitoneal acetic acid injection. Phα1β, but not ω-conotoxin MVIIA, reduced significantly the ROS levels induced by intracolonic capsaicin administration.

PERSPECTIVE

Phα1β is a ω-toxin with high therapeutic index and a broader action on calcium channels. It shows analgesic effect in several rodents' models of pain, including visceral pain, suggesting that this toxin has the potential to be used in clinical setting as a drug in the control of persistent pathological pain.

Authors+Show Affiliations

Programa de Pós Graduação Biomedicina e Medicina, Instituto de Ensino e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil.Programa de Pós-Graduação em Genética e Toxicologia Aplicada (PPGGTA), Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil.Programa de Pós Graduação em Farmacologia Bioquimica e Molecular, Faculdade Medicina UFMG, Belo Horizonte, MG, Brazil.Programa de Pós Graduação Biomedicina e Medicina, Instituto de Ensino e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil.Programa de Pós Graduação Biomedicina e Medicina, Instituto de Ensino e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil.Programa de Pós Graduação em Farmacologia Bioquimica e Molecular, Faculdade Medicina UFMG, Belo Horizonte, MG, Brazil.Programa de Pós Graduação Biomedicina e Medicina, Instituto de Ensino e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil.Programa de Pós Graduação Biomedicina e Medicina, Instituto de Ensino e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil.Fundação Ezequiel Dias (FUNED), Belo Horizonte, MG, Brazil.Programa de Pós Graduação em Farmacologia UFSM, Santa Maria, RS, Brazil.Programa de Pós Graduação Biomedicina e Medicina, Instituto de Ensino e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil; Programa de Pós Graduação em Farmacologia Bioquimica e Molecular, Faculdade Medicina UFMG, Belo Horizonte, MG, Brazil. Electronic address: marcusvgomez@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25268314

Citation

Diniz, Danuza Montijo, et al. "Effects of the Calcium Channel Blockers Phα1β and Ω-conotoxin MVIIA On Capsaicin and Acetic Acid-induced Visceral Nociception in Mice." Pharmacology, Biochemistry, and Behavior, vol. 126, 2014, pp. 97-102.
Diniz DM, de Souza AH, Pereira EM, et al. Effects of the calcium channel blockers Phα1β and ω-conotoxin MVIIA on capsaicin and acetic acid-induced visceral nociception in mice. Pharmacol Biochem Behav. 2014;126:97-102.
Diniz, D. M., de Souza, A. H., Pereira, E. M., da Silva, J. F., Rigo, F. K., Romano-Silva, M. A., Binda, N., Castro, C. J., Cordeiro, M. N., Ferreira, J., & Gomez, M. V. (2014). Effects of the calcium channel blockers Phα1β and ω-conotoxin MVIIA on capsaicin and acetic acid-induced visceral nociception in mice. Pharmacology, Biochemistry, and Behavior, 126, 97-102. https://doi.org/10.1016/j.pbb.2014.09.017
Diniz DM, et al. Effects of the Calcium Channel Blockers Phα1β and Ω-conotoxin MVIIA On Capsaicin and Acetic Acid-induced Visceral Nociception in Mice. Pharmacol Biochem Behav. 2014;126:97-102. PubMed PMID: 25268314.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of the calcium channel blockers Phα1β and ω-conotoxin MVIIA on capsaicin and acetic acid-induced visceral nociception in mice. AU - Diniz,Danuza Montijo, AU - de Souza,Alessandra Hubner, AU - Pereira,Elizete Maria Rita, AU - da Silva,Juliana Figueira, AU - Rigo,Flavia Karine, AU - Romano-Silva,Marco Aurélio, AU - Binda,Nancy, AU - Castro,Célio J,Jr AU - Cordeiro,Marta Nascimento, AU - Ferreira,Juliano, AU - Gomez,Marcus Vinicius, Y1 - 2014/09/28/ PY - 2014/07/01/received PY - 2014/09/15/revised PY - 2014/09/20/accepted PY - 2014/10/1/entrez PY - 2014/10/1/pubmed PY - 2015/11/10/medline KW - Calcium channel blockers KW - Glutamate KW - ROS KW - Toxins KW - Visceral nociception SP - 97 EP - 102 JF - Pharmacology, biochemistry, and behavior JO - Pharmacol Biochem Behav VL - 126 N2 - UNLABELLED: The effects of intrathecal administration of the toxins Phα1β and ω-conotoxin MVIIA were investigated in visceral nociception induced by an intraperitoneal injection of acetic acid and an intracolonic application of capsaicin. The pretreatments for 2h with the toxins reduced the number of writhes or nociceptive behaviors compared with the control mice. Phα1β administration resulted in an Imax of 84±6 and an ID50 of 12 (5-27), and ω-conotoxin MVIIA resulted in an Imax of 82±9 and an ID50 of 11 (4-35) in the contortions induced by the intraperitoneal injection of acetic acid. The administration of Phα1β resulted in an Imax of 64±4 and an ID50 of 18 (9-38), and ω-conotoxin MVIIA resulted in an Imax of 71±9 and an ID50 of 9 (1-83) in the contortions induced by intracolonic capsaicin administration. Phα1β (100/site) or ω-conotoxin MVIIA (30pmol/site) pretreatments caused a reduction in CSF glutamate release in mice intraperitoneally injected with acetic acid or treated with intracolonic capsaicin. The toxin pretreatments reduced the ROS levels induced by intraperitoneal acetic acid injection. Phα1β, but not ω-conotoxin MVIIA, reduced significantly the ROS levels induced by intracolonic capsaicin administration. PERSPECTIVE: Phα1β is a ω-toxin with high therapeutic index and a broader action on calcium channels. It shows analgesic effect in several rodents' models of pain, including visceral pain, suggesting that this toxin has the potential to be used in clinical setting as a drug in the control of persistent pathological pain. SN - 1873-5177 UR - https://www.unboundmedicine.com/medline/citation/25268314/Effects_of_the_calcium_channel_blockers_Phα1β_and_ω_conotoxin_MVIIA_on_capsaicin_and_acetic_acid_induced_visceral_nociception_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-3057(14)00269-X DB - PRIME DP - Unbound Medicine ER -